Dose- and Time-Dependent Cellular Effects of Cold Atmospheric Pressure Plasma Evaluated in 3D Skin Models

Wiegand, Cornelia; Fink, Sarah; Beier, Oliver; Horn, Kerstin; Pfuch, Andreas; Schimanski, Arnd; Grünler, Bernd; Hipler, Uta‐Christina; Elsner, Peter

doi:10.1159/000450889

Cited by 37 publications

(30 citation statements)

References 30 publications

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“…[28] Cytokines and chemokines are the major regulators of inflammation in wound healing. [30] Similarly, Arndt reported that 2 min CAP treatment (microwave 2.45 GHz, 110 W, argon flow rate 4.0 L/min, treatment diameter 5 cm) increased the expression of some key genes of pro-inflammatory factors, such as IL-6, IL-8, MCP-1, TGF-1, and TGF-2, and improved wound healing. [20] Wiegand et al found that when CAP with air or nitrogen as the process gas is used, an increase in the expression of IL-6 and IL-8 (pro-inflammatory factor) occurred in a power-dependent (100-300 W) and time-dependent (5-15 s) manner in 3D skin models.…”

Section: Discussionmentioning

confidence: 94%

Effects and mechanisms of cold atmospheric plasma on skin wound healing of rats

Zhang

Guo

Chen

et al. 2018

Contributions to Plasma Physics

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Cold atmospheric plasma (CAP) has been reported as a novel potential way to promote skin wound healing. The aim of this study was to explore the effects of an atmospheric-pressure plasma generated in helium gas through dielectric barrier discharge on skin wound healing in rats and its mechanism. A total of 44 adult male Sprague-Dawley rats were divided into the CAP group and the control group after a full-thickness skin wound model was established. The animals in the CAP group received CAP treatment (driving frequency 17 kHz, root-mean-square [rms] values of the discharge voltage 1.33 kV, rms values of the discharge current 10.7 mA, helium flow rate 11 slpm [standard litres per minute]) for 1 min. After that, the size of the healed area of the wound, morphological changes in the wound, wound neovascularization, collagen deposition, and the level of inflammatory factors were recorded. The results showed that compared to that of the control group, the size of the wound area in the CAP group significantly decreased, the contraction and re-epithelialization of the wounds were obviously speeded up, and the protein level of smooth muscle actin markedly increased. However, the levels of collagen type I A2, collagen type III, and the deposition of collagen bundles did not change. In addition, it was found that the level of inflammatory factors (such as tumour necrosis factor and interleukin-1 ) significantly increased at 24 hr after the creation of the wound. These results suggest that CAP treatment in this condition could promote skin wound healing by accelerating the contraction, re-epithelialization, neovascularization, and inflammatory response.

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Section: Discussionmentioning

confidence: 94%

Effects and mechanisms of cold atmospheric plasma on skin wound healing of rats

Zhang

Guo

Chen

et al. 2018

Contributions to Plasma Physics

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“…By using a basic infection model, this pilot study provides a closer insight of pathogen–host interactions and the involvement of non‐professional cutaneous immune cells participating in the defence against the DSM 6916 strain of T. benhamiae . Follow‐up studies will use an infection model employing a human collagen‐based skin model that comprises fibroblasts building a dermis and keratinocytes generating the different strati of a human epidermis …”

Section: Discussionmentioning

confidence: 99%

“…Followup studies will use an infection model employing a human collagen-based skin model that comprises fibroblasts building a dermis and keratinocytes generating the different strati of a human epidermis. 65…”

Section: Discussionmentioning

confidence: 99%

Innate immune response of human epidermal keratinocytes and dermal fibroblasts to in vitro incubation of Trichophyton benhamiae DSM 6916

Hesse-Macabata

Morgner

Morgenstern

et al. 2019

Acad Dermatol Venereol

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Background Superficial cutaneous infection caused by the zoophilic dermatophyte Trichophyton benhamiae is often associated with a highly inflammatory immune response. As non‐professional immune cells, epidermal keratinocytes and dermal fibroblasts contribute to the first line of defence by producing pro‐inflammatory cytokines and antimicrobial peptides (AMP). Objective Purpose of this study was to gain a deeper understanding of the pathogenesis and the fungal–host interaction as not much is known about the innate immune response of these cutaneous cells against T. benhamiae. Methods Using a dermatophytosis model of fibroblasts and keratinocytes incubated with T. benhamiae DSM 6916, analyses included determination of cell viability and cytotoxicity, effects on the innate immune response including expression and secretion of pro‐inflammatory cytokines/chemokines and expression of AMP, as well as alterations of genes involved in cell adhesion. Results Trichophyton benhamiae DSM 6916 infection led to severe cell damage and direct induction of a broad spectrum of pro‐inflammatory cytokines and chemokines in both cutaneous cells. Only keratinocytes differentially up‐regulated AMP genes expression after T. benhamiae DSM 6916 infection. Expression of AMPs in fibroblasts was not inducible by fungal infection, whereas their absences potentially contributed to a continuous increase in the fungal biomass on fibroblasts, which in turn was reduced in keratinocytes possibly due to the antimicrobial actions of induced AMPs. On mRNA level, T. benhamiae DSM 6916 infection altered cell–cell contact proteins in keratinocytes, indicating that targeting specific cell–cell adhesion proteins might be part of dermatophytes’ virulence strategy. Conclusion This study showed that in addition to immune cells, keratinocytes and fibroblasts could participate in antimicrobial defence against an exemplary infection with T. benhamiae DSM 6916.

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“…The microbicidal effect of CAP was observed in vitro [256,257,258,259,260,261] on skin and chronic wounds, and exceeds the effectiveness of CHD, PVP-I, and PHMB. In a 3D epidermis model, CAP displayed dose- and time-dependent compatibility [256]; P. aeruginosa was inactivated without destroying the structure of the epidermis.…”

Section: Future Perspectivesmentioning

confidence: 99%

“…In a 3D epidermis model, CAP displayed dose- and time-dependent compatibility [256]; P. aeruginosa was inactivated without destroying the structure of the epidermis. Cell proliferation is supported in cell culture [247].…”

Section: Future Perspectivesmentioning

confidence: 99%

Consensus on Wound Antisepsis: Update 2018

Krämer

Dissemond²,

Kim³

et al. 2017

Skin Pharmacol Physiol

243

215

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Wound antisepsis has undergone a renaissance due to the introduction of highly effective wound-compatible antimicrobial agents and the spread of multidrug-resistant organisms (MDROs). However, a strict indication must be set for the application of these agents. An infected or critically colonized wound must be treated antiseptically. In addition, systemic antibiotic therapy is required in case the infection spreads. If applied preventively, the Wounds-at-Risk Score allows an assessment of the risk for infection and thus appropriateness of the indication. The content of this updated consensus recommendation still largely consists of discussing properties of octenidine dihydrochloride (OCT), polihexanide, and iodophores. The evaluations of hypochlorite, taurolidine, and silver ions have been updated. For critically colonized and infected chronic wounds as well as for burns, polihexanide is classified as the active agent of choice. The combination 0.1% OCT/phenoxyethanol (PE) solution is suitable for acute, contaminated, and traumatic wounds, including MRSA-colonized wounds due to its deep action. For chronic wounds, preparations with 0.05% OCT are preferable. For bite, stab/puncture, and gunshot wounds, polyvinylpyrrolidone (PVP)-iodine is the first choice, while polihexanide and hypochlorite are superior to PVP-iodine for the treatment of contaminated acute and chronic wounds. For the decolonization of wounds colonized or infected with MDROs, the combination of OCT/PE is preferred. For peritoneal rinsing or rinsing of other cavities with a lack of drainage potential as well as the risk of central nervous system exposure, hypochlorite is the superior active agent. Silver-sulfadiazine is classified as dispensable, while dyes, organic mercury compounds, and hydrogen peroxide alone are classified as obsolete. As promising prospects, acetic acid, the combination of negative pressure wound therapy with the instillation of antiseptics (NPWTi), and cold atmospheric plasma are also subjects of this assessment.

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Dose- and Time-Dependent Cellular Effects of Cold Atmospheric Pressure Plasma Evaluated in 3D Skin Models

Cited by 37 publications

References 30 publications

Effects and mechanisms of cold atmospheric plasma on skin wound healing of rats

Effects and mechanisms of cold atmospheric plasma on skin wound healing of rats

Innate immune response of human epidermal keratinocytes and dermal fibroblasts to in vitro incubation of Trichophyton benhamiae DSM 6916

Consensus on Wound Antisepsis: Update 2018

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