Dose-Response Relationships between Exogenous Progesterone and Estradiol and Porcine Uterine Protein Secretions

Knight, James; Bazer, Fuller W.; Wallace, H. D.; Wilcox, C.J.

doi:10.2527/jas1974.394747x

Cited by 42 publications

(20 citation statements)

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“…These studies further suggested that histotroph production increased primarily in response to ovarian progesterone during pregnancy in the pig (Knight et al 1974a, 1974b, Schlosnagle et al 1974, Basha et al 1980, Geisert et al 1982a, 1982b. Indeed, the idea that progesterone alone, in the absence of ovarian or placental factors, is sufficient to support pregnancy levels of histotroph is supported by data showing that amounts of luminal secretory protein recovered per horn from ovariectomized gilts administered progesterone daily for up to 60 days are comparable to those obtained from pseudopregnant and unilaterally pregnant animals (Schlosnagle et al 1974, Basha et al 1980.…”

Section: Progesterone Effects On Development Of Pig Gementioning

confidence: 90%

“…Although similar gene expression studies have not been performed in pigs, progesterone increases the expression of calbindin-D9k (Yun et al 2004), vascular endothelial growth factor (Welter et al 2004), fibroblast growth factors (FGFs) 2 and 7, two FGF receptors, FGFR1 and FGFR2 (Welter et al 2003, a 4 , a 5 , and b 1 integrin receptor subunits (Bowen et al 1996), the swine leukocyte antigens 1, 2, and 3, and b(2)-microglobulin (Joyce et al 2008), as well as suppresses expression of MUC1 and progesterone receptor in luminal epithelium (LE; Geisert et al 1994, Bowen et al 1996. Importantly, progesterone increases the expression of various uterine secretory proteins that are components of the histotroph, which is hypothesized to support conceptus and fetal development in pigs (Knight et al 1974a, 1974b, Roberts & Bazer 1988.…”

Section: Introductionmentioning

confidence: 99%

“…Multiple studies suggest that histotroph production increases primarily in response to ovarian progesterone during pregnancy in the pig (Knight et al 1974a, 1974b, Schlosnagle et al 1974, Basha et al 1980, Geisert et al 1982a, 1982b, and that progesterone alone is sufficient to support pregnancy levels of histotroph (Schlosnagle et al 1974, Basha et al 1980. However, results for uteroferrin, a well-known progesterone-induced component of histotroph, suggest that interactions among progesterone, lactogenic hormones, and ovarian steroids may constitute a 'servomechanism' regulating endometrial remodeling, secretory function, and uterine growth in pigs (Young et al 1990).…”

Section: Introductionmentioning

confidence: 99%

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Effects of long-term progesterone on developmental and functional aspects of porcine uterine epithelia and vasculature: progesterone alone does not support development of uterine glands comparable to that of pregnancy

Bailey¹,

Dunlap²,

Frank³

et al. 2010

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In pigs, endometrial functions are regulated primarily by progesterone and placental factors including estrogen. Progesterone levels are high throughout pregnancy to stimulate and maintain secretion of histotroph from uterine epithelia necessary for growth, implantation, placentation, and development of the conceptus (embryo and its extra-embryonic membranes). This study determined effects of longterm progesterone on development and histoarchitecture of endometrial luminal epithelium (LE), glandular epithelium (GE), and vasculature in pigs. Pigs were ovariectomized during diestrus (day 12), and then received daily injections of either corn oil or progesterone for 28 days. Prolonged progesterone treatment resulted in increased weight and length of the uterine horns, and thickness of the endometrium and myometrium. Hyperplasia and hypertrophy of GE were not evident, but LE cell height increased, suggesting elevated secretory activity. Although GE development was deficient, progesterone supported increased endometrial angiogenesis comparable to that of pregnancy. Progesterone also supported alterations to the apical and basolateral domains of LE and GE. Dolichos biflorus agglutinin lectin binding and a v integrin were downregulated at the apical surfaces of LE and GE. Claudin-4, a 2 b 1 integrin, and vimentin were increased at basolateral surfaces, whereas occludins-1 and -2, claudin-3, and E-cadherin were unaffected by progesterone treatment indicating structurally competent trans-epithelial adhesion and tight junctional complexes. Collectively, the results suggest that progesterone affects LE, GE, and vascular development and histoarchitecture, but in the absence of ovarian or placental factors, it does not support development of GE comparable to pregnancy. Furthermore, LE and vascular development are highly responsive to the effects of progesterone.

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Section: Progesterone Effects On Development Of Pig Gementioning

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of long-term progesterone on developmental and functional aspects of porcine uterine epithelia and vasculature: progesterone alone does not support development of uterine glands comparable to that of pregnancy

Bailey¹,

Dunlap²,

Frank³

et al. 2010

Reproduction

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“…Alternatively, the decline in acid phosphatase between Days 14 and 18 may reflect a decline in uteroferrin synthesis and/or secretion due to increasing production of oestrogen by the conceptuses. Knight, Bazer, Wallace & Wilcox (1974) and Basha, Bazer & Roberts (1979) determined that low levels of oestrogen are synergistic with progesterone in increasing uterine secretory activity, while high levels of oestrogen were inhibitory. Oestrogen concentrations in uterine flushings and maternal blood increase during the period of conceptus elongation and early placentation whereas progesterone declines (see .…”

Section: Discussionmentioning

confidence: 99%

Acid phosphatase and leucine aminopeptidase activity in the uterine flushings of non-pregnant and pregnant gilts

Zavyt¹,

Roberts²,

Bazer³

1984

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Summary. The activities of uteroferrin, measured as acid phosphatase (AP), and an aminoacylpeptidase (AA) were measured in uterine flushings collected from gilts on Days 6, 8, 10, 12, 14, 15, 16 and 18 of the oestrous cycle and pregnancy (N = 37). Changes in AP (P < 0\m=.\05) were associated with day for both specific and total AP in non-pregnant and pregnant gilts. For pregnant and non-pregnant gilts, AP activity was greatest between Days 14 and 16 and then decreased to Day 18.The AA specific activity increased (P < 0\m=.\01) between Days 10 and 12 of the oestrous cycle and pregnancy, but neither effects of pregnancy nor day by pregnancy status interaction were detected. The AA total activity was greater for pregnant gilts (P < 0\m=.\01). These data suggest an inhibitory effect of oestrogens of blastocyst origin on synthesis and/or secretion of uteroferrin, but not AA.

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“…UF was discovered and initially characterized in my laboratory [6,7]. We demonstrated that progesterone (P4) stimulates secretion of UF and that synergistic actions of estradiol (E2) and P4 increase its secretion when total E2 does not exceed 1 lg/day but that higher doses of E2 inhibit its secretion by uterine glandular (GE) epithelium in pigs [8][9][10]. We purified UF, produced polyclonal antiserum to it and used immunofluorescence histochemistry to demonstrate its secretion by uterine GE of pigs and its uptake up by the placenta [11,12].…”

Section: Uterine Secretionsmentioning

confidence: 98%

Contributions of an Animal Scientist to Reproductive Biology

Bazer

2011

Biology of Reproduction

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I became interested in biology as an undergraduate in a premedical curriculum but developed a passion for the field of reproductive biology because of a course in physiology of reproduction taken to meet requirements for admission to veterinary school. My career path changed, and I entered graduate school, obtained the Ph.D., and have enjoyed an academic career as a reproductive biologist conducting research in uterine biology and pregnancy in animal science departments at the University of Florida and at Texas A&M University. However, I have never allowed academic boundaries to interfere with research and graduate education as that is contrary to collegiality, the cornerstone of great universities. I consider that my major contributions to science include 1) identification of proteins secreted by cells of the uterine endometrium that are critical to successful establishment and maintenance of pregnancy; 2) discovery of steroids and proteins required for pregnancy recognition signaling and their mechanisms of action in pigs and ruminant species; 3) investigation of fetal-placental development and placental transport of nutrients, including water and electrolytes; 4) identification of linkages between nutrition and fetal-placental development; 5) defining aspects of the endocrinology of pregnancy; and 6) contributing to efforts to exploit the therapeutic value of interferon tau, particularly for treatment of autoimmune diseases. My current studies are focused on the role of select nutrients in the uterine lumen, specifically amino acids and glucose, that affect development and survival of the conceptus and translation of mRNAs and, with colleagues at Seoul National University, gene expression by the avian reproductive tract at key periods postovulation. Another goal is to understand stromal-epithelial cell signaling, whereby progesterone and estrogen act via uterine stromal cells that express receptors for sex steroids to stimulate secretion of growth factors (e.g., fibroblast growth factors and hepatocyte growth factor) that, in turn, regulate functions of uterine epithelial cells and conceptus trophectoderm.

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Dose-Response Relationships between Exogenous Progesterone and Estradiol and Porcine Uterine Protein Secretions

Cited by 42 publications

References 0 publications

Effects of long-term progesterone on developmental and functional aspects of porcine uterine epithelia and vasculature: progesterone alone does not support development of uterine glands comparable to that of pregnancy

Effects of long-term progesterone on developmental and functional aspects of porcine uterine epithelia and vasculature: progesterone alone does not support development of uterine glands comparable to that of pregnancy

Acid phosphatase and leucine aminopeptidase activity in the uterine flushings of non-pregnant and pregnant gilts

Contributions of an Animal Scientist to Reproductive Biology

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