Dot-enzyme linked immunosorbent assay for the detection of antibodies in bovine brucellosis

Batra, Harsh Vardhan; Chand, Puran; Ganju, Lilly; Mukherjee, Rama; Sadana, J. R.

doi:10.1016/s0034-5288(18)31136-6

Cited by 15 publications

(8 citation statements)

References 13 publications

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“…Standard tube agglutination test (STAT) quantifies total agglutinating antibodies and higher detection rates had been reported through STAT in sheep and goat [ 12 ]. Recently, Enzyme-linked immunosorbent assay (ELISA) has taken over as an important serological tool in the diagnosis of brucellosis because of its economy, sensitivity, specificity, rapidity, reproducibility, and easy interpretation through colorimetric end product [ 13 ]. Enzyme immunoassays are superior to all other tests in terms of specificity and sensitivity [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence and comparison of different serological tests for brucellosis detection in small ruminants

Sadhu¹,

Panchasara²,

Chauhan³

et al. 2015

Vet World

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Aim:The aim was to study the seroprevalence and efficacy of the different serological tests used for detection of antibody against Brucella species in small ruminants of Banaskantha district of North-Gujarat.Materials and Methods:Total 1000 serum samples comprising of 485 from sheep and 515 from goat tested for detection of antibodies against the Brucella species by three different serological tests viz., Rose bengal plate test (RBPT), Standard tube agglutination test (STAT), and Indirect Enzyme-linked immunosorbent assay (I-ELISA).Results:The seroprevalence of brucellosis in small ruminants was 11.30%, 11.10%, and 8.80% by RBPT, STAT, and I-ELISA, respectively. The seroprevalence of brucellosis was found to be higher in sheep than goats. The sensitivity of RBPT was found slight more than STAT, but the specificity of both tests was same. In this study, the overall agreement of RBPT and STAT with I-ELISA was found 92.50% and 92.30% in small ruminants, respectively.Conclusion:I-ELISA was a better serological test as compared to RBPT and STAT in the sense of sensitivity, specificity, and rapidity and it could be advocated for screening of brucellosis in sheep and goats.

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Section: Introductionmentioning

confidence: 99%

Seroprevalence and comparison of different serological tests for brucellosis detection in small ruminants

Sadhu¹,

Panchasara²,

Chauhan³

et al. 2015

Vet World

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“…The diagnosis of the disease can be challenging and is frequently delayed or missed because the clinical picture may mimic other infectious and non-infectious conditions [3]. Recently, ELISA has taken over as an important serological tool in the diagnosis of brucellosis because of its economy, sensitivity, specificity, rapidity, reproducibility, and easy interpretation through colorimetric end product [4]. Further, the advent of milk based I-ELISA (Milk-ELISA) brings revolution in screening of large population.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and risk factor's analysis of bovine brucellosis in peri-urban areas under intensive system of production in Gujarat, India

Patel¹,

Patel²,

Prajapati³

et al. 2014

Vet World

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“…Isolation of both B. abortus and B. melitensis from horses [26] and B. abortus bv. I and III [27], bv IV [28], and bv. I [29] from cattle has been reported.…”

Section: Discussionmentioning

confidence: 99%

Genotyping of Indian antigenic, vaccine, and field Brucella spp. using multilocus sequence typing

Shome

Natesan

Shankaranarayana

et al. 2016

J Infect Dev Ctries

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Introduction: Brucellosis is one of the most important zoonotic diseases that affects multiple livestock species and causes great economic losses. The highly conserved genomes of Brucella, with > 90% homology among species, makes it important to study the genetic diversity circulating in the country. Methodology: A total of 26 Brucella spp. (4 reference strains and 22 field isolates) and 1 B. melitensis draft genome sequence from India (B. melitensis Bm IND1) were included for sequence typing. The field isolates were identified by biochemical tests and confirmed by both conventional and quantitative polymerase chain reaction (qPCR) targeting bcsp 31Brucella genus-specific marker. Brucella speciation and biotyping was done by Bruce ladder, probe qPCR, and AMOS PCRs, respectively, and genotyping was done by multilocus sequence typing (MLST). Results: The MLST typing of 27 Brucella spp. revealed five distinct sequence types (STs); the B. abortus S99 reference strain and 21 B. abortus field isolates belonged to ST1. On the other hand, the vaccine strain B. abortus S19 was genotyped as ST5. Similarly, B. melitensis 16M reference strain and one B. melitensis field isolate were grouped into ST7. Another B. melitensis field isolate belonged to ST8 (draft genome sequence from India), and only B. suis 1330 reference strain was found to be ST14. Conclusion: The sequences revealed genetic similarity of the Indian strains to the global reference and field strains. The study highlights the usefulness of MLST for typing of field isolates and validation of reference strains used for diagnosis and vaccination against brucellosis.

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Dot-enzyme linked immunosorbent assay for the detection of antibodies in bovine brucellosis

Cited by 15 publications

References 13 publications

Seroprevalence and comparison of different serological tests for brucellosis detection in small ruminants

Seroprevalence and comparison of different serological tests for brucellosis detection in small ruminants

Prevalence and risk factor's analysis of bovine brucellosis in peri-urban areas under intensive system of production in Gujarat, India

Genotyping of Indian antigenic, vaccine, and field Brucella spp. using multilocus sequence typing

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