Dose-controlled exposure of A549 epithelial cells at the air–liquid interface to airborne ultrafine carbonaceous particles

Bitterle, Ellen; Karg, Erwin; Schroeppel, A.; Kreyling, Wolfgang G.; Tippe, A.; Ferron, G.A.; Schmid, Otmar; Heyder, J.; Maier, Konrad; Höfer, Thomas

doi:10.1016/j.chemosphere.2006.04.035

Cited by 133 publications

(114 citation statements)

References 35 publications

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“…Consequently, the cell-specific deposition efficiency is roughly half of the inner deposition efficiency, i.e. 35%, which is much greater than the collection efficiencies reported in the literature [16,17]. Comparing MEC with the previous in-house constructed exposure chamber (MAAPHRI version), the cell-specific collection efficiency of the current version has significantly increased by 120%, since in MAAPHRI version the inner deposition efficiency was 76%, but the ratio of growth area per the total surface of the chamber was only 21% (cell-specific collection efficiency 16%).…”

Section: Average 82%mentioning

confidence: 66%

“…In most of these techniques, the design is relying on diffusion and/or gravitational settling deposition mechanisms of the nanoparticles, as the aerosol flows directly to the cell culture surface by an inlet tube ending above it (basic configuration of commercial CULTEX® exposure system) using a stagnation point flow system [15]. Despite the advantageous operation compared with the liquid cover exposure, in this configuration the deposition efficiency of nanoparticles is typically low over a broad size range of 50 to 500 nm in (mobility) diameter; deposition efficiencies from 0.7% [16] to 2% [17] (for 200nm diameter particles) have been reported in the literature for systems with the aforementioned deposition mechanisms.…”

Section: Introductionmentioning

confidence: 99%

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Development of a dose-controlled multiculture cell exposure chamber for efficient delivery of airborne and engineered nanoparticles

Asimakopoulou

Daskalos

Lewinski

et al. 2013

J. Phys.: Conf. Ser.

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Section: Average 82%mentioning

confidence: 66%

Section: Introductionmentioning

confidence: 99%

Development of a dose-controlled multiculture cell exposure chamber for efficient delivery of airborne and engineered nanoparticles

Asimakopoulou

Daskalos

Lewinski

et al. 2013

J. Phys.: Conf. Ser.

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“…A commercially available perfusion unit (MINUCELL, Bad Abbach, Germany) was adapted to study the biological effects of fine and ultrafine particles on cells [87]. The radially symmetric stagnation point flow arrangement deposits particles uniformly and quantifiable onto a cell layer.…”

Section: Longtime Low-dose Deposition In Environmental Toxicologymentioning

confidence: 99%

Instrumented In Vitro Approaches to Assess Epithelial Permeability of Drugs from Pharmaceutical Formulations

Motz¹,

Bur²,

Schaefer³

et al.

Drug Absorption Studies

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The following chapter gives an overview of instrumented approaches to investigate the interactions of orally or pulmonary administered formulations with epithelial cell cultures in vitro. The first section is focused on the combined assessment of drug release/dissolution and subsequent absorption/permeation for solid oral dosage forms. Experimental approaches to mimic the complex physiologically surroundings in the gastrointestinal tract are presented as well as combinations of dissolution and permeation apparatus.The second part describes different experimental approaches to simultaneously assess deposition and subsequent absorption of pharmaceutical aerosol formulations, typically by adapting some existing impactor/impinger devices to accommodate pulmonary epithelial cell culture systems. Differences between longtime and low-dose aerosol deposition in environmental toxicology and short time bolus inhalation of pharmaceutical aerosols are elucidated.

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“…Ideally, ALI exposure systems must be able to deliver aerosol doses accurately over time, while keeping cell lines alive. Achieving this in practice presents a number of challenges, including determination of actual dose in contact with cells, resultant particle-size distribution, addressing perfusion and viability of cells, and acquisition of sufficient sample due to deposition efficiencies as low as 2% 159 .…”

Section: Lung-air Interface Systemsmentioning

confidence: 99%

“…These include the Cultex ® and Minucell ® systems 40,159 . Depending on requirements, the submersion of cells into the media and airflow parameters can be adjusted (see Figure 5).…”

Section: Lung-air Interface Systemsmentioning

confidence: 99%

In-vitro toxicology of respirable iron-rich particles relevant to mining industry: a high throughput framework for interpreting environmental particulate matter toxicity

Prakash¹

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This project investigated the cytotoxicity profiles and toxicological properties of inhalable iron-rich particles generated by mining activity. National Environment Protection Measure (NEPM) ambient air quality regulations specifically regulate sub-10 µm (PM10) and sub-2.5 µm (PM2.5) diameter particle fractions corresponding to thoracic and respirable lung penetration, respectively. However, atmospheric concentrations of fugitive iron-rich particulates in iron ore mining and processing associated settlements raised concerns that NEPM limits may inadequately address the specific toxicological profile of iron-rich PM10 and potentially adsorbed organics in these regions. To address this, comparative cytotoxicity experiments were carried out in-vitro using a variety of particulate standards, featuring other frequently studied metal-oxides, to help bridge in-vivo and epidemiological knowledge in the literature with in-vitro toxicological results. This also required the development of a novel framework to interpret physical and physiological characteristics of coarse, poorly soluble metal-oxide particles by conducting a critical systematic literature review.Analysis by the ChemCentre, WA was taken into account with relation to potentially absorbed organics and the influence of Polycyclic Aromatic Hydrocarbons (PAHs) investigated.The protocols developed by this project are applicable to high-throughput assessment of particulate matter generated by mining activity, having defined methodology to rapidly acquire sufficient quantities of PM10 for assays which can be multiplexed and the effects of particulate interference on assays minimised. This includes the integration of non-destructive sterilisation techniques, physical characterising by particle-sizing, particle solubility analysis and observation of particle morphology by electron microscopy and elemental analysis. Epithelial (A549), fibroblast (LL24) and monocyte differentiated macrophage (U937) cell lines were tested to build a picture of particulate effects on the major lung cell types in the lung parenchyma; the alveolar epithelium, connective tissue and immune system.The effect of lung fluid surfactants on cytotoxicity was determined, and advanced tissue culture methods were trialled to improve the differentiation between particles provided by the endpoints.ii The ore samples from the Mining Area C (MAC), Yandi and Newman Hub mines in the Pilbara region were found to have an effect comparable to the cytotoxicity of micron-size iron oxide standards and selected standards of titanium dioxide, silicon dioxide and carbon black, whereas copper oxide was found to have some ten-fold greater cytotoxicity across the cell lines. However cytotoxicity as a principle differentiator of toxicity was found to be insufficient in differentiating the physiological response to iron-rich PM10 and insoluble metal-oxide standards. At high concentrations the dose-response relationship plateaued at a maximum value for iron-oxide standards and iron ore samples in the A549 and LL2...

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Dose-controlled exposure of A549 epithelial cells at the air–liquid interface to airborne ultrafine carbonaceous particles

Cited by 133 publications

References 35 publications

Development of a dose-controlled multiculture cell exposure chamber for efficient delivery of airborne and engineered nanoparticles

Development of a dose-controlled multiculture cell exposure chamber for efficient delivery of airborne and engineered nanoparticles

Instrumented In Vitro Approaches to Assess Epithelial Permeability of Drugs from Pharmaceutical Formulations

In-vitro toxicology of respirable iron-rich particles relevant to mining industry: a high throughput framework for interpreting environmental particulate matter toxicity

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