Dose-dependent effects of fenugreek seed extract on the biochemical and haematological parameters in high-fat diet-fed rats

Abstract

Objective The present study was carried out to assess the effects of fenugreek seed extract on various biochemical and haematological parameters in high-fat diet (HFD)-fed rats. Methods Female Wistar rats were allocated into five groups (n = 6): 1) control rats, 2) HFD-fed control rats 3) rats fed with HFD and fenugreek (FG) seed extract at doses of 200 mg/kg/day, 300 mg/kg/day, and 400 mg/kg/day for 12 weeks. Blood was collected to examine the biochemical and haematological parameters using a veterinary blood cell counter; blood indices such as MCV, MCH, MCHC, red blood cell distribution width, haemoglobin (Hb) levels, haematocrit, and platelet counts were measured. Blood samples were centrifuged at 3000 rpm for 10 min at room temperature to obtain serum for the estimation of lipid profiles, and aspartate transaminase and alanine transaminase levels. Results Rats fed with FG at a dose of 400 mg/kg/day showed a significant increase in the red blood cell count, Hb levels, haematocrit, and MCV, and a significant decrease in the lymphocyte count. The total cholesterol, triglyceride, and low-density lipoprotein levels increased significantly ( p < 0.05) in rats from the HFD control group, compared to those in the normal control group, but decreased significantly in rats fed with 400 mg/kg/day of FG. Conclusion The results of the current study suggest that FG seed extract exhibits hypolipidaemic activity and significantly improves the activity of hepatic enzymes, and the blood counts and indices in rats with HFD-induced obesity.

Modulation of inflammatory markers by petroleum ether fraction of Trigonella foenum‐graecum L. seed extract in ovariectomized rats

“…Fiber galactose and mannose being the main ingredients of the gum are also present in PE‐TFG seeds which help in reducing the lipid levels. A recent study published by the authors, rats fed with high‐fat diet for 12 weeks along with hydro‐alcoholic TFG seed extract in a dose‐dependent manner (200, 300, 400 mg kg –1 day –1 ), showed significantly decreased TC, TG levels with 400 mg kg –1 day –1 (Nagamma et al., 2019). A study focused on HMG‐CoA reductase inhibiting the effect of diosgenin.…”

“…The results are expressed as U/l. The oxidative stress marker, TBARS, and GSH, biomarkers of antioxidant defense were estimated as per our earlier reported methods (Nagamma et al., 2019).…”

Emvolio - A battery operated portable refrigerator preserves biochemical and haematological integrity of biological samples in preclinical studies

“…The blood samples were tested at a time interval of 3 hr intervals, 0 hr, 3 hr and 6 hrs, using the Veterinary blood cell counter (PCE-210VET, ERMA Inc., Tokyo, Japan). The blood samples were tested for total red blood cells (RBC), white blood cells (WBC), platelet, haemoglobin (Hb), haematocrit, mean corpuscular volume (MCV), mean corpuscular haemoglobin concentration (MCHC) and red cell distribution width (RDW) 11 . The temperature of each box was noted after the biological sample analysis was completed.…”

دور بذور الحلبة المنبتة کمغذیات فی الحمایة من السمیة الکلویة والکبدیة التی یحدثها السیسبلاتین للجرذان

“…However, it was associated with oxidative stress and lipid peroxidation as apparent by the increased level of ROS and MDA with a decreased level of total antioxidant activity. Nagamma et al (2019) reported that when decreasing the free radical-scavenging activity of the antioxidants, increased oxidative stress is noticed. El-Sayyad et al (2009) and Kart et al (2010) showed that Cis promoted extreme anomalies in liver tissue histology and the ultrastructure.…”

“…It has been well recognized to have many medicinal properties, such as anti-inflammatory, antidiabetic, antihyperlipidaemic, anticancer, antioxidant, as well neuroprotective activities. Also contains active components such as alkaloids, flavonoids (luteolin, kaempferol, quercetin, tricin, gallic acid… etc), steroids, and saponins (Sushma and Devasena, 2010, Tavakoly et al 2018, Nagamma et al, 2019.…”