Dose-response Relationship of Lymphocyte Chromosome Aberrations in Locally Irradiated Persons

Matsubara, Sho; Sasaki, Masao S.; Adachi, Takuya

doi:10.1269/jrr.15.189

Cited by 40 publications

(16 citation statements)

References 10 publications

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“…While the calculated EWBD does not take into account this parameter, the biological whole-body dose estimated from the chromosome aberrations scored in lymphocytes may inevitably be impacted. This hypothesis is supported by data from other studies indicating variations in the yield of chromosome aberrations according to the localisation of the exposure, even when corrections were made for the volume of exposed tissue [9,10,22]. Also, in cervical cancer patients treated with radiotherapy, the EWBD calculated with the same formula and the wholebody dose estimated by cytogenetic methods were quite similar [13].…”

Section: Discussionsupporting

confidence: 74%

Cytogenetic assessment of heterogeneous radiation doses in cancer patients treated with fractionated radiotherapy

Roch-Lefèvre

Pouzoulet²,

Giraudet³

et al. 2010

BJR

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ABSTRACT. The purpose of this study was to evaluate the in vivo dose-response relation of chromosome aberration formation and distribution in a context of localised and fractionated radiotherapy. Cytogenetic analysis was applied to eight patients, all treated for the same tumour localisation; the same localisation was used to prevent the variability usually observed between patients treated with radiotherapy and to allow the corresponding roles of the size of irradiation field and of the dose rate to be studied. The yield of dicentrics, centric rings and fragments was measured in blood samples taken before treatment, during the course of radiotherapy and up to 6 months after. After the first fraction of radiotherapy, we observed that the whole-body dose estimated from the yield of dicentrics and rings was higher (0.35¡0.2 Gy) than the calculated equivalent whole-body dose (0.07¡0.04 Gy). By contrast, the partial-body dose derived from the Qdr (quotient of dicentrics and rings) model was estimated to be 2.2¡0.3 Gy, which agreed quite well with the dose delivered to the tumour (2.1¡0.1 Gy). We also found a correlation between the yield of induced chromosome aberrations and the target field size (p50.014). U-value analysis showed that the distribution of dicentrics and rings was overdispersed, despite the fractionation of the exposure, and a positive correlation between the U-value and the dose rate was observed (p50.017). Overall, these results suggest that the proportion of undamaged lymphocytes could increase with the dose rate.

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Section: Discussionsupporting

confidence: 74%

Cytogenetic assessment of heterogeneous radiation doses in cancer patients treated with fractionated radiotherapy

Roch-Lefèvre

Pouzoulet²,

Giraudet³

et al. 2010

BJR

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“…The surviving fraction ( S ) was calculated using the equations reported by Lloyd and coworkers [15] and Matsubara and coworkers [16]. The frequencies of rings per cell necessary for the calculation of S were obtained from simulated whole- and partial-body irradiation.…”

Section: Methodsmentioning

confidence: 99%

Assessment of simulated high-dose partial-body irradiation by PCC-R assay

Romero

García

Lamadrid

et al. 2013

Journal of Radiation Research

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The estimation of the dose and the irradiated fraction of the body is important information in the primary medical response in case of a radiological accident. The PCC-R assay has been developed for high-dose estimations, but little attention has been given to its applicability for partial-body irradiations. In the present work we estimated the doses and the percentage of the irradiated fraction in simulated partial-body radiation exposures at high doses using the PCC-R assay. Peripheral whole blood of three healthy donors was exposed to doses from 0–20 Gy, with 60Co gamma radiation. To simulate partial body irradiations, irradiated and non-irradiated blood was mixed to obtain proportions of irradiated blood from 10–90%. Lymphocyte cultures were treated with Colcemid and Calyculin-A before harvest. Conventional and triage scores were performed for each dose, proportion of irradiated blood and donor. The Papworth's u test was used to evaluate the PCC-R distribution per cell. A dose-response relationship was fitted according to the maximum likelihood method using the frequencies of PCC-R obtained from 100% irradiated blood. The dose to the partially irradiated blood was estimated using the Contaminated Poisson method. A new D0 value of 10.9 Gy was calculated and used to estimate the initial fraction of irradiated cells. The results presented here indicate that by PCC-R it is possible to distinguish between simulated partial- and whole-body irradiations by the u-test, and to accurately estimate the dose from 10–20 Gy, and the initial fraction of irradiated cells in the interval from 10–90%.

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“…Cytogenetic techniques, including the widely used analysis of chromosome aberrations [2,8,15,26,27,31,34,37,46] and the nowadays frequently used micronucleus test [3, 7, 9, 16, 23, 25, 28, 31, 34-36, 39, 42], are not only reliable indicators of damage to the cell genome in vivo and in vitro, but are also applicable for different and combined radiation qualities [45]. Our data agree with other authors mentioned above who demonstrated that RTX causes structural chromosome damage.…”

Section: Discussionmentioning

confidence: 99%

“…Concerning the CAT, a dose-dependent increase in the yield of CA frequency has been shown in RTX of patients at different sites [2,8,26,27]. It is a sensitive method for dose estimation but requires a very detailed analysis, which is very laborious and complex.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Micronucleus and Chromosome Aberration Techniques for the Documentationof Cytogenetic Damage in Radiochemotherapy-Treated Patients with Rectal Cancer

et al. 2010

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The results show that the CBMN assay and the CAT are equivalent. For further investigations, we prefer the CBMN assay, because it is simpler through easy scoring criteria, allows high numbers of cell counts in less time, is reliable, sensitive, and has higher statistical power. In the future, we plan to integrate cytogenetic damage during radiochemotherapy into the planned Response and Toxicity Score within our interdisciplinary Clinical Research Unit.

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Dose-response Relationship of Lymphocyte Chromosome Aberrations in Locally Irradiated Persons

Cited by 40 publications

References 10 publications

Cytogenetic assessment of heterogeneous radiation doses in cancer patients treated with fractionated radiotherapy

Cytogenetic assessment of heterogeneous radiation doses in cancer patients treated with fractionated radiotherapy

Assessment of simulated high-dose partial-body irradiation by PCC-R assay

Comparison of the Micronucleus and Chromosome Aberration Techniques for the Documentationof Cytogenetic Damage in Radiochemotherapy-Treated Patients with Rectal Cancer

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