Dot Scanner: open‐source software for quantitative live‐cell imaging in planta
Holly Allen,
Brian Davis,
Jenna Patel
et al.
Abstract:SUMMARYConfocal microscopy has greatly aided our understanding of the major cellular processes and trafficking pathways responsible for plant growth and development. However, a drawback of these studies is that they often rely on the manual analysis of a vast number of images, which is time‐consuming, error‐prone, and subject to bias. To overcome these limitations, we developed Dot Scanner, a Python program for analyzing the densities, lifetimes, and displacements of fluorescently tagged particles in an unbias… Show more
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