Dot-blot immunoassay for detection of tomato chlorosis virus and reaction of potato genotypes to virus infection

Upconversion Nanoparticle-Based Dot-Blot Immunoassay for Quantitative Biomarker Detection

“…Dot-blot is a simple immunoassay technique in which proteins are directly deposited on the membrane surface and adsorbed through noncovalent interactions . This user-friendly method does not require expensive instrumentation and can be applied for rapid PoC analysis of analytes such as infectious agents, clinically relevant antibodies, and biomarkers. − Many types of labels have been used for signal generation in dot-blots, mainly enzymes , and fluorophores . However, these conventional labels typically have some drawbacks, e.g., the limited stability of enzymes or photobleaching in the case of fluorophores.…”

Virotest: a bioinformatics pipeline for virus identification in plants

“…However, it lacks the resolution to properly identify some viral strains. On the contrary, molecular probes based on nucleic acids, such as dot-blot, tissue print, polymerase chain reaction (PCR), real-time quantitative PCR (qPCR) or isothermal PCR, can be generated at low cost and have the required flexibility to deal with the distinction of viral sequence variants that do not rely on amino-acid changes [14]- [17]. Multiplexing and high-throughput techniques have been also applied to virus detection, such as microarrays, but some limitations persist related to cross-hybridization and the lack of capabilities to discover uncharacterized viruses.…”