Dose−Response of Seeds of the Parasitic Weeds<i>Striga</i>and<i>Orobanche</i>toward the Synthetic Germination Stimulants GR 24 and Nijmegen 1

Wigchert, Suzanne C. M.; Kuiper, E.; Boelhouwer, G.J.; Nefkens, G. H. L.; Verkleij, J. A. C.; Zwanenburg, B.

doi:10.1021/jf981006z

Cited by 75 publications

(52 citation statements)

References 27 publications

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“…Successful surface sterilization and germination of parasite seeds is prerequisite for derivation of in vitro culture. It is well known, that Striga and Orobanche seeds germinate in response to native or synthetic (GR24 and Nijmegen 1) germination stimulants (Wigchert et al 1999). Different combinations of plant growth regulators were studied for establishment of in vitro cultures of Orobanche ramosa, O. minor, O. aegyptica (Zhou et al 2004), C. reflexa (Srivastava & Dwivedi 2001), C. japonica (Furuhashi 1991) and C. trifolli (Bakos et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Stable transformation and actin visualization in callus cultures of dodder (Cuscuta europaea)

Švubová

Blehová

2013

Biologia

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Agrobacterium tumefaciens-mediated transformation of callus culture, combined with a visual selection of GFPtagged fimbrin actin binding domain (FABD2) expression is described for parasitic species (Cuscuta europaea). The conditions for callus induction from 1 cm-long explants from the basal part of 7-day-old dodder seedlings were defined. We obtained light-green calli, which were transformed with A. tumefaciens bacterial strain GV3101 carrying plasmid pCB302 (35S::ABD2:gfp) with neomycin phosphotransferase (nptII) gene. The limitations of selection procedures based on antibiotics were avoided using green fluorescent protein (GFP) detection, as a visual selection marker subcellularly targeted to the actin cytoskeleton. Fluorescence microscopy analyses demonstrated a network of nucleus-associated actin arrays and dense cortical actin arrangements in stably transformed Cuscuta callus cells. RT-PCR analyses confirmed gfp expression in transformed calli 7, 14 and 21 days after transformation. Although the GFP fluorescence associated with the actin cytoskeleton has retained for at least six months without silencing, no shoot regeneration was observed. It can be concluded that, C. europaea callus cells are competent for transformation, but under given conditions, these cells failed to realize their morphogenic and regeneration potentials.

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Section: Introductionmentioning

confidence: 99%

Stable transformation and actin visualization in callus cultures of dodder (Cuscuta europaea)

Švubová

Blehová

2013

Biologia

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“…Sigmoidalshaped curves display a suboptimal (linear) zone at low strigolactone concentrations, reaching an optimal (saturation) zone at high concentrations. Bell-shaped curves show also a supraoptimal zone, characterized by a decline of the response at considerably high strigolactone concentrations (Wigchert et al 1999). This pattern could hold true also for GR24 inhibition of axillary buds.…”

Section: Response To Gr24mentioning

confidence: 66%

Strigolactone may interact with gibberellin to control apical dominance in pea (Pisum sativum)

2011

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The role of strigolactones as plant growth regulators has been demonstrated through research on biosynthesis and signaling mutant plants and through the use of GR24, a synthetic analog of this class of molecules. Strigolactone mutants show a bushy phenotype and GR24 application inhibits the growth of axillary buds in these mutants, thus restoring the phenotype of a wild plant, which is characterized by a stronger apical dominance. In this work, we tested the effectiveness of this chemical on pea (Pisum sativum) plants following apex removal, which disrupts apical dominance and leads to axillary bud outgrowth. Moreover, we searched for relationships between the response to the strigolactone and gibberellin metabolism by applying GR24 to both climbing and dwarf peas, the latters being mutants for gibberellin biosynthesis. The results suggest that the endogenous level of the bioactive gibberellin GA1 might modulate the response of decapitated pea plants to GR24, by changing bud sensitivity to the applied strigolactone

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“…Compounds structurally related to strigolactone are potent synthetic germination stimulants for many Striga and Orobanche/Phelipanche species (Zwanenburg & Wigchert 1998;Wigchert et al 1999) but their usage in the field has not been practical up to now due to the high cost involved in their synthesis and also their instability in soils with high pH (Dhanapal & Struik 1996;Kroschel 2001;Lopez-Raez et al 2008). Therefore, detailed studies are needed to solve the issues about the cost and the formulation of the substances, which are affecting the efficiency and stability of them in the field conditions.…”

Section: Resultsmentioning

confidence: 99%

“…The application of germination stimulants to induce suicidal seed germination of parasitic weeds appears attractive for biosafety reasons, rapid soil decomposition, and high biological activity at very low application rates (Elzein & Kroschel 2004). Compounds structurally related to strigolactone are potent synthetic germination stimulants for many Orobanche species (Zwanenburg & Wigchert 1998;Wigchert et al 1999). Synthetic strigolactone analogues of the strigolactones have been synthesized by several working groups: Johnson, Welzel, Zwanenburg (Wegman 2006).…”

Section: Introductionmentioning

confidence: 99%

Potency of some synthetic stimulants and root exudates on the germination of Phelipanche spp.

UYGUR¹

2013

Tarım Bilimleri Dergisi

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Broomrapes (Orobanche/Phelipanche spp.) are considered as the most important problem of some cultivated plants, especially belonging to Solanaceae and Fabaceae families. Phelipanche ramosa L. and Phelipanche aegyptiaca (L.) Pers. cause serious problems especially in tomato grown areas in Turkey and the other Mediterranean countries. This study was conducted to determine the effect of some synthetic stimulant substances and root exudates on the germination rate of the broomrape species under controlled laboratory conditions. GR24 (0.1-1 ppm), GR7 (0.1-1 ppm) and GA3 (10 ppm) were used as synthetic germination stimulant substances; otherwise flax, cotton, soybean, bean, pea, cowpea, tomato, lentil, cucumber and tobacco seedlings were used for root exudates. According to average results of the trials; synthetic stimulants and root secretions increased the germination rate of P. ramosa at the range of 50-77% and 0-16% respectively, otherwise average data for P. aegyptiaca are 62-95% and 0-63%. As a result of the studies it was determined that synthetic stimulants were significantly increased the germination rate of Phelipanche species.

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Dose−Response of Seeds of the Parasitic WeedsStrigaandOrobanchetoward the Synthetic Germination Stimulants GR 24 and Nijmegen 1

Cited by 75 publications

References 27 publications

Stable transformation and actin visualization in callus cultures of dodder (Cuscuta europaea)

Stable transformation and actin visualization in callus cultures of dodder (Cuscuta europaea)

Strigolactone may interact with gibberellin to control apical dominance in pea (Pisum sativum)

Potency of some synthetic stimulants and root exudates on the germination of Phelipanche spp.

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