Dose-Sparing Intradermal DTaP-sIPV Immunization With a Hollow Microneedle Leads to Superior Immune Responses

Abstract

Dose-sparing intradermal (ID) vaccination may induce the same immune responses as intramuscular (IM) vaccination, which can increase vaccine supplies and save costs. In this study, rats were immunized with fractional-dose of Sabin-derived IPV combined with diphtheria-tetanus-acellular pertussis vaccine (DTaP-sIPV) intradermally with hollow microneedle devices called MicronJet600 and the vaccine immunogenicity and efficacy were evaluated and compared with those of full-dose intramuscular immunization. We tested levels of antibodies and the subclass distribution achieved via different immunization routes. Furthermore, gene transcription in the lung and spleen, cytokine levels and protection against Bordetella pertussis (B. pertussis) infection were also examined. The humoral immune effect of DTaP-sIPV delivered with MicronJet600 revealed that this approach had a significant dose-sparing effect and induced more effective protection against B. pertussis infection by causing Th1/Th17 responses. In conclusion, ID immunization of DTaP-sIPV with the MicronJet600 is a better choice than IM immunization, and it has the potential to be a new DTaP-sIPV vaccination strategy.

Exploiting Unique Features of Microneedles to Modulate Immunity

“…im-munization improved protection against the B. Pertussis bacteria, as measured by the number of colony-forming units in various organs. [165] Despite these lower systemic levels, delivery of vaccine components via MNAs can achieve similar or higher antigen-specific antibody titers compared to dose-matched delivery through conventional routes. In one recent study, mice were immunized against a virus (Polio) and bacteria (Tetanus, Diphtheria, and Pertussis) using a combination vaccine.…”

“…immunization improved protection against the B. Pertussis bacteria, as measured by the number of colony‐forming units in various organs. [ 165 ]…”

“…MNA treatment also activated B cells to produce significantly more antibodies against all implicated pathogens. [ 165 ] This study is particularly interesting for a few reasons. First, MNAs caused a distant response in organs required to mount a response against the disease.…”

Exploiting Unique Features of Microneedles to Modulate Immunity

“…For example, hollow MNAs delivering a fifth of a full-dose I.M. immunization improved protection against the B. Pertussis bacteria, as measured by the number of colony-forming units in various organs 166 .…”

Smart Physical‐Based Transdermal Drug Delivery System:Towards Intelligence and Controlled Release

“…[ 54 ] It had good drug loading and controllability, and better drug delivery ability, which was different from other microneedle systems described above. [ 55 ] Hollow microneedle also had better hardness than other microneedle systems, and was typically made from metal, silicon, ceramic or carbon materials through complex techniques such as etching, laser micromachining, microelectromechanical systems (MEMS) technology and 3D printing, which is a recent research hotspot. Niu et al developed a hollow microneedle to protect the human body by subcutaneously delivering polymer nanoparticles containing ovalbumin to induce an enhanced immune response.…”