Dose and duration of nerve growth factor (NGF) administration determine the extent of behavioral recovery following peripheral nerve injury in the rat

Kemp, Stephen W.P.; Webb, Aubrey A.; Dhaliwal, S.K.; Syed, Shahbaz; Walsh, Sarah; Midha, Rajiv

doi:10.1016/j.expneurol.2011.03.017

Cited by 113 publications

(92 citation statements)

References 57 publications

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“…NTFs have been long studied for their positive effects on nerve regeneration, including their ability to promote neuronal survival, to increase axonal outgrowth and to improve target reinnervation. Different NTFs have been tested in vitro and in vivo to improve 4 peripheral nerve regeneration, such as nerve growth factor (NGF) (Kemp et al, 2011;Lee et al, 2003), brain-derived neurotrophic factor (BDNF) (Vögelin et al, 2006), neurotrophin-3 (NT-3) (Sternel et al, 1997), glial cell-derived neurotrophic factor (GDNF) (Moore et al, 2010), fibroblast growth factors (FGF1, FGF2) (Midha et al, 2003;Allodi et al, 2013) or insulin like growth factors (IGF1, IGF2) (Ishi et al, 1993;Kanje et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

Focal release of neurotrophic factors by biodegradable microspheres enhance motor and sensory axonal regeneration in vitro and in vivo

et al. 2016

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Neurotrophic factors (NTFs) promote nerve regeneration and neuronal survival after peripheral nerve injury. However, drawbacks related with administration and bioactivity during long periods limit their therapeutic application. In this study, PLGA microspheres (MPs) were used to locally release different NTFs and evaluate whether they accelerate axonal regeneration in comparison with free NTFs or controls. ELISA, SEM, UV/visible light microscopy, organotypic cultures of DRG explants and spinal cord slices were used to characterize MP properties and the bioactivity of the released NTFs. Results of organotypic cultures showed that encapsulated NTFs maintain longer bioactivity and enhance neurite regeneration of both sensory and motor neurons compared with free NTFs. For in vivo assays, the rat sciatic nerve was transected and repaired with a silicone tube filled with collagen gel or collagen mixed with PBS encapsulated MPs (control groups) and with free or encapsulated NGF, BDNF, GDNF or FGF-2. After 20 days, a retrotracer was applied to the regenerated nerve to quantify motor and sensory axonal regeneration. NTF encapsulation in MPs improved regeneration of both motor and sensory axons, as evidenced by increased numbers of retrolabeled neurons. Hence, our results show that slow release of NTFs with PLGA MP enhance nerve regeneration.

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Section: Introductionmentioning

confidence: 99%

Focal release of neurotrophic factors by biodegradable microspheres enhance motor and sensory axonal regeneration in vitro and in vivo

et al. 2016

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“…Present time points for the regeneration analysis were based on our earlier observations of peripheral nerve regeneration after transection injury. 19,20,47 In sharp contrast to the medium injection group, animals that received cell therapy showed by Week 10 clear signs of return to the normalgesic state according to a standard Hargreaves plantar test. The SKP-SC group showed maximum recovery potential, which was, however, closely followed by the nSC group.…”

Section: Discussionmentioning

confidence: 91%

“…All animals received an immediate entubulation repair within a silicon tube, inner diameter 1.5 mm, secured with 9-0 Prolene microsutures at each end, leaving a 5-mm gap between the proximal and distal stumps, using previously reported techniques. 19,38 Immediately following repair, 10 ml of nSCs (n = 6) or SKP-SCs (n = 5) or carrier medium (n = 6) was injected using a 10-ml Hamilton syringe with a 30-gauge needle into the segment of transected sciatic nerve immediately distal to the graft, giving a total of 1 × 10 6 cells. Schwann cells were labeled 20 minutes prior to injection with CellTracker CM-DiI (Invitrogen) according to the manufacturer's guidelines.…”

Section: Surgical Proceduresmentioning

confidence: 99%

Sensory recovery after cell therapy in peripheral nerve repair: effects of naïve and skin precursor-derived Schwann cells

Shakhbazau

Mohanty²,

Kumar

et al. 2014

JNS

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Object Cell therapy is a promising candidate among biological or technological innovations sought to augment microsurgical techniques in peripheral nerve repair. This report describes long-term functional regenerative effects of cell therapy in the rat injury model with a focus on sensory recovery. Methods Schwann cells were derived from isogenic nerve or skin precursor cells and injected into the transected and immediately repaired sciatic nerve distal to the injury site. Sensory recovery was assessed at weeks 4, 7, and 10. Axonal regeneration was assessed at Week 11. Results By Week 10, thermal sensitivity in cell therapy groups returned to a level indistinguishable from the baseline (p > 0.05). Immunohistochemistry at 11 weeks after injury showed improved regeneration of NF+ and IB4+ axons. Conclusions: The results of this study show that cell therapy significantly improves thermal sensation and the number of regenerated sensory neurons at 11 weeks after injury. These findings contribute to the view of skin-derived stem cells as a reliable source of Schwann cells with therapeutic potential for functional recovery in damaged peripheral nerve.

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“…In addition, augmentation of NGF enhances cholinergic neuronal markers and blockade of endogenous NGF impairs the retention of the spatial memory 35 . Administration of NGF is a significant determinant for the recovery of neural behavior 36 . Furthermore, mature NGF regulates neuronal cell survival via the binding of TrkaA and p75NTR receptors, and preform NGF promotes neuronal apoptosis also via the binding of p75NTR 37,38 .…”

Section: ■ Discussionmentioning

confidence: 99%

Effects of hyperbaric oxygen and nerve growth factor on the long-term neural behavior of neonatal rats with hypoxic ischemic brain damage

et al. 2017

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Effects of hyperbaric oxygen and nerve growth factor on the long-term neural behavior of neonatal rats with hypoxic ischemic brain damage 1Acta Cir. Bras. 2017;32(4):270-279 Abstract Purpose: To evaluate the effects of HBO (Hyperbaric oxygen) and NGF (Nerve growth factor) on the long-term neural behavior of neonatal rats with HIBD (Neonatal hypoxic ischemic brain damage). Methods:The HIBD model was produced by ligating the right common carotid artery of 7 days old SD (Sprague-Dawley) rats followed by 8% O2 + 92% N2 for 2h. Totally 40 rats were randomly divided into 5 groups including sham-operated group, HIBD control group, HBO treated group, NGF treated group and NGF + HBO treated group. The learning and memory ability of these rats was evaluated by Morris water maze at 30 days after birth, and sensory motor function was assessed by experiments of foot error and limb placement at 42 days after birth. Results:The escape latency of HBO treated group, NGF treated group and NGF + HBO treated group was shorter than that of HIBD control group (p<0.01) and longer than that of shamoperated group. The piercing indexes of 3 treated groups were higher than that of HIBD control group (p<0.01). Conclusion: Hyperbaric oxygen and nerve growth factor treatments may improve learning and memory ability and sensory motor function in neonatal rats after hypoxic ischemic brain damage. Key words: Hyperbaric Oxygenation. Nerve Growth Factor. Hypoxia-Ischemia, Brain. Rats. 2-Experimental Surgery 270Effects of hyperbaric oxygen and nerve growth factor on the long-term neural behavior of neonatal rats with hypoxic ischemic brain damage Wei L et. al. Acta Cir Bras. 2017;32(4):270-279 271 researchers have been performed about the roles of HBO and NGF in several aspects, but the effects of HBO and NGF on long-term neural behavior of neonatal rats have not been clearly explained.In the present study, we used SD (Sprague-Dawley) newborn rats to construct HIBD models. Furthermore, the experiments of place navigation, space search, feet errors and limb placement were made to explore learning and memory ability, and sensory motor function in neonatal rats after HIBD. We aimed to study the effects of HBO and NGF on the long-term neural behavior of neonatal rats with HIBD. ■ Methods Preparation of HIBD modelsAll procedures conform to the Principles of Laboratory Animal Care issued by the National Institues of Health, with local laws and regulations, and were approved by the local Animal Care and Use Committee.A total of 40 SD newborn rats of both sexes, weighing 14.5 ± 3.2g, aged 7 days old were selected and purchased from Medical Experimental Animal Center of Guangdong Province. The preparation process of HIBD models was followed as previously described 21 . SD rats were fixed in supine position, and routine disinfection of neck was performed. Subsequently, a 3 mm longitudinal incision was made, and the right common carotid artery was isolated by vessel forceps. The carotid artery was ligated by 0 sterile line, and then the incision was ...

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Dose and duration of nerve growth factor (NGF) administration determine the extent of behavioral recovery following peripheral nerve injury in the rat

Cited by 113 publications

References 57 publications

Focal release of neurotrophic factors by biodegradable microspheres enhance motor and sensory axonal regeneration in vitro and in vivo

Focal release of neurotrophic factors by biodegradable microspheres enhance motor and sensory axonal regeneration in vitro and in vivo

Sensory recovery after cell therapy in peripheral nerve repair: effects of naïve and skin precursor-derived Schwann cells

Effects of hyperbaric oxygen and nerve growth factor on the long-term neural behavior of neonatal rats with hypoxic ischemic brain damage

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