Dose response chemopreventive potential of allyl isothiocyanate against 7,12-dimethylbenz(a)anthracene induced mammary carcinogenesis in female Sprague-Dawley rats

Rajakumar, Thangarasu; Pugalendhi, Pachaiappan; Thilagavathi, Subbaiyan

doi:10.1016/j.cbi.2015.02.015

Cited by 39 publications

(20 citation statements)

References 61 publications

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“…ROS augments the peroxidation of membrane lipids and contributes to the initiation and progression of cancer thus affecting the normal biochemical processes which results in body weight loss (Saha et al, ). In this study, loss in body weight and tissue weight followed by increased tumor volume in rats treated with DMBA clearly indicate that the energy metabolism got changed during tumor formation (Rajakumar, Pugalendhi, & Thilagavathi, ; Shimeda et al, ). A previous report stated that the decreased body weight among STZ‐induced diabetic rats got reversed when citronellol was administered that is, the body weight got increased which may be due to antilipidemic property (Srinivasan & Muruganathan, ).…”

Section: Discussionmentioning

confidence: 50%

RETRACTED: Dose‐dependent chemopreventive effects of citronellol in DMBA‐induced breast cancer among rats

Jayaganesh

Pugalendhi

Subramaniyan

2019

Drug Development Research

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Breast cancer is one of the most common cancers among women world wide and its incidence is on tremendous increase. The present study is aimed to analyze the dose‐dependent chemopreventive efficacy of citronellol on 7,12‐dimethylbenz(a)anthracene (DMBA)‐induced rat mammary carcinogenesis. The mammary tumor was induced through a single dose of DMBA (25 mg/rat) injected subcutaneously near the mammary gland of rats. In DMBA‐injected rats, 100% tumor incidence, increased tumor volume, and tumor burden along with loss of body weight were observed. Biochemical analysis revealed the increased levels of phase I detoxification proteins (cytochrome P450 and b5) and decreased activities of phase II detoxification enzymes (glutathione‐S‐transferase and glutathione reductase) in hepatic and mammary tissues. The levels of enzymatic and non‐enzymatic antioxidants (superoxidedismutase, catalase, glutathione peroxidase, and (GPx) and reduced glutathione) were decreased and lipid peroxidation by‐products (thiobarbituric acid reactive substance and lipid hydroperoxide) got increased in plasma and mammary tissues. Oral administration of different doses of citronellol (25, 50, and 100 mg/kg body weight) to DMBA‐treated rats for 16 weeks absolutely inhibited the tumor incidence and restored the biochemical parameters near to normal level in 50 and 100 mg doses whereas the histopathological studies also supported the biochemical findings. Hence, the result suggests that the citronellol of 50 mg/kg body weight exerted significant chemopreventive effects and can be considered as a minimum optimum dose in the prevention of mammary carcinogenesis.

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Section: Discussionmentioning

confidence: 50%

RETRACTED: Dose‐dependent chemopreventive effects of citronellol in DMBA‐induced breast cancer among rats

Jayaganesh

Pugalendhi

Subramaniyan

2019

Drug Development Research

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“…Human absorption and disposition of allyl isothiocyanate appear to closely resemble that of animals, as studies showed that about 50% of the dose was recovered in the urine as a metabolite [78, 79]. Allyl isothiocyanate inhibited mammary tumor incidence and tumor growth in DMBA-treated female Sprague Dawley (SD) rats [80]. In this study, allyl isothiocyanate decreased lipid peroxidation-related thiobarbituric acid reactive substances (TBARS) and lipid hydroperoxides (LOOH) as well as total cholesterol (TC), triglycerides (TG), glycophospholipid (PL), free fatty acids (FFA), low-density lipoprotein (LDL) and very-low density lipoprotein (VLDL) [80].…”

Section: Natural Products Against Er-positive Breast Cancermentioning

confidence: 99%

“…Allyl isothiocyanate inhibited mammary tumor incidence and tumor growth in DMBA-treated female Sprague Dawley (SD) rats [80]. In this study, allyl isothiocyanate decreased lipid peroxidation-related thiobarbituric acid reactive substances (TBARS) and lipid hydroperoxides (LOOH) as well as total cholesterol (TC), triglycerides (TG), glycophospholipid (PL), free fatty acids (FFA), low-density lipoprotein (LDL) and very-low density lipoprotein (VLDL) [80]. Decreased levels of CYP450 and increased activities of phase II and antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), GST and glutathione reductase (GR), have been shown in liver and mammary tissues of allyl isothiocyanate-treated rats [80].…”

Section: Natural Products Against Er-positive Breast Cancermentioning

confidence: 99%

Role of dietary bioactive natural products in estrogen receptor-positive breast cancer

Bak

Gupta

Wahler

et al. 2016

Seminars in Cancer Biology

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Estrogen receptor (ER)-positive breast cancer, including luminal-A and -B, is the most common type of breast cancer. Extended exposure to estrogen is associated with an increased risk of breast cancer. Both ER-dependent and ER-independent mechanisms have been implicated in estrogen-mediated carcinogenesis. The ER-dependent pathway involves cell growth and proliferation triggered by the binding of estrogen to the ER. The ER-independent mechanisms depend on the metabolism of estrogen to generate genotoxic metabolites, free radicals and reactive oxygen species to induce breast cancer. A better understanding of the mechanisms that drive ER-positive breast cancer will help optimize targeted approaches to prevent or treat breast cancer. A growing emphasis is being placed on alternative medicine and dietary approaches toward the prevention and treatment of breast cancer. Many natural products and bioactive compounds found in foods have been shown to inhibit breast carcinogenesis via inhibition of estrogen induced oxidative stress as well as ER signaling. This review summarizes the role of bioactive natural products that are involved in the prevention and treatment of estrogen-related and ER-positive breast cancer.

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“…7,12‐Dimethylbenz(γ)anthracene (DMBA), a polyphenolic aromatic hydrocarbon is commonly used as a chemical carcinogen to induce mammary cancer in experimental animals (Rajkumar et al, ; Ray et al, ). After metabolism, DMBA turns into epoxide that readily forms DNA adducts responsible for the malignant transformations (Rajkumar et al, ).…”

Section: Introductionmentioning

confidence: 99%

Potentiating apoptosis and modulation of p53, Bcl2, and Bax by a novel chrysin ruthenium complex for effective chemotherapeutic efficacy against breast cancer

Roy¹,

Sil²,

Chakraborty³

2018

Journal Cellular Physiology

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Breast cancer is the most frequent cause of cancer in women. In the current study, transition metal ruthenium was complexed with flavonoid chrysin to evaluate the chemotherapeutic potential of this compound in Michigan Cancer Foundation-7 (MCF-7) human mammary cancer cell line and 7,12-dimethylbenz(α)anthraceneinduced mammary cancer in female Sprague-Dawley rats. The characterizations of the complex were accomplished through UV-visible, NMR, IR, Mass spectra, and XRD techniques and antioxidant activity was assessed by DPPH, FRAP, and ABTS methods.In vitro studies included cell viability, cell cycle analysis, DNA fragmentation, and marker analysis by western blot analysis and found that complex treatment suppressed cell growth-induced cell cycle arrest and enhanced the induction of apoptosis in cancer cells. Moreover, complex treatment modulated signaling pathways including mTOR, VEGF, and p53 in the MCF-7 cells. Acute and subacute toxicity was performed in rats to determine the therapeutic doses. Breast cancer in rats was initiated by the administration of 7,12-dimethylbenz(α)anthracene (0.5 mg/100 g body weight) via single tail vein injection. The histopathological analysis after 24 weeks of carcinogenesis study depicted substantial repair of hyperplastic lesions. Immunohistochemical analysis revealed upregulation of Bax and p53 and downregulation of Bcl2 proteins and TUNEL assay showed an increase in apoptotic index in ruthenium-chrysin-treated groups as compared to the carcinogen control. Our findings from the in vitro and in vivo study support the continued investigation of ruthenium-chrysin complex possesses a potential chemotherapeutic activity against breast cancer and was efficient in reducing hyperplastic lesions in the mammary tissues of rats by inducing apoptosis.

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Dose response chemopreventive potential of allyl isothiocyanate against 7,12-dimethylbenz(a)anthracene induced mammary carcinogenesis in female Sprague-Dawley rats

Cited by 39 publications

References 61 publications

RETRACTED: Dose‐dependent chemopreventive effects of citronellol in DMBA‐induced breast cancer among rats

RETRACTED: Dose‐dependent chemopreventive effects of citronellol in DMBA‐induced breast cancer among rats

Role of dietary bioactive natural products in estrogen receptor-positive breast cancer

Potentiating apoptosis and modulation of p53, Bcl2, and Bax by a novel chrysin ruthenium complex for effective chemotherapeutic efficacy against breast cancer

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