Dose–effect relationships of nucleoplasmic bridges and complex nuclear anomalies in human peripheral lymphocytes exposed to⁶⁰Co γ-rays at a relatively low dose

Abstract

The dose effect between nucleoplasmic bridges (NPB) and relatively low doses of ionising radiation remains unknown. Accordingly, this study investigated the NPB frequencies in human peripheral blood lymphocytes exposed to low-dose (60)Co γ-rays. Complex anomalies, including fused nuclei (FUS), horse-shoe nuclei (HS) and circular nuclei (CIR), which possibly originated from multiple NPBs, were also scored. Human peripheral blood samples were collected from three healthy males and irradiated with 0-1 and 0-0.4 Gy (60)Co γ-rays. A cytokinesis-block micronucleus cytome assay was then conducted to analyse NPB, PFHC (NPB plus three complex nuclear anomalies) and micronucleus (MN) in binucleated cells. All dose-response curves followed the linear model for both NPB frequency and PFHC cell frequency. The dose-response curves between NPB frequency and absorbed dose at 0-1 and 0-0.4 Gy were y = 0.0037x + 0.0005 (R (2) = 0.979, P < 0.05) and y = 0.0043x + 0.0004 (R (2) = 0.941, P < 0.05), respectively. The dose-response curves between PFHC cell frequency and absorbed dose at 0-1 and 0-0.4 Gy were y = 0.0044x + 0.0007 (R (2) = 0.982, P < 0.05) and y = 0.0059x + 0.0005 (R (2) = 0.969, P < 0.05), respectively. The statistical significance of differences between the irradiated groups (0-0.4 Gy) and background levels of NPB, PFHC and MN were also analysed. The lowest analysable doses of NPB, PFHC and MN were 0.12, 0.08 and 0.08 Gy, respectively. In conclusion, NPBs and PFHC positively correlated with the absorbed radiation at a relatively low dose.

In vitro cytotoxic and genotoxic evaluation of peptides used in nuclear medicine (DOTATATE and Ubiquicidin29-41) in CHO-K1 cells

“…Apart from these goals, the method also would allow compatibility with image analysis, through manual or automatic acquisition, further expanding its capabilities. Metaphases, and mainly nucleoplasmic bridges, could be observed clearly in this study, and their detection could be profitably used in other studies as related by another study (Tian et al 2016).…”

Linear Dose Response of Acrocentric Chromosome Associations (Aca) To Gamma Irradiation In Human Lymphocytes

“…However, the DCA technique needs well-trained manpower and is time-consuming which has proved to be the main hindrance in its application [22][23][24]. In the case of MN, the analysis is in uenced by various factors and induced by several agents, thus not radiation speci c [34][35][36][37]. It is also important to record that dicentric aberrations are hardly induced by low radiation doses thus putting an additional constraint on the popular DCA method [38][39][40][41].…”

The Evaluation of Frequencies of Cytogenetic Biomarkers in Lymphocyte of Residents from High Natural Radiation Area in West Sulawesi, Indonesia

“…MN frequencies have increased by 23.99%, and NPB and NBUD due to this stimulant, showing that diesel engine exhaust level is related to this biomarker frequencies. At the same time, Tian et al [31] studied the relationship between NPB and a relatively low dose of 60 Co γ-rays in human peripheral blood lymphocytes. The lowest observable radiation doses of MN were 0.08, whereas and NPB were 0.08 Gy.…”