Dosage compensation of X-chromosome inactivation center-linked genes in porcine preimplantation embryos: Non-chromosome-wide initiation of X-chromosome inactivation in blastocysts

Hwang, Jae Yeon; Oh, Jong-Nam; Park, Chi-Hun; Lee, Dong‐Kyung; Lee, Chang Kyu

doi:10.1016/j.mod.2015.10.005

Cited by 15 publications

(22 citation statements)

References 39 publications

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“…This situation was thought to be comparable to other mammalian species, but transcriptional studies in bovine blastocysts refuted this notion, as most X-linked genes were upregulated in female blastocysts compared to their male counterparts (Bermejo-Alvarez et al 2010). This situation was also observed in rabbit and human blastocysts by conducting in situ hybridization (ISH) studies (Okamoto et al 2011), and in pigs, by transcriptional (Park et al 2012) and methylation analyses (Hwang et al 2015), so the mouse model was deemed to be more an exception than a rule for XCI dynamics (Bermejo-Alvarez et al 2012a). Indeed, bovine (Bermejo-Alvarez et al 2010) and rabbit (Okamoto et al 2011) embryos achieve extensive XCI later, around the time of gastrulation, which opens a large window when the upregulation of X-linked genes in female embryos occurs and provides a molecular basis for the appearance of sex-specific phenotypes.…”

Section: Sex Chromosomes Drive Sex Differences In the Absence Of Gonadsmentioning

confidence: 64%

Early sex-dependent differences in response to environmental stress

et al. 2018

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Developmental plasticity enables the appearance of long-term effects in offspring caused by exposure to environmental stressors during embryonic and foetal life. These long-term effects can be traced to pre-and post-implantation development, and in both cases, the effects are usually sex specific. During preimplantation development, male and female embryos exhibit an extensive transcriptional dimorphism mainly driven by incomplete X chromosome inactivation. These early developmental stages are crucial for the establishment of epigenetic marks that will be conserved throughout development, making it a particularly susceptible period for the appearance of long-term epigenetic-based phenotypes. Later in development, gonadal formation generates hormonal differences between the sexes, and male and female placentae exhibit different responses to environmental stressors. The maternal environment, including hormones and environmental insults during pregnancy, contributes to sex-specific placental development that controls genetic and epigenetic programming during foetal development, regulating sex-specific differences, including sex-specific epigenetic responses to environmental hazards, leading to long-term effects. This review summarizes several human and animal studies examining sex-specific responses to environmental stressors during both the periconception period (caused by differences in sex chromosome dosage) and placental development (caused by both sex chromosomes and hormones). The identification of relevant sex-dependent trajectories caused by sex chromosomes and/or sex hormones is essential to define diagnostic markers and prevention/intervention protocols.Reproduction (2018) 155 R39-R51

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Section: Sex Chromosomes Drive Sex Differences In the Absence Of Gonadsmentioning

confidence: 64%

Early sex-dependent differences in response to environmental stress

et al. 2018

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“…40 Diverse mechanisms exist in mammals with respect to the inactivation of XC in females. Generally, accumulation of H3K27me3 results in XC inactivation in females for dosage compensation.…”

Section: Discussionmentioning

confidence: 99%

“…40 In humans, XC dosage compensation has been reported in the preimplantation blastocyst but with conflicting mechanisms. This is consistent with the results of Hwang et al 40 They have reported that the dosage was compensated between the male and female blastocysts. 35 However, the other report concluded that, instead of XC inactivation, dosage compensation during human preimplantation development occurs by downregulation of both X chromsomes in females, a phenomenon named dampening of X expression.…”

Section: Discussionmentioning

confidence: 99%

Lineage specification and pluripotency revealed by transcriptome analysis from oocyte to blastocyst in pig

Kong

Zhang

et al. 2019

FASEB j.

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The inner cell mass (ICM) in blastocyst is the origin of all somatic and germ cells in mammals and pluripotent stem cells (PSCs) in vitro. As the conserved principles between pig and human, here we performed comprehensive single‐cell RNA‐seq for porcine early embryos from oocyte to early blastocyst (EB). We show the specification of the ICM and trophectoderm in morula and the molecular signature of the precursors. We demonstrate the existence of naïve pluripotency signature in morula and ICM of EB, and the specific pluripotent genes and the activity of signalling pathways highlight the characteristics of the naïve pluripotency. We observe the absence of dosage compensation with respect to X‐chromosome (XC) in morula, and incomplete dosage compensation in the EB. However, the dynamics of dosage compensation may be independent of the expression of XIST induced XC inactivation. Our study describes molecular landmarks of embryogenesis in pig that will provide a better strategy for derivation of porcine PSCs and improve research in regenerative medicine.

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“…Differences in the mechanisms of X-linked gene dosage compensation in female embryos are also evident 3 . The gene dosage compensation with respect to the X chromosomes in female embryos occurs in pre-gastrulation epiblasts in mouse and rabbits 3,8,16 . Notably, human post-implantation and pig pre-gastrulation epiblasts have not been studied 12,16 .…”

mentioning

confidence: 99%

Lineage segregation, pluripotency and X-chromosome inactivation in the pig pre-gastrulation embryo

Ramos‐Ibeas

Sang²,

Zhu

et al. 2018

Preprint

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5High-resolution molecular programs delineating the cellular foundations of and then of epiblast and hypoblast in late blastocysts. We detected distinct 3 3 pluripotent states, first as a short 'naïve' state followed by a protracted primed state. comprehending early human development and a foundation for further studies of 3 7 human pluripotent stem cell differentiation in pig interspecies chimeras. . CC-BY-NC-ND 4.0 International license It is made available under a (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint . http://dx.doi.org/10.1101/347823 doi: bioRxiv preprint first posted online Jun. 20, 2018; 3 Pre-gastrulation embryo development shows broad similarities between mammals, although 4 0 species-specific differences in early lineage segregation, the establishment of pluripotency, 4 1 and X chromosome inactivation have been reported [1][2][3] . Mouse embryos, which are widely 4 2 used as a model for mammalian development, transit rapidly through early development 4 3 (E3.5-E5.5, i.e. ~2 days), followed by development of the characteristic cup-shaped post- inaccessible, we are beginning to investigate relatively more accessible pig embryos. 8Notably both human and pig embryos evidently form a flat embryonic disc before the onset 4 9 of gastrulation 4 . Thus, the pig embryo can broaden our understanding of the pre-gastrulation 5 0development of large mammals with protracted development. 1Segregation of trophectoderm (TE) and hypoblast, and the emergence of pluripotency arewell established in mice 5,6 , but require detailed studies in other mammals at the resolution of lineage segregation have however emerged in reports between monkey and human, large mammalian species, are therefore highly desirable. 7In mouse embryos a distinct transcriptional signature of naïve pluripotency in the inner cell humans, has been challenging, suggesting possible biological differences 9,10 . Indeed, mouse embryonic naïve cells are not apparently detected in human embryos 9,11 . By contrast, . CC-BY-NC-ND 4.0 International license It is made available under a (which was not peer-reviewed) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.The copyright holder for this preprint . http://dx.doi.org/10.1101/347823 doi: bioRxiv preprint first posted online Jun. 20, 2018; 4 KLF17 is expressed in the human but not mouse ICM 9,11,12 . Also, while Jak-Stat3 and WNT 6 7 signalling are detected in the early mouse ICM 13 , many TGFβ signalling components are 6 8 present in marmoset, human and pig ICM 11,12,14,15 , indicating that the emergence and been studied 12,16 . 5Here we report lineage segregation, the establishment of pluripotency, and X-chromosomeinactivation during the entire peri-gastrulation period in the pig embryo using single cell RNA-seq (scRNA-seq). This comprehensive analysis provides new understanding of the the early human developmen...

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Dosage compensation of X-chromosome inactivation center-linked genes in porcine preimplantation embryos: Non-chromosome-wide initiation of X-chromosome inactivation in blastocysts

Cited by 15 publications

References 39 publications

Early sex-dependent differences in response to environmental stress

Early sex-dependent differences in response to environmental stress

Lineage specification and pluripotency revealed by transcriptome analysis from oocyte to blastocyst in pig

Lineage segregation, pluripotency and X-chromosome inactivation in the pig pre-gastrulation embryo

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