1987
DOI: 10.1002/jemt.1060060111
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Double labeling of cell surface antigens imaged with backscattered electrons

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Cited by 8 publications
(5 citation statements)
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“…Hence, to diminish steric hindrance and to allow safe discrimination between two probe sizes in double-labeling experiments, 20 and 40 nm probes are the only feasible sizes to use. The simultaneous detection of two antigenic sites on cells labeled with 20 and 40 nm gold probes visualized in the BE1 mode has been reported (Namork et al, 1987;Soligo et al, 1986?. Silver enhancement has, since it was introduced by Danscher (1981?, frequently been applied in light microscopy (LM) and transmission electron microscopy (TEM? to intensify gold labels Birrell and Hedberg, 1987;Danscher et al, 1987;Holgate et al, 1983;Lackie et al, 1985;Scopsi and Larsson, 1985).…”
Section: Introductionmentioning
confidence: 91%
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“…Hence, to diminish steric hindrance and to allow safe discrimination between two probe sizes in double-labeling experiments, 20 and 40 nm probes are the only feasible sizes to use. The simultaneous detection of two antigenic sites on cells labeled with 20 and 40 nm gold probes visualized in the BE1 mode has been reported (Namork et al, 1987;Soligo et al, 1986?. Silver enhancement has, since it was introduced by Danscher (1981?, frequently been applied in light microscopy (LM) and transmission electron microscopy (TEM? to intensify gold labels Birrell and Hedberg, 1987;Danscher et al, 1987;Holgate et al, 1983;Lackie et al, 1985;Scopsi and Larsson, 1985).…”
Section: Introductionmentioning
confidence: 91%
“…The backscatter electron imaging (BEI) mode of the scanning electron microscope has proved very useful in the detection of colloidal gold probes labeling cell surface antigens. The method, first introduced by Trejdosiewicz et al in 1981, has found widespread application, sucessfully applied by many workers (Birrell and Hedberg, 1987;deHarven et al, 1984deHarven et al, , 1986deHarven et al, , 1987aGoode and Maugel, 1987;Heier et al, 1988;Hodges et al, 1987;Namork et al, 1986Namork et al, , 1987Nava et al, 1984;Soligo et al, 1985Soligo et al, , 1986Studer and Hermann, 1986;Walther et al, 1984;Walther and Muller, 1986). Few of the probe sizes commercially available (5-40 nm) are practical to use in scanning electron microscopy (SEMI, however, and the choice becomes even more limited when two probes are to be discriminated as in double-labeling of cells.…”
Section: Introductionmentioning
confidence: 99%
“…For double labeling of molecules exposed on cell surfaces we have com pared the value of two different methods, one using two identifier antibodies of different classes (one IgG and one IgM) as recently demonstrated by Namork et al (1987), and another using two ligands of unrelated chemistry (anti-immunoglobulin and biotin-streptavidin complex), as initially rec ommended by Yamada and Sano (1985). In both cases, the gold probes used were of different diameters (15 and 40 nm).…”
Section: Doubling Labelingmentioning
confidence: 99%
“…BE1 is advantageous over the secondary electron imaging (SEI) in that the metal coating, needed for SEI, does not obscure the label either as a coating or as a competing element with high atomic number. The high backscatter electron (BE) yield of the gold particles thus offers high contrast, and smaller probes and a wider range of probe sizes can be applied, being ideal for double labeling (de Harven and Soligo, 1989;de Harven et al, 1990;Namork and Heier, 1989;Namork et al, 1987Namork et al, ,1988Namork, 1991;Pawley and Albrecht, 1988;Soligo et al, 1986). Indirect immunolabeling using primary antibodies of different immunoglobulin classes, species, or different ligands was always used in these studies to avoid cross reactivity.…”
Section: Introductionmentioning
confidence: 99%