Dot enzyme-linked immunosorbent assay (Dot-ELISA): a micro technique for the rapid diagnosis of visceral leishmaniasis

Pappas, Michael G.; Hajkowski, Ruta; Hockmeyer, Wayne T.

doi:10.1016/0022-1759(83)90399-x

Cited by 130 publications

(61 citation statements)

References 18 publications

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“…The sensitivity of Dot-ELISA obtained using HCF (93.33%), HPR (86.66%) and HCW (96.66%) antigens separately in the present study is in agreement with the results of PAPPAS et al 11 . In their study, Dot-ELISA using sheep HCF antigens showed a sensitivity of 96% and specificity of 98%.…”

Section: Discussionsupporting

confidence: 93%

Dot-Elisa for evaluation of hydatid cyst wall, protoscoleces and hydatid cyst fluid antigens in the serodiagnosis of cystic echinococcosis

Swarna

Parija

2008

Rev. Inst. Med. trop. S. Paulo

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SUMMARYThe aim of the present study is to evaluate cyst wall and protoscolex as an alternate source of antigen in serodiagnosis of cystic echinococcosis (CE). A total of 90 blood samples, 30 each of confirmed CE cases, disease controls and healthy controls were collected. Dot-ELISA using cyst wall, protoscolex and cyst fluid were used to demonstrate anti-hydatid antibodies. The sensitivity of Dot-ELISA using cyst wall, protoscolex and cyst fluid was 96.66%, 86.66% and 93.33% respectively and the specificity of the assay was 70% for Dot-ELISA using cyst fluid, protoscolex and cyst wall antigens. Results of the present study show that cyst wall and protoscolex can also be an useful source of antigen in detection of hydatid antibodies in the serodiagnosis of CE.

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Section: Discussionsupporting

confidence: 93%

Dot-Elisa for evaluation of hydatid cyst wall, protoscoleces and hydatid cyst fluid antigens in the serodiagnosis of cystic echinococcosis

Swarna

Parija

2008

Rev. Inst. Med. trop. S. Paulo

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“…The rK39 result was considered to represent a false positive caused by cross-reacting antibodies, which have been reported for this test and other serological assays for leishmaniasis in specimens from patients with rheumatological conditions. 18,19 Five bone marrow slides from four patients were available for microscopic examination and molecular testing (Table 4). Amastigotes were visualized on all five slides, and four yielded positive results by at least one molecular assay.…”

Section: Resultsmentioning

confidence: 99%

Endemic Transmission of Visceral Leishmaniasis in Bhutan

Yangzom

Cruz²,

Bern³

et al. 2012

The American Society of Tropical Medicine and Hygiene

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Abstract. Visceral leishmaniasis was first reported in Bhutan in 2006. We conducted studies of the parasite, possible vectors and reservoirs, and leishmanin skin test and risk factor surveys in three villages. Nineteen cases were reported from seven districts. Parasite typing yielded two novel microsatellite sequences, both related to Indian L. donovani. In one case village, 40 (18.5%) of 216 participants had positive leishmanin skin test results, compared with 3 (4.2%) of 72 in the other case village and 0 of 108 in the control village. Positive results were strongly associated with the village and increasing age. None of the tested dogs were infected. Eighteen sand flies were collected, 13 Phlebotomus species and 5 Sergentomyia species; polymerase chain reaction for leishmanial DNA was negative. This assessment suggests that endemic visceral leishmaniasis transmission has occurred in diverse locations in Bhutan. Surveillance, case investigations, and further parasite, vector, and reservoir studies are needed. The potential protective impact of bed nets should be evaluated.

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“…By two step alcoholic precipitation technique Dixit et al (2003) and Varghese et al (2012) Indirect plate and dot-ELISA of animals belonging to different groups: To test the diagnostic potentiality of the antigens indirect plate and dot-ELISA were standardized, since indirect plate-ELISA has been found to be very suitable for the diagnosis of parasitosis owing to its high sensitivity and possibility of processing many sera samples simultaneously. In the cases of diagnosis of parasitic infection in ruminants, it should comply the recommendation issued by the Office International des Epizooties (OIE), in the sense that it is necessary to develop diagnostic methods for parasitic diseases, which should be cheap, simple and useful under field conditions, for the reason, dot-ELISA was further standardized (Pappas et al, 1983) (Fig. 6).…”

Section: Polypeptide Profile Of Antigensmentioning

confidence: 99%

“…Thus, immunological diagnosis especially by indirect enzyme linked immunosorbent assay (ELISA) (plate and paper based) can prove to be an important tool for early diagnosis of amphistomosis which is essential for prompt treatment before irreparable damage to the GI tract (Díaz et al, 2006;Saifullah et al, 2013). The paper based ELISA can stand as pen side diagnostic test for field condition or in the laboratories with limited resources (Pappas et al, 1983). The potential of immunodiagnostic assays based on whole/ semi-purified crude antigens for early detection of helminth infections is marred by low specificity and cross-reactions due to presence of common antigenic epitopes in several trematodes (Meshgi et al, 2009;Kaur et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Immunodiagnostic potency of homologous antigens for natural Paramphistomum epiclitum infection in small ruminants in plate and paper enzyme linked immunosorbent assay

Kumar

Jadav

Das

et al. 2017

IJAR

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The objective of the present work was to standardize and evaluate indirect plate and dot-enzyme linked immunosorbent assay (ELISA) using purified Paramphistomum epiclitum homologous antigens in the small ruminants. Electrophoretic separation of somatic antigen (PeSAg) in reducing condition on 15% polyacrylamide gel resolved into 16 proteins of the molecular weight ranging from 14 -100 kDa. Two step ethanolic precipitation of supernatant of in-vitro culture of the fluke yielded P. epiclitum excretory-secretory antigen (PeESAg) of molecular weight 28 kDa. The animals (Goats=123; Sheep=91) were broadly kept into post-mortem and faecal examined groups. At many occasion the PeSAg found to cross reacts with other helminths parasites thus minimizing the specificity of the tests and antigens. There was no any direct correlation between the parasites load and ELISA reactivity pattern. The noted prevalence rate after combining the results of postmortem examination and PeESAg based ELISA (plate and paper/ dot) was 30.08% (37/123) in goats and 28.57% (26/91) in sheep. While using PeESAg, the calculated overall sensitivity% was 92.86 (goats)/ 100 (sheep) in both plate and dot-ELISA, specificity% was 91.58 (goats)/ 91.55 (sheep) in plate ELISA while 88.42 (goats)/ 92.96 (sheep) in dot-ELISA, positive predictive value% was 76.47 (goats)/ 76.92 (sheep) in plate ELISA while 70.27 (goats)/ 80 (sheep) in dot-ELISA and negative predictive value% was 97.75 (goats)/ 100 (sheep) in plate ELISA while 97.67 (goats)/ 100 (sheep) in dot-ELISA, these values were optimum for the field sera sample so the tests and PeESAg can be recommended for the detection P. epiclitum infection in the small ruminants.

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Dot enzyme-linked immunosorbent assay (Dot-ELISA): a micro technique for the rapid diagnosis of visceral leishmaniasis

Cited by 130 publications

References 18 publications

Dot-Elisa for evaluation of hydatid cyst wall, protoscoleces and hydatid cyst fluid antigens in the serodiagnosis of cystic echinococcosis

Dot-Elisa for evaluation of hydatid cyst wall, protoscoleces and hydatid cyst fluid antigens in the serodiagnosis of cystic echinococcosis

Endemic Transmission of Visceral Leishmaniasis in Bhutan

Immunodiagnostic potency of homologous antigens for natural Paramphistomum epiclitum infection in small ruminants in plate and paper enzyme linked immunosorbent assay

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