Dose-dependent effects of the antiprogestin, RU486, on sexual behavior of naturally cycling Fischer rats

Uphouse, Lynda

doi:10.1016/j.bbr.2015.01.004

Cited by 6 publications

(3 citation statements)

References 101 publications

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“…This latter point is especially interesting since RU486 did reduce proceptivity in naturally cycling proestrous rats (Uphouse, 2015). Although proestrous rats have a history of protracted exposure to endogenous estradiol (albeit at a lower dose than the 10 μg of the current study), these rats also have exposure to both estradiol and progesterone.…”

Section: 0 Discussionmentioning

confidence: 97%

Repeated estradiol benzoate treatment protects against the lordosis-inhibitory effects of restraint and prevents effects of the antiprogestin, RU486

Uphouse

Hiegel

Martinez

et al. 2015

Pharmacology Biochemistry and Behavior

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The following experiment was designed to test two specific questions: (1) Does the antiprogestin, RU486, reduce emergence of lordosis behavior and/or proceptivity in rats given repeated treatment with 10 μg estradiol benzoate (EB) and/or a single high dose (40 μg) of EB? (2) Does RU486 accentuate the effects of a 5 min restraint experience on sexual behaviors in rats given repeated treatment with estradiol benzoate (EB) and/or a high dose of EB? RU486 was used to determine if a high dose and/or repeated treatment with EB enhanced proceptivity and reduced the response to mild stress through an intracellular progesterone receptor-mediated process. Ovariectomized Fischer rats were injected with a single dose of 10 or 40 μg estradiol benzoate (EB) or received 4 consecutive weeks of treatment with 10 μg EB. Forty-eight hours after the last treatment with EB, rats were injected with 5 mg/kg of the antiprogestin, RU486, or the RU486 vehicle. That afternoon, rats were monitored for sexual behaviors. Sexually-receptive rats were then restrained for 5 min and again tested for sexual behaviors. A separate set of rats received 4 consecutive weeks of 10 μg EB treatment before treatment with a higher (5 mg/rat) dose of RU486. Lordosis to mount ratios, lordosis quality, proceptivity, and resistance were monitored. RU486 had no effect on the emergence of sexual behaviors but did accentuate the lordosis-inhibitory effect of restraint in rats given a single treatment with EB. Rats treated for 4 consecutive weeks with EB showed no effect of restraint and were unaffected by RU486. These findings lead to the suggestion that repeated EB initiates select behavioral effects that are not mimicked by acute EB treatment and that the intracellular progesterone receptor may not be involved.

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Section: 0 Discussionmentioning

confidence: 97%

Repeated estradiol benzoate treatment protects against the lordosis-inhibitory effects of restraint and prevents effects of the antiprogestin, RU486

Uphouse

Hiegel

Martinez

et al. 2015

Pharmacology Biochemistry and Behavior

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“…However, Hup‐A did not restore the plasma level of P 4 , which is mainly produced by the corpora lutea. Adequate levels of P4 in oestrogen‐primed rats facilitate proceptivity, sexual receptivity 34 and implantation of the fertilised egg 35 . Progesterone can be derived from non‐luteal as well as luteal sources in rats and guinea pigs 36,37 .…”

Section: Discussionmentioning

confidence: 99%

Huperzine‐A administration recovers rat ovary function after sympathetic stress

Riquelme

Ruz

Mayerhofer

et al. 2020

J Neuroendocrinology

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Chronic cold stress affects ovarian morphology and impairs fertility in rats. It causes an ovarian polycystic ovary (PCOS)‐like phenotype, which resembles PCOS in women. The mechanism of cold stress action involves increased ovarian noradrenaline (NA) levels, which remain elevated after cessation of cold stress. By contrast, ovarian acetylcholine (ACh) levels are only transiently elevated and returned to control levels after a 28‐day post stress period. Because ACh can exert trophic actions in the ovary, we hypothesised that a sustained elevation of ovarian ACh levels by intraovarian exposure to the ACh‐esterase blocker huperzine‐A (Hup‐A) may interfere with cold stress‐induced ovarian changes. This possibility was examined in female Sprague‐Dawley rats exposed to cold stress (4°C for 3 h day‐1 for 28 days), followed by a 28‐day period without stress. To elevate ACh, in a second group Hup‐A was delivered into the ovary of cold stress‐exposed rats. A third group was not exposed to cold stress. As expected, cold stress elevated ovarian NA, reduced the number of corpora lutea and increased the number of follicular cysts. It increased plasma testosterone and oestradiol but decreased plasma levels of progesterone. In the Hup‐A group, ovarian levels of both, NA and ACh, were elevated, there were fewer cysts and normal testosterone and oestradiol plasma levels were found. However, progesterone levels remained low. Most likely, low progesterone was associated with impaired mating behaviour and low pregnancy rate. We propose that elevated intraovarian levels of ACh are involved in the rescue of ovarian function, opening a target to control ovarian diseases affecting follicular development.

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“…Реализация основных компонентов репродуктивного поведения посредством эффектов прогестерона на уровне вентромедиального ядра гипоталамуса вовлекает в этот процесс нейроны ряда нейротрансмиттерных систем. Так, показано [98,99], что аллопрегнанолон, обладающий нейроактивным действием, способен увеличивать половую мотивацию, рецептивность, степень проявления реакции лордоза, что реализуется с участием дофамина и серотонина, которые лигандзависимым образом активируют ПР. Кроме того, прогестерон опосредованно регулирует половую активность, в том числе способствует женской восприимчивости за счет изменения синтеза оксида азота в ЦНС [100].…”

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A role of progesterone and its metabolites in regulation functions of the brain

Ivanova

Горобец

Litvinov

et al. 2018

Z. nevrol. psikhiatr. im. S.S. Korsakova

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The review presents literature data reflecting the nature and mechanism of the effect of progesterone and its metabolites on human and animal brain structures. Particular attention is paid to neuroprotective, anticonvulsant, anti-anxiety and sedative properties of this hormone, which determines the prospect of its use for the prevention and treatment of human neurodegenerative diseases, epilepsy, sleep disorders, and anxiety-depressive spectrum disorders, including premenstrual and climacteric syndromes.

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Dose-dependent effects of the antiprogestin, RU486, on sexual behavior of naturally cycling Fischer rats

Cited by 6 publications

References 101 publications

Repeated estradiol benzoate treatment protects against the lordosis-inhibitory effects of restraint and prevents effects of the antiprogestin, RU486

Repeated estradiol benzoate treatment protects against the lordosis-inhibitory effects of restraint and prevents effects of the antiprogestin, RU486

Huperzine‐A administration recovers rat ovary function after sympathetic stress

A role of progesterone and its metabolites in regulation functions of the brain

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