Doses effects of zoledronic acid on mineral apatite and collagen quality of newly-formed bone in the rat's calvaria defect

Olejnik, Cécile; Falgayrac, Guillaume; During, Alexandrine; Cortet, Bernard; Penel, Guillaume

doi:10.1016/j.bone.2016.05.002

Cited by 22 publications

(10 citation statements)

References 70 publications

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“…As both proline and hydroxyproline are often considered together, due to their prevalence in the X and Y position of the glycine-X-Y repeat of type I collagen ( Fig. 1 C) [ 28 , 29 ], the sum of intensities significantly increased on PLL coated quartz versus uncoated controls as expected ( Fig. 2 D; +2.23 and +4.26-fold respectively).…”

Section: Resultssupporting

confidence: 64%

Raman spectroscopy links differentiating osteoblast matrix signatures to pro-angiogenic potential

Sharma

Goring

Staines

et al. 2020

Matrix Biology Plus

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Mineralization of bone is achieved by the sequential maturation of the immature amorphous calcium phase to mature hydroxyapatite (HA) and is central in the process of bone development and repair. To study normal and dysregulated mineralization in vitro, substrates are often coated with poly- l -lysine (PLL) which facilitates cell attachment. This study has used Raman spectroscopy to investigate the effect of PLL coating on osteoblast (OB) matrix composition during differentiation, with a focus on collagen specific proline and hydroxyproline and precursors of HA. Deconvolution analysis of murine derived long bone OB Raman spectra revealed collagen species were 4.01-fold higher in OBs grown on PLL. Further, an increase of 1.91-fold in immature mineral species (amorphous calcium phosphate) was coupled with a 9.32-fold reduction in mature mineral species (carbonated apatite) on PLL versus controls. These unique low mineral signatures identified in OBs were linked with reduced alkaline phosphatase enzymatic activity, reduced Alizarin Red staining and altered osteogenic gene expression. The promotion of immature mineral species and restriction of mature mineral species of OB grown on PLL were linked to increased cell viability and pro-angiogenic vascular endothelial growth factor (VEGF) production. These results demonstrate the utility of Raman spectroscopy to link distinct matrix signatures with OB maturation and VEGF release. Importantly, Raman spectroscopy could provide a label-free approach to clinically assess the angiogenic potential of bone during fracture repair or degenerative bone loss.

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Section: Resultssupporting

confidence: 64%

Raman spectroscopy links differentiating osteoblast matrix signatures to pro-angiogenic potential

Sharma

Goring

Staines

et al. 2020

Matrix Biology Plus

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“…2 and 3), the intensities of Hyp and Pro peaks were recently reported to increase with in vitro ribose and glucose incubation of type I collagen from rat tail tendons (59). The Hyp/Pro ratio was previously suggested as an indicator of PTMs of collagen I (45, 60) due to the enzymatic processing of proline (the hydroxylation of the proline residue). However, in vitro incubation of cadaveric bone in sugar is not expected to induce any hydroxylation processing of proline, but rather NEG targets lysine and arginine residues of collagen.…”

Section: Discussionmentioning

confidence: 99%

Assessing glycation‐mediated changes in human cortical bone with Raman spectroscopy

Ünal

Uppuganti

Leverant

et al. 2018

Journal of Biophotonics

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Establishing a non-destructive method for spatially assessing advanced glycation end-products (AGEs) is a potentially useful step toward investigating the mechanistic role of AGEs in bone quality. To test the hypothesis that the shape of the amide I in the Raman spectroscopy (RS) analysis of bone matrix changes upon AGE accumulation, we incubated paired cadaveric cortical bone in ribose or glucose solutions and in control solutions for 4 and 16 weeks, respectively, at 37°C. Acquiring 10 spectra per bone with a 20X objective and a 830 nm laser, RS was sensitive to AGE accumulation (confirmed by biochemical measurements of pentosidine and fluorescent AGEs). Hyp/Pro ratio increased upon glycation using either 0.1 M ribose, 0.5 M ribose or 0.5 M glucose. Glycation also decreased the amide I sub-peak ratios (cm ) 1668/1638 and 1668/1610 when directly calculated using either second derivative spectrum or local maxima of difference spectrum, though the processing method (eg, averaged spectrum vs individual spectra) to minimize noise influenced detection of differences for the ribose-incubated bones. Glycation however did not affect these sub-peak ratios including the matrix maturity ratio (1668/1690) when calculated using indirect sub-band fitting. The amide I sub-peak ratios likely reflected changes in the collagen I structure.

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“…The hydroxyproline-to-proline ratio was calculated as the intensity ratio of hydroxyproline (877 cm −1 ) and proline (853 cm −1 ). The study of the hydroxyproline and proline Raman bands was shown to give a reliable evaluation of post-translational modifications of collagen in bone 22, 46, 47 . This ratio provides an assessment of the collagen secondary structure with implications for bone mineralization capability 48 .…”

Section: Methodsmentioning

confidence: 99%

Region specific Raman spectroscopy analysis of the femoral head reveals that trabecular bone is unlikely to contribute to non-traumatic osteonecrosis

Pascart

Falgayrac

Migaud

et al. 2017

Sci Rep

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Non-traumatic osteonecrosis (ON) of the femoral head is a common disease affecting a young population as the peak age of diagnosis is in the 40 s. The natural history of non-traumatic ON leads to a collapse of the femoral head requiring prosthetic replacement in a 60% of cases. Although trabecular bone involvement in the collapse is suspected, the underlying modifications induced at a molecular level have not been explored in humans. Here, we examine changes in the molecular composition and structure of bone as evaluated by Raman spectroscopy in human end-stage ON. Comparing samples from femoral heads harvested from 11 patients and 11 cadaveric controls, we show that the mineral and organic chemical composition of trabecular bone in ON is not modified apart from age-related differences. We also show that the molecular composition in the necrotic part of the femoral head is not different from the composition of the remaining ‘healthy’ trabecular bone of the femoral head. These findings support that quality of trabecular bone is not modified during ON despite extensive bone marrow necrosis and osteocyte death observed even in the ‘healthy’ zones on histological examination.

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Doses effects of zoledronic acid on mineral apatite and collagen quality of newly-formed bone in the rat's calvaria defect

Cited by 22 publications

References 70 publications

Raman spectroscopy links differentiating osteoblast matrix signatures to pro-angiogenic potential

Raman spectroscopy links differentiating osteoblast matrix signatures to pro-angiogenic potential

Assessing glycation‐mediated changes in human cortical bone with Raman spectroscopy

Region specific Raman spectroscopy analysis of the femoral head reveals that trabecular bone is unlikely to contribute to non-traumatic osteonecrosis

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