Double crosslinked biomimetic composite hydrogels containing topographical cues and WAY-316606 induce neural tissue regeneration and functional recovery after spinal cord injury

Zhao, Xingchang; Lu, Xianzhe; Li, Kai; Song, Shiqiang; Zhang, Luo; Zheng, Chuanchuan; Yang, Chengliang; Wang, Xiumei; Wang, Liqiang; Tang, Yue; Wang, Chong; Liu, Jia

doi:10.1016/j.bioactmat.2022.12.024

Cited by 8 publications

(7 citation statements)

References 44 publications

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“…Scaffolds used to stimulate axonal connections in the wounded spinal cord should aim to match native tissue anisotropy and supply varied guidance cues along the head-caudal axis, but traditional fabrication approaches struggle to provide these features. 3D bioprinting technology can employ multiple print heads to accurately simulate changes in the structural and mechanical characteristics of gray and white matter by varying the material type, material concentration, and crosslinking intensity along the length of the scaffold [49,61,62] (figures 2(C) and (D)). These heterogeneous scaffolds can more accurately imitate the anisotropic properties of the spinal cord, thereby enhancing neural network regeneration and functional recovery following SCI [63].…”

Section: Mechanical Customizationmentioning

confidence: 99%

“…Nevertheless, gelatin also has poor mechanical properties when used alone [137], often necessitating combination with other biomaterials such as chitosan and fibroin during bioink development [54,85]. Other types of natural materials have been used to produce bioprinted scaffolds for spinal cord repair, such as sodium alginate, hyaluronic acid, and acellular matrix [61,96,102,116,121]. These natural materials may provide different tissue repair effects and mechanisms when used individually or in combination, but all exhibit a minimal immunogenic response and may help to reduce the inflammatory reaction caused by scaffold transplantation [139].…”

Section: −Hydrophobicity −Slow Degradation Rate −Inhibits Cell Adhesionmentioning

confidence: 99%

“…For example, PCL can be easily formed into microfibers using 3D printing and help with axon guidance during spinal cord repair. These directional microfibers allow neurons within the lesion to disseminate along the fiber direction, and their axons to break through the scar barrier to join the two ends of the lesion, resulting in functional repair [61]. Due to its hydrophobicity, PCL alone may result in poor cell adhesion and survival, and for this reason it is often combined with other materials when formulating scaffolds for spinal cord repair [105,145].…”

Section: Synthetic Materialsmentioning

confidence: 99%

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3D bioprinting approaches for spinal cord injury repair

Jiu,

Liu,

et al. 2024

Biofabrication

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Regenerative healing of spinal cord injury poses an ongoing medical challenge by causing persistent neurological impairment and a significant socioeconomic burden. The complexity of spinal cord tissue presents hurdles to successful regeneration following injury, due to the difficulty of forming a biomimetic structure that faithfully replicates native tissue using conventional tissue engineering scaffolds. 3D bioprinting is a rapidly evolving technology with unmatched potential to create 3D biological tissues with complicated and hierarchical structure and composition. With the addition of biological additives such as cells and biomolecules, 3D bioprinting can fabricate preclinical implants, tissue or organ-like constructs, and in vitro models through precise control over the deposition of biomaterials and other building blocks. This review highlights the characteristics and advantages of 3D bioprinting for scaffold fabrication to enable spinal cord injury repair, including bottom-up manufacturing, mechanical customization, and spatial heterogeneity. This review also critically discusses the impact of various fabrication parameters on the efficacy of spinal cord repair using 3D bioprinted scaffolds, including the choice of printing method, scaffold shape, biomaterials, and biological supplements such as cells and growth factors. High-quality preclinical studies are required to accelerate the translation of 3D bioprinting into clinical practice for spinal cord repair. Meanwhile, other technological advances will continue to improve the regenerative capability of bioprinted scaffolds, such as the incorporation of nanoscale biological particles and the development of 4D printing.

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Section: Mechanical Customizationmentioning

confidence: 99%

Section: −Hydrophobicity −Slow Degradation Rate −Inhibits Cell Adhesionmentioning

confidence: 99%

Section: Synthetic Materialsmentioning

confidence: 99%

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3D bioprinting approaches for spinal cord injury repair

Jiu,

Liu,

et al. 2024

Biofabrication

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“…It is characterized by a hydrophilic exterior and an internal cavity that can be loaded with pharmacologic agents [29]. It has been used as a component of small molecule-loaded hydrogels for the repair of spinal cord injury [33,34].…”

Section: Nature-derived Polymersmentioning

confidence: 99%

Current Biomedical Applications of 3D-Printed Hydrogels

Barcena,

Dhal,

Patel

et al. 2023

Gels

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Three-dimensional (3D) printing, also known as additive manufacturing, has revolutionized the production of physical 3D objects by transforming computer-aided design models into layered structures, eliminating the need for traditional molding or machining techniques. In recent years, hydrogels have emerged as an ideal 3D printing feedstock material for the fabrication of hydrated constructs that replicate the extracellular matrix found in endogenous tissues. Hydrogels have seen significant advancements since their first use as contact lenses in the biomedical field. These advancements have led to the development of complex 3D-printed structures that include a wide variety of organic and inorganic materials, cells, and bioactive substances. The most commonly used 3D printing techniques to fabricate hydrogel scaffolds are material extrusion, material jetting, and vat photopolymerization, but novel methods that can enhance the resolution and structural complexity of printed constructs have also emerged. The biomedical applications of hydrogels can be broadly classified into four categories—tissue engineering and regenerative medicine, 3D cell culture and disease modeling, drug screening and toxicity testing, and novel devices and drug delivery systems. Despite the recent advancements in their biomedical applications, a number of challenges still need to be addressed to maximize the use of hydrogels for 3D printing. These challenges include improving resolution and structural complexity, optimizing cell viability and function, improving cost efficiency and accessibility, and addressing ethical and regulatory concerns for clinical translation.

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“…Spinal cord injury (SCI) is a condition affecting the central nervous system and carries a significant risk of disability and mortality. Furthermore, the incidence of SCI is on the rise [ 1 – 3 ]. Trauma is the most common cause of SCI in clinical cases [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

The application of stem cell sheets for neuronal regeneration after spinal cord injury: a systematic review of pre-clinical studies

Xu,

Zhao,

Yang

et al. 2023

Syst Rev

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Background Stem cell sheet implantation offers a promising avenue for spinal cord injury (SCI) and is currently under investigation in pre-clinical in vivo studies. Nevertheless, a systematic review of the relevant literature is yet to be performed. Thus, this systematic review aims to explore the efficacy of stem cell sheet technology in treating SCI, as indicated by experimental animal model studies. Methods We searched PubMed, EMBASE, and Web of Science. Manuscripts that did not pertain to in vivo pre-clinical studies and those published in non-English languages were excluded. A risk assessment for bias was performed using the SYRCLE tool. Extracted data were synthesized only qualitatively because the data were not suitable for conducting the meta-analysis. Results Among the 847 studies retrieved from electronic database searches, seven met the inclusion criteria. Six of these studies employed a complete transection model, while one utilized a compression model. Stem cell sources included bone marrow mesenchymal stem cells, stem cells from human exfoliated deciduous teeth, and adipose-derived mesenchymal stem cells. In all included studies, stem cell sheet application significantly improved motor and sensory functional scores compared to intreated SCI rats. This functional recovery correlated with histological improvements at the injury site. All studies are at low risk of bias but certain domains were not reported by some or all of the studies. Conclusion The results of our systematic review suggest that stem cell sheets may be a feasible therapeutic approach for the treatment of SCI. Future research should be conducted on stem cell sheets in various animal models and types of SCI, and careful validation is necessary before translating stem cell sheets into clinical studies.

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Double crosslinked biomimetic composite hydrogels containing topographical cues and WAY-316606 induce neural tissue regeneration and functional recovery after spinal cord injury

Cited by 8 publications

References 44 publications

3D bioprinting approaches for spinal cord injury repair

3D bioprinting approaches for spinal cord injury repair

Current Biomedical Applications of 3D-Printed Hydrogels

The application of stem cell sheets for neuronal regeneration after spinal cord injury: a systematic review of pre-clinical studies

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