Dot immunogold filtration assay (DIGFA) with multiple native antigens for rapid serodiagnosis of human cystic and alveolar echinococcosis

Xia, Feng; Wen, Hao; Zhang, Zhaoxia; Chen, Xinhua; Ma, Xudong; Zhang, Jinping; Qi, Xinwei; Bradshaw, Helen; Vuitton, Dominique A.; Craig, Philip S.

doi:10.1016/j.actatropica.2009.10.003

Cited by 57 publications

(59 citation statements)

References 27 publications

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“…The isolated analysis of the AgB-dot had a sensitivity and specificity for CE of 68.4 and 93.4%, respectively, whereas the isolated analysis of the Em2-dot diagnosed 83.3% of AE patients with a specificity of 90.3% and reliability for species differentiation of 83.0% [50]. Although the overall performances of these tests were lower than those in our study, differences in the epidemiological background and serum panel used have to be taken into account.…”

Section: Discussionmentioning

confidence: 99%

“…The parallel use of multiple native antigens (EgHF, EgP, AgB and Em2) in a dot-immunogold filtration assay (DIGFA) analysing patients with probable or confirmed CE or AE yielded sensitivities for CE and AE of 80.7 and 92.9%, respectively, with a specificity of 89.6% [50]. The isolated analysis of the AgB-dot had a sensitivity and specificity for CE of 68.4 and 93.4%, respectively, whereas the isolated analysis of the Em2-dot diagnosed 83.3% of AE patients with a specificity of 90.3% and reliability for species differentiation of 83.0% [50].…”

Section: Discussionmentioning

confidence: 99%

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Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

et al. 2011

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PURPOSE: Human alveolar (AE) and cystic echinococcosis (CE) caused by the metacestode stages of Echinococcus multilocularis and E. granulosus, respectively, lack pathognomonic clinical signs. Diagnosis therefore relies on the results of imaging and serological studies. The primary goal of this study was to evaluate the efficacy of several easy-to-produce crude or partially purified E. granulosus and E. multilocularis metacestode-derived antigens as tools for the serological diagnosis and differential diagnosis of patients suspicious for AE or CE. METHODS: The sera of 51 treatment-naïve AE and 32 CE patients, 98 Swiss blood donors and 38 patients who were initially suspicious for echinococcosis but suffering from various other liver diseases (e.g., liver neoplasia, etc.) were analysed. RESULTS: According to the results of enzyme-linked immunosorbent assays (ELISA), metacestode-derived antigens of E. granulosus had sensitivities varying from 81 to 97% and >99.9% for the diagnosis of CE and AE, respectively. Antigens derived from E. multilocularis metacestodes had sensitivities ranging from 84 to 91% and >99.9% for the diagnosis of CE and AE, respectively. Specificities ranged from 92 to >99.9%. Post-test probabilities for the differential diagnosis of AE from liver neoplasias, CE from cystic liver lesions, and screening for AE in Switzerland were around 95, 86 and 2.2%, respectively. Cross-reactions with antibodies in sera of patients with other parasitic affections (fasciolosis, schistosomosis, amebosis, cysticercosis, and filarioses) did occur at variable frequencies, but could be eliminated through the use of confirmatory testing. CONCLUSIONS: Different metacestode-derived antigens of E. granulosus and E. multilocularis are valuable, widely accessible, and cost-efficient tools for the serological diagnosis of echinococcosis. However, confirmatory testing is necessary, due to the lack of species specificity and the occurrence of cross-reactions to other helminthic diseases. Abstract Purpose Human alveolar (AE) and cystic echinococcosis (CE) caused by the metacestode stages of Echinococcus multilocularis and E. granulosus, respectively, lack pathognomonic clinical signs. Diagnosis therefore relies on the results of imaging and serological studies. The primary goal of this study was to evaluate the efficacy of several easy-to-produce crude or partially purified E. granulosus and E. multilocularis metacestode-derived antigens as tools for the serological diagnosis and differential diagnosis of patients suspicious for AE or CE. Methods The sera of 51 treatment-naïve AE and 32 CE patients, 98 Swiss blood donors and 38 patients who were initially suspicious for echinococcosis but suffering from various other liver diseases (e.g., liver neoplasia, etc.) were analysed. Results According to the results of enzyme-linked immunosorbent assays (ELISA), metacestode-derived antigens of E. granulosus had sensitivities varying from 81 to 97% and [99.9% for the diagnosis of CE and AE, respectively. Antigens derived from...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Serological diagnosis of echinococcosis: the diagnostic potential of native antigens

et al. 2011

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“…Data in the literature for sensitivity and cross-reactivity of serum anti-Echinococcus IgE differ significantly; most studies report high specificity (99-100%) (Marinova et al, 2011;Chamekh et al, 1992;Khabiri et al, 2006;Afferni et al, 1984;Sjolander et al, 1989;Zarzosa et al, 1999) A recent study using a large panel of sera showed that crude or purified antigens from parasite or hydatid cyst fluid generated a reasonable high specificity (Feng et al, 2010). By using 857 sera from confirmed CE patients and 42 sera from AE patients and 697 sera with different infection and medical conditions showed an overall of 93.4% of specificity with relative low sensitivity 57.4-68.4% (Table 1) (Feng et al, 2010).…”

Section: Immunodiagnosis Of Cystic Echinococcosis In Humansmentioning

confidence: 99%

“…By using 857 sera from confirmed CE patients and 42 sera from AE patients and 697 sera with different infection and medical conditions showed an overall of 93.4% of specificity with relative low sensitivity 57.4-68.4% (Table 1) (Feng et al, 2010). …”

Section: Immunodiagnosis Of Cystic Echinococcosis In Humansmentioning

confidence: 99%