1994
DOI: 10.1590/s0036-46651994000600010
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Abstract: A dot-enzyme-linked immunosorbent assay (Dot-ELISA) for pneumococcal antigen detection was standardized in view of the need for a rapid and accurate immunodiagnosis of acute pneumococcal pneumonia. A total of 442 pleural fluid effusion samples (PFES) from children with clinical and laboratory diagnoses of acute bacterial pneumonia, plus 38 control PFES from tuberculosis patients and 20 negative control serum samples from healthy children were evaluated by Dot-ELISA. The samples were previously treated with 0.1… Show more

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Cited by 9 publications
(16 citation statements)
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“…Our proposed Dot-ELISA proved to be a practical alternative procedure for bacterial antigen detection, in view of its execution without the need for reading equipment, and economy of pneumococcal omniserum and other bacterial antiserum, that may be used at 1:200 dilution [6][7][8]. Positivities obtained by Dot-ELISA are higher than that obtained by CIE and LA, when pleural fluid, serum, and/or urine samples are employed in the tests, despite the similar values of sensitivity.…”
Section: Discussionmentioning
confidence: 99%
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“…Our proposed Dot-ELISA proved to be a practical alternative procedure for bacterial antigen detection, in view of its execution without the need for reading equipment, and economy of pneumococcal omniserum and other bacterial antiserum, that may be used at 1:200 dilution [6][7][8]. Positivities obtained by Dot-ELISA are higher than that obtained by CIE and LA, when pleural fluid, serum, and/or urine samples are employed in the tests, despite the similar values of sensitivity.…”
Section: Discussionmentioning
confidence: 99%
“…Paired samples of pleural fluid, serum and urine from 550 children with clinical diagnosis of bacterial pneumonia were assayed by counterimmunoelectrophoresis (CIE), latex agglutination (LA), and dot-enzyme-linked immunosorbent assay (Dot-ELISA) for antigen detection according to previously standardized procedures [4][5][6][7][8]. Anti-Streptococcus pneumoniae omniserum against 90 serotypes (pneumococcal omniserum) from Seruminstitut, Copenhagen, Denmark [9] and anti-Haemophilus influenzae type b produced according to routine procedure [10] were employed in the immunological methods.…”
Section: Methodsmentioning
confidence: 99%
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“…ELISA-based rapid tests allow a diagnosis of pneumococcal pneumonia to be made with a sensitivity of >70% by the detection of capsular antigen in serum [15] or pleural fluid [16]. However, this method is not widely used and is not yet commercially available.…”
Section: Elisamentioning
confidence: 99%