Dose-dependent effect of plasma-chemical NO-containing gas flow on wound healing. An experimental study

Shekhter, Anatoly B.; Пекшев, А. В.; Vagapov, A. B.; Butenko, Alexandra V.; Fayzullin, Alexey; Sharapov, Nikolay A.; Serejnikova, Natalia B.; Василец, В. Н.

doi:10.1016/j.cpme.2020.100101

Cited by 17 publications

(13 citation statements)

References 21 publications

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“…In contrast, other conditions foster an N 2 -driven chemistry, yielding species like ˙NO, ˙NO 2 , N 3 O 5 , HNO 2 , and HNO 3 [ 41 , 42 ]. The nitrogen species are assumed to play a role in bactericidal [ 43 , 44 ] and virucidal effects [ 45 ], as well as cell stimulation, e.g., in wound healing [ 11 , 46 , 47 , 48 , 49 , 50 , 51 , 52 ]. In contrast, data on their transport, solubilization, or production in liquids, as well as their impact on biological systems, are limited.…”

Section: Introductionmentioning

confidence: 99%

On the Liquid Chemistry of the Reactive Nitrogen Species Peroxynitrite and Nitrogen Dioxide Generated by Physical Plasmas

et al. 2020

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Cold physical plasmas modulate cellular redox signaling processes, leading to the evolution of a number of clinical applications in recent years. They are a source of small reactive species, including reactive nitrogen species (RNS). Wound healing is a major application and, as its physiology involves RNS signaling, a correlation between clinical effectiveness and the activity of plasma-derived RNS seems evident. To investigate the type and reactivity of plasma-derived RNS in aqueous systems, a model with tyrosine as a tracer was utilized. By high-resolution mass spectrometry, 26 different tyrosine derivatives including the physiologic nitrotyrosine were identified. The product pattern was distinctive in terms of plasma parameters, especially gas phase composition. By scavenger experiments and isotopic labelling, gaseous nitric dioxide radicals and liquid phase peroxynitrite ions were determined as dominant RNS. The presence of water molecules in the active plasma favored the generation of peroxynitrite. A pilot study, identifying RNS driven post-translational modifications of proteins in healing human wounds after the treatment with cold plasma (kINPen), demonstrated the presence of in vitro determined chemical pathways. The plasma-driven nitration and nitrosylation of tyrosine allows the conclusion that covalent modification of biomolecules by RNS contributes to the clinically observed impact of cold plasmas.

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Section: Introductionmentioning

confidence: 99%

On the Liquid Chemistry of the Reactive Nitrogen Species Peroxynitrite and Nitrogen Dioxide Generated by Physical Plasmas

et al. 2020

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“…450 and 492, Center for High Technologies in Machine Building, Bauman Moscow State Technical University. NO-containing gas flows were formed from atmospheric air by manipulators (plasmachemical nitric oxide generators), whose design was described in [41][42][43].…”

Section: Synthesis Of Binuclear Dnics With Glutathione and N-acetyl-l-cysteine Both B-dnic-gsh And B-dnic-nac Were Synthesized In A Thunbmentioning

confidence: 99%

“…In using the manipulator 2 NO C described in [42], variation of the distance from the outlet from 120 to 400 mm provided NO concentrations at the flow axis in the inhalation volume from 600 to 100 ppm, respectively; the temperature was within 50-20°C. With the manipulator described in [43], distance variation from 60 to 100 mm provided NO concentrations at the flow axis in the inhalation volume from 1000 to 2100 ppm; the temperature was within 50-25°C.…”

Section: Synthesis Of Binuclear Dnics With Glutathione and N-acetyl-l-cysteine Both B-dnic-gsh And B-dnic-nac Were Synthesized In A Thunbmentioning

confidence: 99%

“…This procedure was repeated nine times at nitric oxide concentrations from 100 to 2100 ppm. The NO uptake in the respiratory system at the respiration rate 12 breaths/min and inhaled volume 1 L/breath was calculated as (1) where and are expressed in ppm, and m, in mg/s [43].…”

Section: Synthesis Of Binuclear Dnics With Glutathione and N-acetyl-l-cysteine Both B-dnic-gsh And B-dnic-nac Were Synthesized In A Thunbmentioning

confidence: 99%

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Gaseous Nitric Oxide and Dinitrosyl Iron Complexes with Thiol-Containing Ligands as Potential Medicines that Can Relieve COVID-19

et al. 2021

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“…We also used Van Geison's staining to assess collagen production. The severity of the inflammatory reaction, number of fibroblasts and fat cells, and level of epithelization were examined using the Shekhter et al histological score table [32].…”

Section: Morphological Examinationmentioning

confidence: 99%

Cerium-Containing N-Acetyl-6-Aminohexanoic Acid Formulation Accelerates Wound Reparation in Diabetic Animals

et al. 2021

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Background: The main goal of our study was to explore the wound-healing property of a novel cerium-containing N-acethyl-6-aminohexanoate acid compound and determine key molecular targets of the compound mode of action in diabetic animals. Methods: Cerium N-acetyl-6-aminohexanoate (laboratory name LHT-8-17) as a 10 mg/mL aquatic spray was used as wound experimental topical therapy. LHT-8-17 toxicity was assessed in human skin epidermal cell culture using (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. A linear wound was reproduced in 18 outbred white rats with streptozotocin-induced (60 mg/kg i.p.) diabetes; planar cutaneous defect was modelled in 60 C57Bl6 mice with streptozotocin-induced (200 mg/kg i.p.) diabetes and 90 diabetic db/db mice. Firmness of the forming scar was assessed mechanically. Skin defect covering was histologically evaluated on days 5, 10, 15, and 20. Tissue TNF-α, IL-1β and IL-10 levels were determined by quantitative ELISA. Oxidative stress activity was detected by Fe-induced chemiluminescence. Ki-67 expression and CD34 cell positivity were assessed using immunohistochemistry. FGFR3 gene expression was detected by real-time PCR. LHT-8-17 anti-microbial potency was assessed in wound tissues contaminated by MRSA. Results: LHT-8-17 4 mg twice daily accelerated linear and planar wound healing in animals with type 1 and type 2 diabetes. The formulated topical application depressed tissue TNF-α, IL-1β, and oxidative reaction activity along with sustaining both the IL-10 concentration and antioxidant capacity. LHT-8-17 induced Ki-67 positivity of fibroblasts and pro-keratinocytes, upregulated FGFR3 gene expression, and increased tissue vascularization. The formulation possessed anti-microbial properties. Conclusions: The obtained results allow us to consider the formulation as a promising pharmacological agent for diabetic wound topical treatment.

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Dose-dependent effect of plasma-chemical NO-containing gas flow on wound healing. An experimental study

Cited by 17 publications

References 21 publications

On the Liquid Chemistry of the Reactive Nitrogen Species Peroxynitrite and Nitrogen Dioxide Generated by Physical Plasmas

On the Liquid Chemistry of the Reactive Nitrogen Species Peroxynitrite and Nitrogen Dioxide Generated by Physical Plasmas

Gaseous Nitric Oxide and Dinitrosyl Iron Complexes with Thiol-Containing Ligands as Potential Medicines that Can Relieve COVID-19

Cerium-Containing N-Acetyl-6-Aminohexanoic Acid Formulation Accelerates Wound Reparation in Diabetic Animals

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