1990
DOI: 10.1007/bf01962875
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Double labelling of cell surface antigens with colloidal gold markers

Abstract: Particles of colloidal gold of different diameters (15 nm and 40) have been used to distinctively label different surface antigens expressed on the surface of human peripheral blood B and T lymphocytes. Silver enhancement has been used to facilitate the observation of the gold particles. Observations were carried out in the backscattered electron imaging mode of the scanning electron microscope. Two different methods have been compared: in Method I the two antigens have been identified by monoclonal antibodies… Show more

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Cited by 14 publications
(2 citation statements)
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“…Admittedly, imaging of surface morphology alone by routine SEM is far from an infallible way to distinguish between T-and B-derived lymphocytes. Expectedly, double immuno-labelling techniques (de Harven et al, 1990) will reveal more convincingly the B-or T-cell derivation of the rare B-ly7 + lymphocytes. Labelling with gold markers of distinct sizes (15 and 30 nm, for example, or 5 and 20 nm if a very high resolution SEM is available) should be performed for B-ly71CD3 and for B-ly7ICD20.…”
Section: Lymphocytesmentioning
confidence: 99%
“…Admittedly, imaging of surface morphology alone by routine SEM is far from an infallible way to distinguish between T-and B-derived lymphocytes. Expectedly, double immuno-labelling techniques (de Harven et al, 1990) will reveal more convincingly the B-or T-cell derivation of the rare B-ly7 + lymphocytes. Labelling with gold markers of distinct sizes (15 and 30 nm, for example, or 5 and 20 nm if a very high resolution SEM is available) should be performed for B-ly71CD3 and for B-ly7ICD20.…”
Section: Lymphocytesmentioning
confidence: 99%
“…BE1 is advantageous over the secondary electron imaging (SEI) in that the metal coating, needed for SEI, does not obscure the label either as a coating or as a competing element with high atomic number. The high backscatter electron (BE) yield of the gold particles thus offers high contrast, and smaller probes and a wider range of probe sizes can be applied, being ideal for double labeling (de Harven and Soligo, 1989;de Harven et al, 1990;Namork and Heier, 1989;Namork et al, 1987Namork et al, ,1988Namork, 1991;Pawley and Albrecht, 1988;Soligo et al, 1986). Indirect immunolabeling using primary antibodies of different immunoglobulin classes, species, or different ligands was always used in these studies to avoid cross reactivity.…”
Section: Introductionmentioning
confidence: 99%