Dose-Dependent Tissue-Level Characterization of a Medical Atmospheric Pressure Argon Plasma Jet

Weiß, Martin; Barz, Jakob; Ackermann, Michael; Utz, Raphael; Ghoul, Aya; Weltmann, Klaus‐Dieter; Stope, Matthias B.; Wallwiener, D.; Schenke‐Layland, Katja; Oehr, Christian; Brucker, Sara Y.; Loskill, Peter

doi:10.1021/acsami.9b04803

Cited by 37 publications

(40 citation statements)

References 42 publications

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“…However, the induction of apoptosis was higher in CC cells compared to cells from healthy cervical tissue. The less significant apoptotic effect on non-cancerous cells is in line with an increasing number of studies utilizing APPJ, including our group [35,36]. Single cells were treated in suspension to avoid mechanical detachment and associated cell damage, as well as drying effects.…”

Section: Discussionmentioning

confidence: 61%

Trans-Mucosal Efficacy of Non-Thermal Plasma Treatment on Cervical Cancer Tissue and Human Cervix Uteri by a Next Generation Electrosurgical Argon Plasma Device

Wenzel

Berrio

Reisenauer

et al. 2020

Cancers

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Cancers 2020, 12, 267 2 of 16 velocity distribution, resulting in plasma temperatures being adjustable to body temperature [6][7][8]. Most importantly, the interaction of the reactive plasma factors with gaseous, liquid or solid substances is followed by the generation of reactive oxygen and nitrogen species (ROS and RNS), which is responsible for the induction of anti-proliferative cell mechanisms and cancer cell death [6][7][8][9][10]. To date, there are only few medically approved CAP devices. Thus, the investment and operation costs for these devices are relatively high. Similar to conventional CAP devices, the plasma effluent of non-thermally operated electrosurgical argon plasma sources contains diverse biologically reactive factors (charged particles and molecules, free radicals, ultraviolet, and infrared radiation) [11]. Commonly used electrosurgical argon plasma sources are devices for high-frequency (HF)-based surgery. These plasma sources are clinically well established and have been available for various clinical procedures for many years [12]. A great advantage of these devices is the variety of possible clinical applications using the highly flexible and sterile application probes in open and endoscopic surgery, which are associated with relatively low overheads. The system contains two electrodes. One electrode is active, and the other electrode is a neutral "ground", usually placed on the outer skin surface with proximity to the treated body region. The thermal effect during tissue coagulation is based on the contactless transfer of high-frequency alternating current from the plasma probe to the target tissue via ionized electrically conductive argon plasma, followed by energy conversion within the tissue (Joule heating) [13]. The argon plasma corridor thereby follows the way of lowest electrical resistance. The thermal effect is directly proportional to the electrical resistance of tissue and the square of amperage and can be avoided by using low energies and performing continuous motion of the plasma probe [11]. However, usually inducing thermal tissue effects, the ignited argon plasma of next generation electrosurgical argon plasma sources, as utilized in this study, is discussed to comprise lower energy per plasma particle as well as to combine characteristics of cold, non-equilibrium plasma, since thermal equilibrium within the plasma would require multiple actually used currents [14][15][16][17]. Due to the divergent principle of non-thermal plasma generation by electrosurgical argon plasma devices, we use the term non-invasive physical plasma (NIPP) treatment.Local NIPP treatment is a promising therapeutic procedure for various chronic inflammatory and neoplastic diseases of human mucosa, such as cervical intraepithelial neoplasia (CIN), where current treatment strategies are highly invasive, painful and associated with serious short-and long-term side effects and risks, especially during pregnancy [18][19][20][21][22][23][24].Very few in vitro studies have investigated the effects on cance...

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Section: Discussionmentioning

confidence: 61%

Trans-Mucosal Efficacy of Non-Thermal Plasma Treatment on Cervical Cancer Tissue and Human Cervix Uteri by a Next Generation Electrosurgical Argon Plasma Device

Wenzel

Berrio

Reisenauer

et al. 2020

Cancers

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“…Moreover, computer simulations showed that RNS reveal enhanced permeability through phospholipid bilayers compared to that of reactive oxygen species (ROS) [32]. Considering that elevated RONS levels overstrain the antioxidant resistance of cancer cells, MABS promises to be highly effective, as we found the reactivity of MABS significantly higher compared to the kINPen due to greater excitation of atoms measured by spatially resolved OES and higher levels of generated oxygen-centered radicals analyzed by ESR ( Figure 1) [19,21].…”

Section: Discussionmentioning

confidence: 77%

“…An increasing number of studies, however, reveal that healthy tissue is not as affected by apoptosis as critically as are malignant cells. Based on different apoptosis assays, our group recently showed that apoptosis cannot sufficiently explain the CAP-dependent inhibition of cell proliferation in primary human fibroblasts using an APPJ [19]. In line with this, Liedke et al showed similar apoptosis-independent cell effects on healthy mouse embryonic fibroblasts after treatment with an APPJ [28].…”

Section: Discussionmentioning

confidence: 89%

“…The generation of radicals within the gas, liquid, and solid interfaces are known to be the main triggers of CAP effects primarily linked to inhibition of cell proliferation and cell death [14][15][16]19]. Interestingly, by using electron spin resonance (ESR) spectroscopy, our research group recently showed that MABS was characterized by an 18-fold higher increase of total spin density generated within 10 s of treatment when compared to that of the CAP device kINPen med [19][20][21]. OH and H radicals significantly dominated the signals of other radicals in MABS-treated solutions, whereas superoxide anion radicals and hydroxyl radicals were the abundantly found reactive species in kINPen.…”

Section: Discussionmentioning

confidence: 99%

“…To characterize the spontaneous and induced emission spectra of MABS being emitted by excited species, we used conventional OES ( Figure 1d). Due to spectral differences depending on the axial position of the OES measurement within the MABS effluent, we additionally performed a spatially-resolved OES by using an integrating sphere [19] (Figure 1c,e). This enabled an absolute and uniform identification of MABS-induced emission spectra.…”

Section: Assessment Of a Non-thermally Operated Mabs By Infrared Thermentioning

confidence: 99%

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Cancer-Selective Treatment of Cancerous and Non-Cancerous Human Cervical Cell Models by a Non-Thermally Operated Electrosurgical Argon Plasma Device

Feil

Koch

Utz

et al. 2020

Cancers

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Cold atmospheric plasma (CAP) treatment is developing as a promising option for local anti-neoplastic treatment of dysplastic lesions and early intraepithelial cancer. Currently, high-frequency electrosurgical argon plasma sources are available and well established for clinical use. In this study, we investigated the effects of treatment with a non-thermally operated electrosurgical argon plasma source, a Martin Argon Plasma Beamer System (MABS), on cell proliferation and metabolism of a tissue panel of human cervical cancer cell lines as well as on non-cancerous primary cells of the cervix uteri. Similar to conventional CAP sources, we were able to show that MABS was capable of causing antiproliferative and cytotoxic effects on cervical squamous cell and adenocarcinoma as well as on non-neoplastic cervical tissue cells due to the generation of reactive species. Notably, neoplastic cells were more sensitive to the MABS treatment, suggesting a promising new and non-invasive application for in vivo treatment of precancerous and cancerous cervical lesions with non-thermally operated electrosurgical argon plasma sources.

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Little²,

Werkmann³

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Inkjet Printing in Industry

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Dose-Dependent Tissue-Level Characterization of a Medical Atmospheric Pressure Argon Plasma Jet

Cited by 37 publications

References 42 publications

Trans-Mucosal Efficacy of Non-Thermal Plasma Treatment on Cervical Cancer Tissue and Human Cervix Uteri by a Next Generation Electrosurgical Argon Plasma Device

Trans-Mucosal Efficacy of Non-Thermal Plasma Treatment on Cervical Cancer Tissue and Human Cervix Uteri by a Next Generation Electrosurgical Argon Plasma Device

Cancer-Selective Treatment of Cancerous and Non-Cancerous Human Cervical Cell Models by a Non-Thermally Operated Electrosurgical Argon Plasma Device

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