Dose–Response Effects of 3-Nitrooxypropanol Combined with Low- and High-Concentrate Feed Proportions in the Dairy Cow Ration on Fermentation Parameters in a Rumen Simulation Technique

Schilde, Matthias; Soosten, Dirk von; Hüther, Liane; Kersten, Susanne; Meyer, Ulrich; Zeyner, Annette; Dänicke, Sven

doi:10.3390/ani11061784

Cited by 11 publications

(5 citation statements)

References 41 publications

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“…Roque et al (2018) used the polypropylene RUSITEC vessels to test the effects of 5% DM Asparagopsis taxiformis on in vitro rumen fermentation and subsequent in vivo experiments showed similar rumen responses [23,24]. More recently, the effect of 3-Nitrooxypropanol (3-NOP) was explored in relation to changing concentrate ratios of feed rations for dairy cattle and the RUSITEC results [25] were supported in vivo [26]. Whereas these in vitro/in vivo study pairs support our hypothesis that RUSITEC systems can be used to adequately mimic in vivo rumen fermentation and its response to feed additives, the Ankom system has also been used successfully to predict changes in enteric methane production [13].…”

Section: Its Sequencing Resultsmentioning

confidence: 99%

Comparison of three artificial rumen systems for rumen microbiome modeling

Shaw

Park

González

et al. 2022

Preprint

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Background The rumen contains a complex mixture of microbes, which are crucial for ruminant health and feed fermentation. During the fermentation process some of the feed-derived carbon becomes carbon dioxide and methane, which are released into the atmosphere where they act as greenhouse gases and contribute to climate change. There is growing interest in reducing the loss of feed-derived carbon and making it available to the animal, improving animal productivity, while also reducing the carbon footprint of the ruminant industry. To this end, artificial rumen systems (ARS) have been used for evaluating novel feed additives for their effect on the rumen microbiome and rumen function prior to conducting resource intensive animal trials. Whereas ARS are capable of predicting the response of the rumen and its microbiome, it is unclear how accurately different in vitro systems simulate the natural system and how results compare between the artificial systems that are being employed. Here we evaluated physical, chemical and microbiome metrics of three ARS over five days and compared them to those metrics in the in vivo rumen. Results Over a 48 hrs sampling period, the batch style platform (Ankom) was able to replicate pH, volatile fatty acid profile, and bacterial and fungal microbiome of the in vivo rumen, but its accuracy of mimicking in vivo metrics dropped significantly beyond 48 hrs. In contrast, the semi-continuous RUSITEC models, RUSITEC PP and RUSITEC prime, were able to mimic the volatile fatty acid profile and microbiota of the in vivo rumen for up to 120 hrs of rumen simulation. Comparison of gas production across vessel types demonstrated that the semi-continuous RUSITEC platforms display less variability among vessel replicates and time compared to the Ankom system. Conclusions In this study, we found that three widely used ARS were able to simulate the rumen ecosystem adequately for the first 48 hrs, with predictions from the more advanced semi-continuous ARS being more accurate when simulations extended over 48 hrs. Findings of this study will help to select the appropriate in vitro system for evaluating the response of the complex rumen microbiome to feed additives. Further work is necessary to improve the capabilities of these platforms and to standardize the methodology for large-scale application.

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Section: Its Sequencing Resultsmentioning

confidence: 99%

Comparison of three artificial rumen systems for rumen microbiome modeling

Shaw

Park

González

et al. 2022

Preprint

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“…El 3-nitrooxipropanol es un compuesto relativamente nuevo que ha tenido buena aceptación en materia de mitigación de metano ruminal (Jayanegara et al 2018, Zhang et al 2018. Se ha reportado que este compuesto a dosis de 200 y 160 mg/kg (materia seca) inhibió en 78 y 40 % la producción de metano ruminal de dietas altas en grano usando el sistema RUSITEC (Romero-Pérez et al 2017, Schilde et al 2021. Esto sugiere que la LOV puede mantener un nivel cercano a inhibidores de la metanogénesis introducidos más recientemente, como es el caso del 3-nitrooxipropanol.…”

Section: Resultados Y Discusiónunclassified

Inhibición in vitro de la metanogénesis ruminal de una dieta alta en grano con lovastatina

Ábrego-García

Poggi‐Varaldo²,

Robles-González³

et al. 2022

Rev. Int. Contam. Ambie.

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Uno de los mayores impactos ambientales de la ganadería es la emisión de metano entérico, un potente gas de efecto invernadero. En este contexto, se han evaluado algunos compuestos antimetanogénicos tales como la lovastatina (LOV), un inhibidor de la hidroximetilglutaril-CoA reductasa, enzima clave para la síntesis de membrana en arqueas metanogénicas. Esta podría ser una opción para mitigar las emisiones de metano ruminal. Sin embargo, los experimentos in vitro son escasos y no existe un criterio general sobre la mitigación de metano con LOV en estas condiciones. Por lo tanto, el objetivo de esta investigación fue evaluar el efecto in vitro de la LOV sobre la metanogénesis y características de la fermentación ruminal, así como comparar los resultados con aquellos de la monensina (MON) y el ácido bromoetano sulfónico (BES). Se realizó una prueba in vitro en lote. Se utilizó una ración alta en grano, fluido ruminal de bovinos y tres inhibidores metanogénicos. El diseño experimental fue completamente al azar con tres tratamientos (LOV, MON, BES) y un testigo sin inhibidor. En este trabajo la LOV mostró efectos alentadores sobre la mitigación de la metanogénesis en la fermentación ruminal (43.56 %) sin afectar la producción total y perfil de AGV. Sin embargo, la dosis para obtener este resultado fue mayor en comparación con las de los otros tratamientos. La MON fue la más efectiva con relación a la concentración/inhibición de la metanogénesis, seguida del BES. Ambos tratamientos presentaron cambios en el perfil de AGV.

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“…For example, Roque et al (2018) used the RUSITEC PP system to test the effects of 5% DM Asparagopsis taxiformis on in vitro rumen fermentation and subsequently showed a similar rumen response in vivo [38,39]. More recently, the effect of 3-Nitrooxypropanol (3-NOP) was explored in relation to changing concentrate ratios of feed rations for dairy cattle, and the RUSITEC results [40] were supported in vivo [41]. Whereas these in vitro/in vivo study pairs support our hypothesis that RUSITEC systems can be used to adequately mimic in vivo rumen fermentation and its response to feed additives in long-term studies, the Ankom system has also been used successfully to predict changes in the enteric methane production [11].…”

Section: Discussionmentioning

confidence: 99%

A Comparison of Three Artificial Rumen Systems for Rumen Microbiome Modeling

Shaw,

Park,

Gonzalez

et al. 2023

Fermentation

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The rumen contains a complex mixture of microbes, crucial for the animal’s ability to degrade feed. Some of the feed-derived carbon is released as methane, a potent greenhouse gas, into the atmosphere. There is growing interest in reducing the loss of feed-derived carbon, making it available to the animal and improving animal productivity. Artificial rumen systems (ARSs) have been widely used to evaluate novel feed additives in terms of their ability to reduce methane production in the rumen and their effect on the rumen microbiome function prior to conducting resource-intensive animal trials. While the value of ARSs is widely acknowledged, it remains unclear which of these in vitro systems simulate the natural system most accurately. Here, we evaluated three different ARSs and compared them to in vivo rumen metrics. The results showed that all systems were capable of maintaining stable pH, redox potential, and temperature over time. The batch-style ARS simulated the rumen over 48 h. The semi-continuous ARS mimicked the volatile fatty acid profile and microbiota of the in vivo rumen for up to 120 h. Similarly, all ARSs maintained the prokaryotic and eukaryotic rumen populations over the duration of the study, with the semi-continuous ARS maintaining the natural rumen microbiome more accurately and for up to 120 h. In sum, our results suggest that three of the widely used ARSs simulate the rumen ecosystem adequately for many short-term rumen microbiome studies, with the more advanced semi-continuous ARS being more accurate when rumen simulation is extended to over 48 h.

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Dose–Response Effects of 3-Nitrooxypropanol Combined with Low- and High-Concentrate Feed Proportions in the Dairy Cow Ration on Fermentation Parameters in a Rumen Simulation Technique

Cited by 11 publications

References 41 publications

Comparison of three artificial rumen systems for rumen microbiome modeling

Comparison of three artificial rumen systems for rumen microbiome modeling

Inhibición in vitro de la metanogénesis ruminal de una dieta alta en grano con lovastatina

A Comparison of Three Artificial Rumen Systems for Rumen Microbiome Modeling

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