Dose-dependent effect of triiodothyronine on the chondrogenic differentiation of mesenchymal stem cells from the bone marrow of female rats

Assis, Higor A; Elert, Nathalia C; Azevedo, André Luiz B P; Braga, Iolanda S; Serákides, Rogéria; Ocarino, Natália de Melo; Góes, Alfredo M.; Careta, Francisco de Paula; Paneto, Greiciane Gaburro; Silva, Adriana M A da; Boeloni, Jankerle Neves

doi:10.1111/jphp.12842

Cited by 6 publications

(7 citation statements)

References 72 publications

(115 reference statements)

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“…Before the BMSCs of this study were subjected to chondrogenic differentiation, cell viability was evaluated to ensure the continuity of the experiment and the differentiation process as performed by other previous studies (Alves et al, 2016;Assis et al, 2018). In this regard, both studied groups showed viability greater than 90% without a significant difference between these groups.…”

Section: Discussionmentioning

confidence: 94%

“…Cell morphology was assessed after 14 and 21 days of differentiation. Samples were washed with 0.15 M PBS and fixed in 10% buffered formaldehyde for one hour, processed by the routine paraffin embedding method (Assis et al, 2018;Solchaga et al, 2011). Subsequently, the samples were subjected to microtomy to obtain 4 µm thick sections and stained with Hematoxylin and Eosin for morphology analysis and stained with Periodic Schiff Acid (PAS) for the analysis of chondrogenic matrix formation.…”

Section: Animalsmentioning

confidence: 99%

“…Mesenchymal stem cells (MSC) can differentiate into several specific cell lineages, such as bone and cartilage (Kangari et al, 2020), being very important for regenerative medicine and for tissue engineering (Scibetta et al, 2019). Studies show that bone marrow mesenchymal stem cells (BMSCs) have the potential for chondrogenic differentiation (Assis et al, 2018), which allows for their use in the treatment of injuries or repairs of the cartilaginous tissue (Le et al, 2020). Some researchers have focused on what they call donor-to-donor MSC variation, source that include age, sex, species, and previous diseases (Kim et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the chondrogenic differentiation potential of bone marrow mesenchymal stem cells between female and male Wistar rats

Romão,

Tavares,

Peterle

et al. 2024

Arq. Bras. Med. Vet. Zootec.

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This study aimed to compare the chondrogenic differentiation potential between bone marrow mesenchymal stem cells from female (fBMSCs) and male (mBMSCs) Wistar rats. For this purpose, female and male BMSCs were subjected to chondrogenic differentiation for 7, 14 and 21 days for evaluation of cell morphology (14 and 21 days), formation of PAS+ chondrogenic matrix (21 days) and gene expression of aggrecan (Agg), collagen II (Col II) and Sox9 by RT-qPCR at 7, 14 and 21 days. Both fBMSCs and mBMSCs showed cell viability greater than 90%. After differentiation, the two groups showed morphologically similar cells at 14 and 21 days; PAS+ matrix formation was higher in fBMSCs when compared with mBMSCs. The Agg gene expression of mBMSCs was higher when compared with fBMSCs (p<0.05) at 21 days; however, the expression of Col II and Sox9 genes at 14 days was higher in fBMSCs compared to mBMSCs (p<0.05). Regarding the expression of genes over time, both in fBMSCs and in mBMSCs, the expression of Agg was lower at 21 days. BMSCs from female Wistar rats showed a greater chondrogenic differentiation potential compared to BMSCs from males.

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Section: Discussionmentioning

confidence: 94%

Section: Animalsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of the chondrogenic differentiation potential of bone marrow mesenchymal stem cells between female and male Wistar rats

Romão,

Tavares,

Peterle

et al. 2024

Arq. Bras. Med. Vet. Zootec.

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“…At the end of 21 days of culture, the chondrocyte pellets of all groups under 3D culture in 15 mL conical tubes were washed with 0.15 M PBS and fixed with 4% paraformaldehyde. The pellets were then processed into paraffin blocks 22 and subsequently sectioned into 5-μm slices using a microtome. The histological sections were placed under gelatinized glass slides 23 and stained by the periodic acid Schiff (PAS) (Sigma-Aldrich, St. Louis, MO, USA) to determine the percentage of PAS+ cartilaginous matrix.…”

Section: Methodsmentioning

confidence: 99%

Ethanol Alters Phenotype and Synthesis Activity of Rat Neonatal Articular Chondrocytes Grown in 2- and 3-Dimensional Culture

et al. 2019

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Objective We sought to evaluate the effect of different concentrations of ethanol on phenotype and activity of articular chondrocyte synthesis of neonatal rats in 2-dimensional (2D) and 3-dimensional (3D) culture. Methods Chondrocytes were cultured in chondrogenic medium with different concentrations of ethanol: 0.0% v/v (control); 0.05% v/v (8.6 mM); 0.25% v/v (42.9 mM), and 0.5% v/v (85.7 mM). Chondrocytes under 2D culture were subjected to MTT assay, while chondrocytes under 3D culture were processed for paraffin inclusion and stained by periodic acid Schiff (PAS) to evaluate mean chondrocyte diameter and percentages of cells, nucleus, cytoplasm, well-differentiated matrix, and PAS+ areas. The expression of gene transcripts for aggrecan, Sox9, and type II collagen was evaluated by real-time quantitative polymerase chain reaction. Results There was no difference between groups by the MTT assay. PAS staining revealed that chondrocytes treated with 0.5% v/v ethanol had higher percentages of cytoplasm and nuclear areas, but with a reduction in PAS+ matrix area. The mean diameter of chondrocytes was similar between groups. The expression of aggrecan in the group treated with 0.5% v/v ethanol was lower in comparison to that in the control. In the groups treated with 0.25% v/v and 0.5% v/v ethanol, the percentage of differentiated cartilage was lower in comparison with that in the control. The group treated with 0.05% v/v ethanol was similar to the control in all parameters. Conclusions Ethanol acted directly on in vitro cultured articular chondrocytes of newborn rats, altering the chondrocyte phenotype and its synthesis activity, and these effects were dose dependent.

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“…The role of thyroid hormones in stem cell function is still unclear and further study is required. Previous investigations have shown that the thyroid hormone T 3 exerts a dose-dependent effect on the chondrogenic and osteogenic differentiation of BMSC in female rats, promoting chondrogenic matrix formation and collagen synthesis respectively [22, 23]. T 3 treatment increases the differentiation of BMSCs induced to cardiomyocytes and promotes their maturation [24].…”

Section: Introductionmentioning

confidence: 99%

Combined ascorbic acid and T3 produce better healing compared to bone marrow mesenchymal stem cells in an Achilles tendon injury rat model: a proof of concept study

et al. 2019

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Background This pilot study aimed to ascertain whether the local application of ascorbic acid (AA), of T 3 , and of rat (r) bone marrow mesenchymal stem cells (BMSCs), alone or in all possible combinations, promoted healing after an Achilles tendon injury in a rat model. Methods An Achilles tendon defect was produced in 24 6–8-week-old male inbred Lewis rats. The animals were then randomly divided into eight groups of three rats each. The tendon defect was filled with 50 μL of phosphate-buffered saline (PBS) containing (1) 50 μg/mL AA (AA group), (2) 10 −7 M T 3 (T 3 group), (3) 4 × 10 6 rBMSCs (rBMSC group), (4) 50 μg/mL AA + 10 −7 M T 3 (AA + T 3 group), (5) 4 × 10 6 rBMSCs + 50 μg/mL AA (rBMSC + AA group), (6) 4 × 10 6 rBMSCs + 10 −7 M T 3 (rBMSC + T 3 group), (7) 4 × 10 6 rBMSCS + 50 μg/mL AA + 10 −7 M T 3 (rBMSC + AA + T 3 group), and (8) PBS only (control group: CTRL). All treatments were administered by local injection immediately after the tendons had been damaged; additionally, AA was injected also on the second and fourth day from the first injection (for groups 1, 4, 5, and 7), and T 3 was injected again every day for 4 days (for groups 2, 4, 6, and 7). At 30 days from initial treatment, tendon samples were harvested, and the quality of tendon repair was evaluated using histological and histomorphological analysis. The structure and morphology of the injured Achilles tendons were evaluated using the modified Svensson, Soslowsky, and Cook score, and the collagen type I and III ratio was calculated. Results The group treated with AA combined with T 3 displayed the lowest Svensson, Soslowsky, and Cook total score value of all tissue sections at histopathological examination, with fiber structure close to regular orientation, normal-like tendon vasculature, and no cartilage formation. AA + T 3 also showed the highest collagen I and the lowest collagen III values compared to all other treatments including the CTRL. Conclusion There are potential benefits using a combination of AA and T 3 to accelerate tendon healing.

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Dose-dependent effect of triiodothyronine on the chondrogenic differentiation of mesenchymal stem cells from the bone marrow of female rats

Cited by 6 publications

References 72 publications

Comparison of the chondrogenic differentiation potential of bone marrow mesenchymal stem cells between female and male Wistar rats

Comparison of the chondrogenic differentiation potential of bone marrow mesenchymal stem cells between female and male Wistar rats

Ethanol Alters Phenotype and Synthesis Activity of Rat Neonatal Articular Chondrocytes Grown in 2- and 3-Dimensional Culture

Combined ascorbic acid and T3 produce better healing compared to bone marrow mesenchymal stem cells in an Achilles tendon injury rat model: a proof of concept study

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