Dose-dependent effect of adipose-derived adult stem cells on vertical bone regeneration in rabbit calvarium

Pieri, Francesco; Lucarelli, Enrico; Corinaldesi, Giuseppe; Aldini, N. Nicoli; Fini, Milena; Parrilli, Annapaola; Dozza, Barbara; Donati, Davide María; Marchetti, Claudio

doi:10.1016/j.biomaterials.2010.01.066

Cited by 66 publications

(62 citation statements)

References 33 publications

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“…Nevertheless MSC as well as ASC are currently in vitro expanded in order to obtain enough cells for most orthopaedic reconstructions. In particular in a preclinical study it has been demonstrated that vertical bone growth is dependent on the number of ASC implanted (6). In this study an increasing number of ASC were associated to 45 mg of anorganic bovine bone (ABB) and best results were obtained when 3xI07 ASC were implanted.…”

mentioning

confidence: 88%

Analysis of the Karyotype of Expanded Human Adipose-Derived Stem Cells for Bone Reconstruction of the Maxillo-Facial Region

Bellotti

Stanco

Ragazzini

et al. 2013

Int J Immunopathol Pharmacol

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Mesenchymal stem cells (MSC) and adipose-derived stem cells (ASC) were recently proposed for bone maxillofacial reconstruction in association with biomaterials. For this application MSC must be ex-vivo expanded in order to obtain, for a given volume of implanted biomaterial, a relevant number of bone forming cells. Previously conducted pre-clinical studies suggested that a concentration of 6 x 10 8 ASC associated with 900 mg of anorganic bovine bone (ABB) could be effective for human maxillary sinus floor elevation. A keystone issue to guarantee the quality and safety of Advanced Therapy Medicinal Products containing expanded MSC and ASC is their chromosome stability in culture: this topic has been widely investigated and conflicting results have been published. Abnormal karyotype of human ex-vivo expanded MSC and ASC was found by some authors, while, at the same time, several other studies showed the MSC and ASC karyotype to be normal. It is therefore important that all the results obtained on MSC andASC karyotype analysis be published. Given this context, the aim of this manuscript, aim of this manuscript is to verify the karyotype stability of ASC in view oftheir applications in clinical trials. ASC obtained from the adipose tissue of 4 donors were expanded over extended culture time. Based on previous ASC expansions we hypothesized to be able to obtain 6 x lOS cells by passage 7. Karyotype analysis 000 metaphases was planned to be investigated at passage 2, 7, and 15 in all the cultures. No abnormalities were found in the karyotype of two donors at all the passages tested, while a translocation was found in 2 metaphases of a donor at passage 7, but not at passage 15, and in the fourth donor in 5 metaphases a trisomy was found at passage 15. Chromosomal abnormalities were detected only after extended ASC expansion. Whether these anomalies can be related to risk for the patient's safety will have to be demonstrated by in-vivo studies.

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mentioning

confidence: 88%

Analysis of the Karyotype of Expanded Human Adipose-Derived Stem Cells for Bone Reconstruction of the Maxillo-Facial Region

Bellotti

Stanco

Ragazzini

et al. 2013

Int J Immunopathol Pharmacol

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“…The study of hASCs for bone regeneration has largely involved the insertion of biomaterials in rat and nude mouse models [88][89][90][91][92] . Furthermore, a femoral defect in nude rats is available and calvarial defect models have been described for other species, to demonstrate the application and optimization of hASCs in regenerative medicine [93][94][95][96][97] . However, relatively few reports are available concerning the utilization of hASCs for human bone tissue regeneration ( Table 1).…”

Section: Application Of Hascs and Scaffolds For Bone Tissue Engineeringmentioning

confidence: 99%

Adipose mesenchymal stem cells in the field of bone tissue engineering

Romagnoli

Brandi

2014

WJSC

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“…A dosedependent effect on bone formation has been reported for cultured ASCs applied to a calvarium rabbit bone defect. 57 ASCs promote healing of critical-sized cranial defects in mice and rabbits, [57][58][59][60] and have been successfully used in animal models for vertical bone regeneration, 57 as well as in MSFE using the SVF containing the ASCs 61 (Table 2). ASCs have been used successfully in humans to reconstruct major maxillary defects 22 and traumatic calvarial defects.…”

Section: Adipose Stem Cellsmentioning

confidence: 99%

Human Maxillary Sinus Floor Elevation as a Model for Bone Regeneration Enabling the Application of One-Step Surgical Procedures

Farré-Guasch

Prins

Overman

et al. 2013

Tissue Engineering Part B: Reviews

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Bone loss in the oral and maxillofacial region caused by trauma, tumors, congenital disorders, or degenerative diseases is a health care problem worldwide. To restore (reconstruct) these bone defects, human or animal bone grafts or alloplastic (synthetic) materials have been used. However, several disadvantages are associated with bone graft transplantation, such as limited bone volume, donor-site morbidity, surgical and immune rejection risks, and lack of osseo-integration. Bone tissue engineering is emerging as a valid alternative to treat bone defects allowing the regeneration of lost bony tissue, thereby recovering its functionality. During the last decades, the increasing aged population worldwide has also raised the prevalence of maxillary atrophy. Maxillary sinus floor elevation (MSFE) has become a standard surgical procedure to overcome the reduced amount of bone, thus enabling the placement of dental implants. MSFE aims to increase the bone height in the posterior maxilla, by elevating the Schneiderian membrane and placing the graft material into the surgically created space in the maxillary sinus floor. Importantly, oral bone regeneration during MSFE offers a unique human clinical model in which new cell-based bone tissue engineering applications might be investigated, since biopsies can be taken after MSFE before a dental implant placement and analyzed at the cellular level. New approaches in oral bone regeneration are focusing on cells, growth factors, and biomaterials. Recently, adipose tissue has become interesting as an abundant source of mesenchymal stem cells, which might be applied immediately after isolation to the patient allowing a one-step surgical procedure, thereby avoiding expensive cell culture procedures and another surgical operation. In this new cell-based tissue engineering approach, stem cells are combined with an osteoconductive scaffold and growth factors, and applied immediately to the patient. In this review, MSFE is discussed as a valid model to test bone tissue engineering approaches, such as the one-step surgical procedure. This procedure might be applied in other regenerative medicine applications as well.

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Dose-dependent effect of adipose-derived adult stem cells on vertical bone regeneration in rabbit calvarium

Cited by 66 publications

References 33 publications

Analysis of the Karyotype of Expanded Human Adipose-Derived Stem Cells for Bone Reconstruction of the Maxillo-Facial Region

Analysis of the Karyotype of Expanded Human Adipose-Derived Stem Cells for Bone Reconstruction of the Maxillo-Facial Region

Adipose mesenchymal stem cells in the field of bone tissue engineering

Human Maxillary Sinus Floor Elevation as a Model for Bone Regeneration Enabling the Application of One-Step Surgical Procedures

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