Dose rates of antimicrobial substances in boar semen preservation—time to establish new protocols

Schulze, Martin; Grobbel, Mirjam; Riesenbeck, A; Brüning, Sabine; Schaefer, Joel J.; Jung, Markus; Grossfeld, R.

doi:10.1111/rda.12921

Cited by 22 publications

(22 citation statements)

References 17 publications

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“…This study was pioneering at comparing gentamicin with several other antimicrobials such as ampicillin, apramycin and cefoxitin. Currently, gentamicin has established itself as one of the most used antimicrobials in boar semen diluents (Schulze et al, 2017). However, Gączarzewicz et al (2016) demonstrated that the inhibitory activity of even gentamicin may be limited during long-term preservation (16°C for five days) in diluent (X-cell ® ).…”

Section: Use Of Antibacterial Substances In Semen Extendersmentioning

confidence: 99%

Current and alternative trends in antibacterial agents used in mammalian semen technology

Santos¹,

Silva²

2020

Anim Reprod

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How to cite: Santos CS, Silva AR. Current and alternative trends in antibacterial agents used in mammalian semen technology. AbstractThe use of antibacterial substances as additives in extenders for ensuring the sanitary quality of the semen employed in reproductive biotechniques and preserving it from bacterial deterioration has been reported since the mid-twentieth century. However, the deleterious effects of these drugs on the sperm quality as well as their effectiveness in controlling bacterial growth in the preserved semen have been questioned. The aim of this review was to report the antimicrobials primarily used in the extenders added to the semen of mammals, and to present alternatives to their use. Among the various mammalian species, there is a large variation regarding the antimicrobial types added to semen extenders as cephalosporins (ceftiofur, cefdinir, eg) and quinolones (ofloxacin, ciprofloxacin), alone or in combination with large action spectra substances as penicillin-streptomycin and gentamicin-tylosin-lincomycin-spectinomycin. To combat problems related to bacterial resistance to these drugs, the emergence of alternatives is increasingly evident. Among these alternatives, use of physical methods as centrifugation and filtration, as well as the use of antimicrobial peptides and other substances from different origins have been highlighted for presenting antimicrobial potential.

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Section: Use Of Antibacterial Substances In Semen Extendersmentioning

confidence: 99%

Current and alternative trends in antibacterial agents used in mammalian semen technology

Santos¹,

Silva²

2020

Anim Reprod

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“…One subset was validated individually and the remaining 4 subsets for training in Google's TensorFlow [28]. Three method, dropout [29], data augmentation [30] and early stopping [31], were used to minimize the overfitting risk. Technical details were described in supplementary methods and 7…”

Section: Training Of Cnn Modelsmentioning

confidence: 99%

A deep learning-based system for identifying differentiation status and delineating the margins of early gastric cancer in magnifying narrow-band imaging endoscopy

Ling

et al. 2020

Endoscopy

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INTRODUCTION:Accurate identification of early gastric cancer (EGC) differentiation status and margins is critical for determining the surgical strategy and achieving curative resection in EGC patients. The aim of this study was to develop a real-time system for accurately identifying differentiation status and delineating margins of EGC in Magnifying Narrow-band Imaging (ME-NBI) endoscopy. METHODS:2217 images from 145 EGC patients and 1870 images from 139 EGC patients were retrospectively collected to train and test the convolutional neural network (CNN) 1 for identifying EGC differentiation status. 882 images from 58 EGC patients were used to compare the performance of CNN1 with that of experts. 928 images from 132 EGC patients and 742 images from 87 EGC patients were used to train and test CNN2 for delineating EGC margins. RESULTS:The system correctly predicted differentiation status of EGCs with an accuracy of 83.3% (95%CI: [81.5%-84.9%]) in testing dataset. In the man-machine contest, CNN1 performed significantly better compared to the five experts [86.2% (95%CI: [75.1%-92.8%]) vs. 69.66 (95%CI: [64.1%-74.7%])). For delineating EGC margins, the system achieved an accuracy of 82.7% (95%CI: [78.6%-86.1%]) in differentiated EGC and 88.1% (95%CI: [84.2%-91.1%]) in undifferentiated EGC under an overlap ratio of 0.80. In unprocessed EGD videos, the system achieved real-time EGC differentiation status diagnosis and EGC margin delineation in ME-NBI endoscopy. CONCLUSION:We developed a deep learning-based system for accurately identifying differentiation status and delineating margins of EGC in ME-NBI endoscopy. This system achieved a superior performance when compared with experts, and was successfully tested in real EGC videos.

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“…Numerous studies have indicated that the addition of antibacterials to semen extenders is an effective method to inhibit bacterial growth, prolong sperm preservation time in vitro, and increase conception rates (Bresciani et al, ; Yaniz, Marco‐Aguado, Mateos, & Santolaria, ). Common antibiotics included penicillin, streptomycin, and gentamicin (Oplinger & Wagner, ; Schulze et al, ). However, the long‐term use of antibiotics has led to an increase in the drug resistance of certain bacteria and the therapeutic effects of the medicines have been significantly reduced (Speck et al, ).…”

Section: Introductionmentioning

confidence: 99%

Effects of sulfanilamide on boar sperm quality, bacterial composition, and fertility during liquid storage at 17°C

Feng

Ren

Qian

et al. 2019

Animal Science Journal

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Sulfanilamide (SA) is an effective broad‐spectrum antibacterial agent in human and veterinary medicine. The purpose of this study was to evaluate the effects of SA on boar sperm quality during liquid storage at 17°C and determine the optimal concentration of SA and its effects on bacterial growth, microbial composition, and maternal fertility. Boar ejaculates were diluted with a basic extender, containing different concentrations of SA, and stored in a 17°C incubator for 6 days. The sperm motility, plasma membrane integrity, and acrosome integrity were measured daily. The results showed that when the concentration of SA was 0.02 g/L, the sperm quality parameters were significantly higher than those of all other treatment groups (p < .05). We also monitored the bacterial growth and compared the differences in the microbial species between the 0.02 g/L SA group and the control by 16S rDNA sequencing. The results revealed that some bacteria, such as Staphylococcus and Pseudomonas, were considerably lower in the 0.02 g/L SA group than in the control group (p < .05). In addition, preserved semen was used for artificial insemination, and results showed that 0.02 g/L SA group had a higher litter size, and its pregnancy rate was 92.5%.

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Dose rates of antimicrobial substances in boar semen preservation—time to establish new protocols

Cited by 22 publications

References 17 publications

Current and alternative trends in antibacterial agents used in mammalian semen technology

Current and alternative trends in antibacterial agents used in mammalian semen technology

A deep learning-based system for identifying differentiation status and delineating the margins of early gastric cancer in magnifying narrow-band imaging endoscopy

Effects of sulfanilamide on boar sperm quality, bacterial composition, and fertility during liquid storage at 17°C

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