Double‐layer PLLA/PEO_Chitosan nanofibrous mats containing Hypericum perforatum L. as an effective approach for wound treatment

Abstract

Despite a large number of wound dressing materials in the market, an ideal dressing able to re‐establish both the features and functions of the native skin remains a challenge. Concerning this, the present study aimed to produce a new double‐layer nanofibrous dressing material. The upper layer was fabricated by using Poly(l‐lactic acid; PLLA) to act as a hydrophobic protective barrier, while the highly porous lower layer composed of a blend of Poly(ethylene) oxide (PEO) and Chitosan (CS) containing Hypericum perforatum L. (HP) was produced to be used in the wound site, reduce the risk of infection, and enhance the healing process. The produced double‐layer material exhibited the required chemical, physical, and mechanical properties. Besides, the results showed an inhibitory effect on Staphylococcus aureus and Pseudomonas aeruginosa growth without providing any cytotoxicity on normal human dermal fibroblast (NHDF) cells. Therefore, these findings emphasize the potential use of this material as an antimicrobial wound dressing to treat skin lesions.

Synthesis, Optimal Fabrication, and Physico‐Mechanical Property Evaluation of PCL‐b‐PLLA Diblock Copolymer‐Based Nanoscale Roughness Textured Electrospun Mats

“…[ 56 ] Also, the PLLA IR spectrum exhibit characteristic bands attributable to CH stretch at 2998 and 2950 cm −1 , CO stretch at 1765 cm −1 , CO stretching vibration at 1100 cm −1 . [ 57–59 ]…”

“…[56] Also, the PLLA IR spectrum exhibit characteristic bands attributable to C-H stretch at 2998 and 2950 cm −1 , C=O stretch at 1765 cm −1 , C-O stretching vibration at 1100 cm −1 . [57][58][59] The IR spectrum of the PCL-b-PLLA diblock copolymer shown in Figure 4 exhibits a prominent band at 1758 cm −1 corresponding to C=O stretch for the PLLA segment and a shoulder band at 1728 cm −1 arising from the PCL block of the copolymer. The spectrum shows a clear shift in the absorption band values as compared to pristine PLLA and PCL which is in accordance with the reported literature.…”

An In Vitro Study of Antibacterial Properties of Electrospun Hypericum perforatum Oil-Loaded Poly(lactic Acid) Nonwovens for Potential Biomedical Applications

“…The functional groups of HPO, PLA, and HPO/PLA electrospun nonwoven fabrics were determined by ATR-FTIR spectroscopy (Figure 2). The characteristic peaks of the HPO observed at 3308 −1 , 2853 −1 , 1743 cm −1 , and 844 cm −1 were assigned to aromatic C-H stretching, aliphatic C-H stretching, C=O stretching, and aromatic rings, respectively [34,41]. The signal at 1237 cm −1 typically overlaps, most notably C-O ester, which is found in oils [77].…”

“…The cellulose-based fibrous dressing material for the treatment of acute wounds was produced from carboxymethyl cellulose (CMC)/poly (ethylene oxide) (PEO)/Hypericum perforatum solutions by needle-free electrospinning method [40]. The production of PEO and chitosan (CS) based fibrous containing Hypericum perforatum for wound dressings has been investigated [41]. A new wound dressing was fabricated by electrospinning using Hypericum perforatum oil into a mixture of biodegradable PCL/GE blended polymers [42].…”

Marine Biopolymers as Bioactive Functional Ingredients of Electrospun Nanofibrous Scaffolds for Biomedical Applications

“…Various bioactive compounds have been encapsulated into chitosan-based electrospun nanofibers to develop multifunctional scaffolds for enhanced antimicrobial protection and accelerated healing [ 170 , 171 , 172 , 173 , 174 , 175 , 176 ]. Curcumin–chitosan/PVA core–shell nanofibers with a polymer-free core were developed via coaxial electrospinning for wound healing and tissue engineering applications.…”