Dose estimation by chromosome aberration analysis and micronucleus assays in victims accidentally exposed to<sup>60</sup>Co radiation

Liu, Q; Cao, Jia; Wang, Z Q; Bai, Yongli; Lu, Yehu; Huang, Qian; Zhao, Wei; Li, J; Jiang, Li; Tang, W.-S.; Fu, Bao Hua; Fan, Feiyue

doi:10.1259/bjr/62484075

Cited by 27 publications

(14 citation statements)

References 13 publications

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“…The chromosomes of peripheral blood lymphocytes may be damaged by small doses of radiation leading to chromosomal aberrations. Thus, the estimation of a radiation dose by analyzing chromosome aberrations in peripheral blood lymphocytes has been widely recognized as a diagnostic method (15)(16)(17). In the present study, the rate of dicentric and centric-ring aberrations was 4.82±0.65% at 1 month after exposure, and was decreased by 80% at 1 year after exposure.…”

Section: Discussionsupporting

confidence: 48%

Long-term health effects of persistent exposure to low-dose lr192 gamma-rays

Wang

Jiang

et al. 2016

Experimental and Therapeutic Medicine

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The aim of the present study was to investigate the effect of persistent low-dose iridium-192 (Ir192) exposure on immunological function, chromosome aberration and the telomerase activity of bone marrow mononuclear cells (BMNCs), in order to increase clinical knowledge of the late effects of persistent low-dose Ir192 gamma-ray exposure. Patients (n=54) accidentally exposed to persistent low-dose Ir192 were included in this 10-year follow-up study. Clinical symptoms, peripheral blood, bone marrow, cellular and humoral immune status, chromosome aberrations and the telomerase activity of BMNCs were analyzed in this study. Exposure to low-dose Ir192 resulted in different degrees of clinical symptoms and significantly lowered complement C3 and C4 levels, CD3, CD4 and CD8 T cell levels, the lymphocyte transformation rate and the percentage of natural killer (NK) cells. It also led to increases in peripheral blood and bone marrow abnormality rates, chromosome aberration rate and BMNC telomerase activity. Exposure to persistent low-dose Ir192 radiation resulted in different degrees of immune dysfunction, and abnormalities of blood cells and bone marrow, which recovered within 1-3 years. Chromosome aberrations were observed to take 5-10 years to recover. However, it would take >10 years for the telomerase activity of BMNCs to be reduced to normal levels. A prolonged follow-up time is required in order to monitor clonal proliferative diseases such as leukemia.

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Section: Discussionsupporting

confidence: 48%

Long-term health effects of persistent exposure to low-dose lr192 gamma-rays

Wang

Jiang

et al. 2016

Experimental and Therapeutic Medicine

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“…Cytogenetic analysis has been used for many decades as a tool to determine mutagenic and carcinogenic risks in both domestic and occupational settings and the detection of chromosomal aberrations (CAs) has been associated with numerous chemicals [132–134] and various types of radiation, including both low and high-LET exposures [135–138], in addition to data from atomic bomb survivors [139,140], radiotherapy patients [141–143] and even underground water-well workers [144]. Attempts have also been made to estimate prior exposure through CA analysis [145,146]. The focus here will be placed on reviewing the effects of radon exposure, along with its surrogates, on CAs (Table 4).…”

Section: Chromosome Aberrationsmentioning

confidence: 99%

The Cellular and Molecular Carcinogenic Effects of Radon Exposure: A Review

Robertson

Allen

Laney

et al. 2013

IJMS

120

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Radon-222 is a naturally occurring radioactive gas that is responsible for approximately half of the human annual background radiation exposure globally. Chronic exposure to radon and its decay products is estimated to be the second leading cause of lung cancer behind smoking, and links to other forms of neoplasms have been postulated. Ionizing radiation emitted during the radioactive decay of radon and its progeny can induce a variety of cytogenetic effects that can be biologically damaging and result in an increased risk of carcinogenesis. Suggested effects produced as a result of alpha particle exposure from radon include mutations, chromosome aberrations, generation of reactive oxygen species, modification of the cell cycle, up or down regulation of cytokines and the increased production of proteins associated with cell-cycle regulation and carcinogenesis. A number of potential biomarkers of exposure, including translocations at codon 249 of TP53 in addition to HPRT mutations, have been suggested although, in conclusion, the evidence for such hotspots is insufficient. There is also substantial evidence of bystander effects, which may provide complications when calculating risk estimates as a result of exposure, particularly at low doses where cellular responses often appear to deviate from the linear, no-threshold hypothesis. At low doses, effects may also be dependent on cellular conditions as opposed to dose. The cellular and molecular carcinogenic effects of radon exposure have been observed to be both numerous and complex and the elevated chronic exposure of man may therefore pose a significant public health risk that may extend beyond the association with lung carcinogenesis.

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“…Similar trends were observed in studies of overexposed hospital workers by Bigatti et al [53], Jha and Sharma [54], and Barquinero et al [55]. Both Liu et al [56] and Thierens et al [57] compared the effectiveness of the standard CBMN assay to the DCA for estimating doses to individuals who were accidentally exposed to ionizing radiation. Both studies revealed a satisfactory agreement between the two methods, indicating that the CBMN assay may be used for the purposes of triage in cases of accidental overexposure where the dose must be estimated quickly.…”

Section: Manual Scoring Methodssupporting

confidence: 64%

Automation of the Cytokinesis-Block Micronucleus Assay Using Imaging Flow Cytometry for High Throughput Radiation Biodosimetry

Rodrigues¹

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The cytokinesis-block micronucleus (CBMN) assay is employed in biodosimetry to determine the dose of radiation to exposed individuals from the frequency of micronuclei (MN) in binucleated lymphocyte cells (BNCs). The assay is traditionally performed using a microscope-based scoring procedure that can be labour-intensive, time consuming and subject to variability of interpretation between scorers. This thesis investigates the x

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Dose estimation by chromosome aberration analysis and micronucleus assays in victims accidentally exposed to⁶⁰Co radiation

Abstract: ABSTRACT. The objective of this study was to assess the radiation exposure levels in victims of a 60

Cited by 27 publications

References 13 publications

Long-term health effects of persistent exposure to low-dose lr192 gamma-rays

Long-term health effects of persistent exposure to low-dose lr192 gamma-rays

The Cellular and Molecular Carcinogenic Effects of Radon Exposure: A Review

Automation of the Cytokinesis-Block Micronucleus Assay Using Imaging Flow Cytometry for High Throughput Radiation Biodosimetry

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Dose estimation by chromosome aberration analysis and micronucleus assays in victims accidentally exposed to60Co radiation

Abstract: ABSTRACT. The objective of this study was to assess the radiation exposure levels in victims of a 60

Cited by 27 publications

References 13 publications

Long-term health effects of persistent exposure to low-dose lr192 gamma-rays

Long-term health effects of persistent exposure to low-dose lr192 gamma-rays

The Cellular and Molecular Carcinogenic Effects of Radon Exposure: A Review

Automation of the Cytokinesis-Block Micronucleus Assay Using Imaging Flow Cytometry for High Throughput Radiation Biodosimetry

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Product

Resources

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Dose estimation by chromosome aberration analysis and micronucleus assays in victims accidentally exposed to⁶⁰Co radiation