Dose-Response Effect of Dietary Docosahexaenoic Acid on Fatty Acid Profiles of Serum and Tissue Lipids in Rats

Abstract

The dose-response effects of dietary docosahexaenoic acid (22:6n-3, DHA) on the fatty acid profiles of total lipids of rat serum and tissues were investigated. Rats were fed diets containing graded levels of purified DHA at 0, 1.0, 3.4, and 8.7% of total energy in the diets for 2 weeks. It was found that each tissue had its own peculiar composition of fatty acids which differed markedly from that of circulating serum lipid. This composition was basically influenced dose-dependently by the dietary lipids with graded levels of DHA, but to different degrees in different tissues. Those of brain were most resistant and of heart most susceptible to dietary DHA.

Docosahexaenoic Acid-Induced Lipid Peroxidation and Urinary Excretion of Mercapturic Acid in Rats

“…One of the reasons why the PCOOH and PEOOH levels did not increase in the liver, even after the intake of highly unsaturated DHA, seems to be very rapid progress of lipid peroxidation from the initial stage, when these hydroperoxides are generated, to propagation stage producing TBA-reactive substances, because the proportions of highly unsaturated PUFAs such as EPA, 22:5n-3, and DHA become higher in the liver, and conversely those of LA and arachidonic acid, which have a low degree of unsaturation, become lower after DHA intake, as observed previously [7,10,33]. However, free MDA + 4-HAE did not increase even in the propagation stage of lipid peroxidation in the liver.…”

“…Considering the PUFA composition of the kidney [7,10,33], the proportions of n-6 PUFAs with a low degree of unsaturation are generally higher, and that of highly unsaturated DHA is lower, than in the liver. In addition, high retroconversion from DHA to EPA was shown in the kidney [7,10,33]. Furthermore, although the Vit.…”

Induction of Multidrug Resistance-Associated Protein MRP3 in the Liver of Rats Fed with Docosahexaenoic Acid

“…9,18,38) The TBA values in the tissues were also significantly higher in the DHA group than in the LA group (Fig. 1A).…”

Role of phospholipase A2s and lipid mediators in secondary damage after spinal cord injury

“…Therefore, diet can induce changes in PUFA levels that alter PUFA composition in the CNS (Chernenko et al 1989;Saito et al 1998). A continuous intake of DHA is likely to increase its content in spinal cord membrane phospholipids, and subsequently, increase its availability after injury (Rapoport et al 2001).…”