DOsimetry of BIological EXperiments in SPace (DOBIES) with luminescence (OSL and TL) and track etch detectors

Vanhavere, F.; Genicot, J.-L.; O’Sullivan, D.; Zhou, D.; Spurný, F.; Jadrníčková, I.; Sawakuchi, Gabriel O.; Yukihara, E.G.

doi:10.1016/j.radmeas.2007.12.002

Cited by 32 publications

(25 citation statements)

References 6 publications

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“…0.16 mGy day À1 of g rays and 0.02 mGy day À1 of neutron rays, totaling ca. 1.8 mGy per 10 days, which is comparable with the actual space flights experiments (Goossens et al, 2006;Vanhavere et al 2008 (Bradford, 1976), according to the Bio-Rad Protein Assay kit (Bio-Rad, Hercules, CA, USA), with bovine g-globulin as a protein standard. Protein concentration of each sample was adjusted to 5 mg ml À1 and equal amounts of the three biological replicates were pooled before labeling.…”

Section: Methodssupporting

confidence: 61%

“…Radiation dosimeters (Track-Etch Detectors and Optically Stimulated Luminescence Detectors described in Vanhavere et al, 2008) and temperature sensors (SmartButton, ACR Systems, Surrey, BC, Canada) were added in the container. Two culture plates were sealed hermetically in one polycarbonate 'Biocontainer' (PedeoTechniek, Oudenaarde, Belgium) (described in Vanhavere et al, 2008) and vacuum sealed in a highly transparent Minigrip polyethylene bag (Minigrip, Brussels, Belgium) of 60-mm thickness. During the 2-day trip in the Soyuz (TMA-9) to the ISS, the temperature of the pouch was maintained at 22 ± 1 1C.…”

Section: Methodsmentioning

confidence: 99%

“…400 km altitude) indicated an average dose of ca. 180mGy day À1 (Goossens et al, 2006;Vanhavere et al, 2008). Thus, on board the ISS, organisms undergo radiation stress up to 80-fold higher than the background radiation levels on the Earth.…”

Section: Introductionmentioning

confidence: 99%

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Experimental design and environmental parameters affect Rhodospirillum rubrum S1H response to space flight

et al. 2009

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In view of long-haul space exploration missions, the European Space Agency initiated the MicroEcological Life Support System Alternative (MELiSSA) project targeting the total recycling of organic waste produced by the astronauts into oxygen, water and food using a loop of bacterial and higher plant bioreactors. In that purpose, the a-proteobacterium, Rhodospirillum rubrum S1H, was sent twice to the International Space Station and was analyzed post-flight using a newly developed R. rubrum whole genome oligonucleotide microarray and high throughput gel-free proteomics with Isotope-Coded Protein Label technology. Moreover, in an effort to identify a specific response of R. rubrum S1H to space flight, simulation of microgravity and space-ionizing radiation were performed on Earth under identical culture set-up and growth conditions as encountered during the actual space journeys. Transcriptomic and proteomic data were integrated and permitted to put forward the importance of medium composition and culture set-up on the response of the bacterium to space flight-related environmental conditions. In addition, we showed for the first time that a low dose of ionizing radiation (2 mGy) can induce a significant response at the transcriptomic level, although no change in cell viability and only a few significant differentially expressed proteins were observed. From the MELiSSA perspective, we could argue the effect of microgravity to be minimized, whereas R. rubrum S1H could be more sensitive to ionizing radiation during long-term space exploration mission.

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Section: Methodssupporting

confidence: 61%

Section: Methodsmentioning

confidence: 99%

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Experimental design and environmental parameters affect Rhodospirillum rubrum S1H response to space flight

et al. 2009

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“…3.4). Passive detector systems have been used since the Mercury missions (Warren & Gill 1964) and especially the combination of luminescence detectors with nuclear track etch detectors is currently widely applied onboard the ISS because it allows not only the determination of the absorbed dose, but also the dose equivalent in combining these two detector systems (see for example: Vanhavere et al 2008). Active silicon detector systems are commonly used onboard the ISS (see also Narici et al 2015), and therefore offer good baseline data for intercomparison purposes.…”

Section: Detector Systemsmentioning

confidence: 99%

DOSIS & DOSIS 3D: long-term dose monitoring onboard the Columbus Laboratory of the International Space Station (ISS)

Berger

Przybyla

Matthiä

et al. 2016

J. Space Weather Space Clim.

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The radiation environment encountered in space differs in nature from that on Earth, consisting mostly of highly energetic ions from protons up to iron, resulting in radiation levels far exceeding the ones present on Earth for occupational radiation workers. Since the beginning of the space era, the radiation exposure during space missions has been monitored with various active and passive radiation instruments. Also onboard the International Space Station (ISS), a number of area monitoring devices provide data related to the spatial and temporal variation of the radiation field in and outside the ISS. The aim of the DOSIS (2009DOSIS ( -2011 and the DOSIS 3D (2012-ongoing) experiments was and is to measure the radiation environment within the European Columbus Laboratory of the ISS. These measurements are, on the one hand, performed with passive radiation detectors mounted at 11 locations within Columbus for the determination of the spatial distribution of the radiation field parameters and, on the other, with two active radiation detectors mounted at a fixed position inside Columbus for the determination of the temporal variation of the radiation field parameters. Data measured with passive radiation detectors showed that the absorbed dose values inside the Columbus Laboratory follow a pattern, based on the local shielding configuration of the radiation detectors, with minimum dose values observed in the year 2010 of 195-270 lGy/day and maximum values observed in the year 2012 with values ranging from 260 to 360 lGy/day. The absorbed dose is modulated by (a) the variation in solar activity and (b) the changes in ISS altitude.

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“…From that point on, the same procedures as for the BASE-A experiment were followed as described previously (Leys et al, 2009). Temperature and radiation were logged as described previously (Goossens et al, 2006;Vanhavere et al, 2008).…”

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confidence: 99%

The Influence of Microgravity on Invasive Growth inSaccharomyces cerevisiae

et al. 2011

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This study investigates the effects of microgravity on colony growth and the morphological transition from single cells to short invasive filaments in the model eukaryotic organism Saccharomyces cerevisiae. Two-dimensional spreading of the yeast colonies grown on semi-solid agar medium was reduced under microgravity in the S1278b laboratory strain but not in the CMBSESA1 industrial strain. This was supported by the S1278b proteome map under microgravity conditions, which revealed upregulation of proteins linked to anaerobic conditions. The S1278b strain showed a reduced invasive growth in the center of the yeast colony. Bud scar distribution was slightly affected, with a switch toward more random budding. Together, microgravity conditions disturb spatially programmed budding patterns and generate strain-dependent growth differences in yeast colonies on semi-solid medium.

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DOsimetry of BIological EXperiments in SPace (DOBIES) with luminescence (OSL and TL) and track etch detectors

Cited by 32 publications

References 6 publications

Experimental design and environmental parameters affect Rhodospirillum rubrum S1H response to space flight

Experimental design and environmental parameters affect Rhodospirillum rubrum S1H response to space flight

DOSIS & DOSIS 3D: long-term dose monitoring onboard the Columbus Laboratory of the International Space Station (ISS)

The Influence of Microgravity on Invasive Growth inSaccharomyces cerevisiae

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