Dothideomycetes and Leotiomycetes sterile mycelia isolated from the Italian seagrass Posidonia oceanica based on rDNA data

Varese, Giovanna Cristina; Ercole, Enrico; Panno, Luigi; Vizzini, Alfredo

doi:10.1186/2193-1801-3-508

Cited by 41 publications

(42 citation statements)

References 68 publications

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“…Interest in mycoviruses stems from their role as edible mushroom pathogens (Hollings, 1962), as cause of fermentation 'diseases' in biotechnological exploitation of fungi (Buck et al, 1971), as cause of hypovirulence in important fungal pathogens (Xie and Jiang, 2014) and more recently, for their possible ecological role in adaptation to extreme environments (Marquez et al, 2007). In previous work, we identified the virome associated to a collection of fungal isolates from different districts of the marine plant Posidonia oceanica (Gnavi et al, 2014): in six distinct fungal strains, we identified 12 new putative virus species of ssRNA and dsRNA genomes (Nerva et al, 2016). Remarkably, a single-conidia isolate of Penicillium aurantiogriseum var.…”

Section: Introductionmentioning

confidence: 99%

Transmission of Penicillium aurantiogriseum partiti‐like virus 1 to a new fungal host (Cryphonectria parasitica) confers higher resistance to salinity and reveals adaptive genomic changes

Nerva

Silvestri

Ciuffo

et al. 2017

Environmental Microbiology

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We attempted to transfect six recently characterized virus species to protoplasts of Penicillium janczewskii and Chryphonectria parasitica. None of the recovered P. janczewskii colonies was positive for the transfected viruses, but Penicillium aurantiogriseum partiti-like virus 1 (PaPLV1) was detected in three distinct regenerated C. parasitica colonies. We screened the phenotype of the infected strains in up to 45 different conditions combining different media, salinity and temperatures: our results show that the infected strains grow slower than the virus- free in most of the tested conditions with the exception of halophilic stress in a specific nutrient combination media. We proceeded to characterize molecularly the population of distinct isolates of PaPLV1 infected C. parasitica through RNAseq: comparison to the viral population present in the original host - P. auratiogriseum - showed that two isolates accumulated non-synonymous mutations suggesting adaptation to the new host. RNAseq analyses identified a second genomic RNA segment and northern blot of RNA extracted from purified virus suspensions allowed establishing that PaPLV1 is at least bipartite in nature and that it forms isometric virions of circa 36-38 nm in diameter. In light of these new acquisitions, we discuss the taxonomic placement of PaPLV1 inside the Partitiviridae.

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Section: Introductionmentioning

confidence: 99%

Transmission of Penicillium aurantiogriseum partiti‐like virus 1 to a new fungal host (Cryphonectria parasitica) confers higher resistance to salinity and reveals adaptive genomic changes

Nerva

Silvestri

Ciuffo

et al. 2017

Environmental Microbiology

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“…In this work we are reporting 12 new viral species resulting from the analysis of 91 isolates representing various fungal taxonomic groups and species (Panno et al, 2013 andGnavi et al, 2014) from a well characterized collection previously described in its mycological aspects and associated with the seagrass P. oceanica ( Panno et al, 2013).…”

Section: A Collection Of New Mycovirus Species Isolated From Marine Fmentioning

confidence: 99%

“…We decided, therefore, to screen for the presence of virus and plasmid symbionts in a collection of marine fungi isolated from the seagrass Posidonia oceanica ( Panno et al, 2013 andGnavi et al, 2014). P. oceanica is a protected species of utmost importance for sea ecology as exemplified by specific European legislation addressing this issue ( EEC 92/43, 1992).…”

Section: Introductionmentioning

confidence: 99%

Multiple approaches for the detection and characterization of viral and plasmid symbionts from a collection of marine fungi

Nerva¹,

et al. 2016

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Highlights• First report of mycoviruses isolated from fungi from marine environment.• Survey of mycoviruses using multiple approaches for both DNA and RNA genomes.• RNAseq analysis is superior to sRNA for de novo assembly of mycoviruses.• Twelve new virus species were characterized molecularly.• Expression of a viral RNA from an endogenized cDNA segment. AbstractThe number of reported mycoviruses is increasing exponentially due to the current ability to detect mycoviruses using next-generation sequencing (NGS) approaches, with a large number of viral genomes built in-silico using data from fungal transcriptome projects. We decided to screen a collection of fungi originating from a specific marine environment (associated with the seagrass Posidonia oceanica) for the presence of mycoviruses: our findings reveal a wealth of diversity among these symbionts and this complexity will require further studies to address their specific role in this ecological niche. In specific, we identified twelve new virus species belonging to nine distinct lineages: they are members of megabirnavirus, totivirus, chrysovirus, partitivirus and five still undefined clades. We showed evidence of an endogenized virus ORF, and evidence of accumulation of dsRNA from metaviridae retroviral elements. We applied different techniques for detecting the presence of mycoviruses including (i) dsRNA extraction and cDNA cloning, (ii) small and total RNA sequencing through NGS techniques, (iii) rolling circle amplification (RCA) and total DNA extraction analyses, (iv) virus purifications and electron microscopy. We tried also to critically evaluate the intrinsic value and limitations of each of these techniques. Based on the samples we could compare directly, RNAseq analysis is superior to sRNA for de novo assembly of mycoviruses. To our knowledge this is the first report on the virome of fungi isolated from marine environment.

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“…Marine fungi were isolated and identified from the seagrass Posidonia oceanica, the green alga Flabellia petiolata and the brown alga Padina pavonica collected nearby Elba Island in the Mediterranean Sea as previously described [20], [21] and [22] and are preserved at the Mycoteca Universitatis Taurinensis (MUT).…”

Section: Marine Fungimentioning

confidence: 99%

Marine fungi as source of new hydrophobins

Cicatiello

Gravagnuolo

Varese

et al. 2016

International Journal of Biological Macromolecules

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Highlights23 Marine fungi have been analyzed to identify hydrophobin producers.6 New hydrophobins have been isolated. 4 Proteins belong to class I and 2 to class II hydrophobins.One of the new class I hydrophobins stably changes the wettability of a crystalline silicon chip. One of the new class II hydrophobin is endowed with remarkable emulsification capacity. AbstractHydrophobins have been described as the most powerful surface-active proteins known. They are produced by filamentous fungi and exhibit a distinct amphiphilic structure determining their selfassembly at hydrophilic-hydrophobic interfaces and surfactant properties which have been demonstrated to be useful for several biotechnological applications. The marine environment represents a vast natural resource of new molecules produced by organisms growing in various stressful conditions. This study was focused on the screening of 100 marine fungi from Mycoteca Universitatis Taurinensis (MUT) for the identification of new hydrophobins. Four different methods were set up to extract hydrophobins of class I and II, from the mycelium or the culture broth of fungi. Six fungi were selected as the best producers of hydrophobins endowed with different characteristics. Their ability to form stable amphiphilic films and their emulsification capacity in the presence of olive oil was evaluated. Figure options Graphical abstract

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Dothideomycetes and Leotiomycetes sterile mycelia isolated from the Italian seagrass Posidonia oceanica based on rDNA data

Cited by 41 publications

References 68 publications

Transmission of Penicillium aurantiogriseum partiti‐like virus 1 to a new fungal host (Cryphonectria parasitica) confers higher resistance to salinity and reveals adaptive genomic changes

Transmission of Penicillium aurantiogriseum partiti‐like virus 1 to a new fungal host (Cryphonectria parasitica) confers higher resistance to salinity and reveals adaptive genomic changes

Multiple approaches for the detection and characterization of viral and plasmid symbionts from a collection of marine fungi

Marine fungi as source of new hydrophobins

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