Dose Response of Milk Fat to Intravenous Administration of the trans-10, cis-12 Isomer of Conjugated Linoleic Acid

Viswanadha, Srikant; Giesy, J.G.; Hanson, Travis W.; McGuire, Mark A.

doi:10.3168/jds.s0022-0302(03)73926-5

Cited by 18 publications

(22 citation statements)

References 31 publications

(50 reference statements)

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“…This differs from other studies conducted with cows during established lactation, in which feeding rumen-protected CLA [6-9] or abomasal infusion of CLA [10-13] or intravenous infusions of CLA [14] resulted in a reduction of milk fat content. An explanation for the lack of a CLA response in milk fat during the first few weeks postpartum is unknown [8].…”

Section: Discussioncontrasting

confidence: 71%

Rumen-protected conjugated linoleic acid supplementation to dairy cows in late pregnancy and early lactation: effects on milk composition, milk yield, blood metabolites and gene expression in liver

Sigl

Schlamberger

Kienberger³

et al. 2010

Acta Vet Scand

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BackgroundConjugated linoleic acid (CLA) is a collective term for isomers of octadecadienoic acid with conjugated double-bond system. Thus, it was the objective to investigate whether milk composition and metabolic key parameters are affected by adding CLA to the diet of dairy cows in the first four weeks of lactation.MethodsA study was carried out with five primiparous cows fed a CLA supplemented diet compared to five primiparous cows without CLA supplementation. CLA supplemented cows received 7.5 g CLA/day (i.e. 50% cis(c)9,trans(t)11- and 50% t10,c12-CLA) starting two weeks before expected calving and 20 g CLA/day (i.e. 50% c9,t11- and 50% t10,c12-CLA) throughout day 1 to 28 of lactation.ResultsThe CLA supplement was insufficiently accepted by the animals: only 61.5% of the intended amount was ingested. Fed CLA were detectable in milk fat, whereas contents of c9,t11-CLA and t10,c12-CLA in milk fat were higher for CLA supplemented cows compared to the control group. On average over the entire treatment period, there was a decrease of saturated fatty acids (FA) in milk fat of CLA supplemented cows, combined with a higher content of monounsaturated and trans FA.Our study revealed no significant effects of c9,t11- and t10,c12-CLA supplementation either on milk yield and composition or on metabolic key parameters in blood. Furthermore the experiment did not indicate significant effects of c9,t11- and t10,c12-CLA-supplementation on gene expression of peroxisome proliferator-activated receptor-alpha (PPARα), PPARγ, sterol regulatory element-binding protein-1 and tumor necrosis factor-alpha in liver tissue.ConclusionsFeeding c9,t11- and t10,c12-CLA during the first weeks after calving did not affect metabolic key parameters of blood serum or milk composition of fresh cows. Milk fatty acid composition was changed by feeding c9,t11- and t10,c12-CLA resulting in higher contents of these isomers in milk fat. High contents of long chain FA in milk fat indicate that CLA supplementation during the first four weeks of lactation did not affect massive peripheral lipomobilization.

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Section: Discussioncontrasting

confidence: 71%

Rumen-protected conjugated linoleic acid supplementation to dairy cows in late pregnancy and early lactation: effects on milk composition, milk yield, blood metabolites and gene expression in liver

Sigl

Schlamberger

Kienberger³

et al. 2010

Acta Vet Scand

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“…Trans-vaccenic acid (Matreya LLC, Pleasant Gap, PA) was dissolved in 0.9% saline containing 10% intralipid (Fresenius Kabi, Seoul, Republic of Korea) to yield a 30 mg/mL concentration. After a 7-d adaptation period, one group was treated with 5 mL of TVA solution and the other group was given 5 mL of saline (10% intralipid in 0.9% saline) via a jugular vein injection as previously described (Viswanadha et al, 2003;Jin et al, 2010). Goats were given the intravenous injections twice daily 30 min after each milking.…”

Section: Methodsmentioning

confidence: 99%

Conjugated linoleic acid synthesis-related protein proteasome subunit α 5 (PSMA5) is increased by vaccenic acid treatment in goat mammary tissue

Jin

Zhang

et al. 2012

Journal of Dairy Science

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This study was conducted to identify proteins associated with the endogenous synthesis of conjugated linoleic acid (CLA) from trans-vaccenic acid (TVA; trans-11 C18:1, a precursor for CLA endogenous synthesis) in mammary tissues. Six lactating goats were divided into 2 groups. One group was given an intravenous bolus injection of TVA (150mg) twice daily over 4 d; the other group received saline injections. Treatment with TVA increased the concentration of cis-9,trans-11 CLA and TVA in goat milk. Additionally, TVA treatment increased the expression of stearoyl-CoA desaturase (SCD) in mammary tissue. Using 2-dimensional gel electrophoresis and electrospray ionization quadrupole time-of-flight mass spectrometry, 3 proteins affected by infusions of TVA were identified. Proteasome (prosome, macropain) subunit α type 5 (PSMA5) was upregulated, whereas peroxiredoxin-1 and translationally controlled tumor protein 1 were downregulated in TVA-treated animals compared with the vehicle-injected controls. Only the effect of TVA on PSMA5 could be confirmed by Western blot analysis. To further explore the regulation of PSMA5 in mammary epithelial cells when TVA is converted into CLA, we used a differentiated bovine mammary epithelial cell line treated with TVA for 6h. Changes in cis-9,trans-11 CLA concentrations and mRNA expression patterns of both SCD and PSMA5 were monitored. The concentration of cis-9,trans-11 CLA increased after TVA treatment. The mRNA expression level of PSMA5 was significantly elevated to 6h, but SCD mRNA expression only increased in 2h after TVA treatment. These results indicate that PSMA5 is highly expressed in goat mammary tissue and bovine mammary epithelial cells when TVA is converted into CLA. Our data suggest that PSMA5 protein is associated with CLA biosynthesis in mammary tissue.

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“…In many studies, the addition of CLA has had a specific inhibitory effect on milk fat content (Bauman and Griinari, 2003). Abomasal (Chouinard et al, 1999;Baumgard et al, 2001) and intravenous (Viswanadha et al, 2003) infusion and dietary CLA supplementation (Giesy et al, 2002) reduce milk fat without affecting milk lactose, protein, or yield. A similar situation exists in diet-induced milk fat depression (MFD), a naturally occurring condition in which the reduction of milk fat is associated with the alteration of milk fatty acid profile (Bauman et al, 2008), supporting experimental observations.…”

Section: Introductionmentioning

confidence: 99%

Conjugated linoleic acid-induced milk fat reduction associated with depressed expression of lipogenic genes in lactating Holstein mammary glands

Han¹,

Pang²,

Li³

et al. 2012

Genet. Mol. Res.

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ABSTRACT. The efficacy of conjugated linoleic acid (CLA) in diet supplements for milk fat reduction is well documented in several species. However, the mechanisms by which fatty acids regulate mammary lipogenesis remain largely unknown, especially with regard to gene expression of enzyme and regulators. In this study, 8 Holstein dairy cows in their mid-lactation period were randomly divided into 2 groups. Control cows received a Ca salt of palm oil fatty acid dietary supplement, and those in the CLA group were fed Ca salts of CLA (Ca-CLA), all in a dose of approximately 200 g•cow -1•day -1 for 14 days. The milk yield was recorded daily, and protein, lactose, and fat in the milk were quantified every 3 4755 ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 11 (4): 4754-4764 (2012) CLA reduces lipogenic gene expression days for 2 weeks. Fatty acids in the milk were analyzed with gas-liquid chromatography. Measurement of messenger RNA levels of the main lipogenic genes of lipoprotein lipase, acetyl-coenzyme A (CoA) carboxylase, fatty acid synthase, stearoyl-CoA desaturase, and transcription factors such as sterol response element binding protein 1 (SREBP1) and peroxisome proliferator-activated receptor γ was performed in biopsy samples of mammary tissue on the last day. The results indicated that dietary Ca-CLA caused a continuous reduction of milk fat (P < 0.01) with no effect on milk yield, milk protein, and lactose. The fatty acid profile in the milk from the CLA group differed from that from controls, and the yield of milk fatty acid decreased (P < 0.01) with Ca-CLA supplementation. The depressed expression of lipogenic genes (lipoprotein lipase, acetyl-CoA carboxylase, fatty acid synthase, and stearoyl-CoA desaturase) demonstrated inhibition of fatty acid de novo synthesis and uptake in the mammary gland of the CLA group. Furthermore, the gene expression of transcription factor SREBP1 was also downregulated (P < 0.01), but peroxisome proliferator-activated receptor γ was unchanged, suggesting that SREBP1 may play a key role in the regulation of lipogenic gene expression in the lactating mammary gland.

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Dose Response of Milk Fat to Intravenous Administration of the trans-10, cis-12 Isomer of Conjugated Linoleic Acid

Cited by 18 publications

References 31 publications

Rumen-protected conjugated linoleic acid supplementation to dairy cows in late pregnancy and early lactation: effects on milk composition, milk yield, blood metabolites and gene expression in liver

Rumen-protected conjugated linoleic acid supplementation to dairy cows in late pregnancy and early lactation: effects on milk composition, milk yield, blood metabolites and gene expression in liver

Conjugated linoleic acid synthesis-related protein proteasome subunit α 5 (PSMA5) is increased by vaccenic acid treatment in goat mammary tissue

Conjugated linoleic acid-induced milk fat reduction associated with depressed expression of lipogenic genes in lactating Holstein mammary glands

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