DoRWA3 from Dendrobium officinale Plays an Essential Role in Acetylation of Polysaccharides

Si, Can; Silva, Jaime A. Teixeira da; He, Chunmei; Yu, Zhenming; Zhao, Conghui; Wang, Haobin; Zhang, Mingze; Duan, Jun

doi:10.3390/ijms21176250

Cited by 12 publications

(5 citation statements)

References 59 publications

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“…To date, only two Dendrobium spp. genomes have been sequenced, and some candidate genes involved in polysaccharide metabolic pathways have been identified in those two species 24 , 29 , 30 . However, these studies were largely limited due to their low-quality genome assemblies.…”

Section: Introductionmentioning

confidence: 99%

Chromosome-scale assembly of the Dendrobium chrysotoxum genome enhances the understanding of orchid evolution

Zhang

et al. 2021

Hortic Res

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As one of the largest families of angiosperms, the Orchidaceae family is diverse. Dendrobium represents the second largest genus of the Orchidaceae. However, an assembled high-quality genome of species in this genus is lacking. Here, we report a chromosome-scale reference genome of Dendrobium chrysotoxum, an important ornamental and medicinal orchid species. The assembled genome size of D. chrysotoxum was 1.37 Gb, with a contig N50 value of 1.54 Mb. Of the sequences, 95.75% were anchored to 19 pseudochromosomes. There were 30,044 genes predicted in the D. chrysotoxum genome. Two whole-genome polyploidization events occurred in D. chrysotoxum. In terms of the second event, whole-genome duplication (WGD) was also found to have occurred in other Orchidaceae members, which diverged mainly via gene loss immediately after the WGD event occurred; the first duplication was found to have occurred in most monocots (tau event). We identified sugar transporter (SWEET) gene family expansion, which might be related to the abundant medicinal compounds and fleshy stems of D. chrysotoxum. MADS-box genes were identified in D. chrysotoxum, as well as members of TPS and Hsp90 gene families, which are associated with resistance, which may contribute to the adaptive evolution of orchids. We also investigated the interplay among carotenoid, ABA, and ethylene biosynthesis in D. chrysotoxum to elucidate the regulatory mechanisms of the short flowering period of orchids with yellow flowers. The reference D. chrysotoxum genome will provide important insights for further research on medicinal active ingredients and breeding and enhances the understanding of orchid evolution.

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Section: Introductionmentioning

confidence: 99%

Chromosome-scale assembly of the Dendrobium chrysotoxum genome enhances the understanding of orchid evolution

Zhang

et al. 2021

Hortic Res

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“…The RWA protein family regulates cell wall acetylation after xylan synthesis and is required for effective xylan binding to cellulose [ 23 ]. The overexpression of RWA3 in Dendrobium officinale significantly increased polysaccharides’ acetylation level in seeds, leaves, and stems [ 24 ]. PG is involved in carbohydrate metabolism and is very important for cell wall metabolism and the maintenance of plant tissue morphology.…”

Section: Discussionmentioning

confidence: 99%

Identification of a Comprehensive Gene Co-Expression Network Associated with Autotetraploid Potato (Solanum tuberosum L.) Development Using WGCNA Analysis

Li,

Wang,

Wang

2023

Genes

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The formation and development of potato tissues and organs is a complex process regulated by a variety of genes and environmental factors. The regulatory mechanisms underlying the growth and development are still unclear. In this work, we aimed to explore the changes in gene expression patterns and genetic characteristics of potato tissues throughout different developmental stages. To achieve this, we used autotetraploid potato JC14 as an experimental subject to analyze the transcriptome of the root, stem, and leaf at the seedling, tuber formation, and tuber expansion stages. The results revealed thousands of differentially expressed genes, predominantly involved in defense response and carbohydrate metabolism according to KEGG pathway enrichment analysis. Weighted gene co-expression network analysis (WGCNA) revealed a total of 12 co-expressed gene modules, with 4 modules showing the highest correlation with potato stem development. By calculating the connectivity of genes within the module, hub genes were identified, and functional annotations were subsequently performed. A total of 40 hub genes from the four modules were identified, and their functions were found to be related to carbohydrate metabolism, defense response, and transcription factors. These findings provide important insights for further understanding of the molecular regulation and genetic mechanisms involved in potato tissue development.

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“…RWA protein family plays an important role in regulating cell wall acetylation after xylan synthesis and are essential for the efficient binding of xylan to cellulose [23]. Over-expression of Dendrobium officinale RWA3 significantly increased the acetylation level of polysaccharides in seeds, leaves and stems [24]. PG is involved in carbohydrate metabolism and is very important for cell wall metabolism and maintenance of plant tissue morphology.…”

Section: Discussionmentioning

confidence: 99%

WGCNA Identifies a Comprehensive Gene Co-Expression Network Associated with Development in autotetraploid potato tissues

Li¹,

Wang²,

Wang³

2023

Preprint

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The formation and development of potato tissues and organs is a complex process regulated by a variety of genes and environmental factors. However, the regulatory mechanisms underlying the growth and development are still unclear. In this study, we used autotetraploid potato JC14 as experimental subject to analyze the transcriptome of root, stem and leaf at seedling, tuber formation and tuber expansion stages to explore the spatio-temporal expression pattern of genes and genetic development characteristics. The results identified thousands of differentially expressed genes and KEGG pathway enrichment analysis showed that these genes were mainly involved in defense response and carbohydrate metabolism pathways. A total of 12 co-expressed Gene modules were identified by Weighted Gene Co-expression Network Analysis (WGCNA), and 4 modules were screened out with the highest correlation with potato stem developmental traits. Core genes in the network were further investigated and functionally annotated by computing the connectivity of genes within the module. The results unveiled number of hub genes in stems at different developmental stages, including carbohydrate metabolism related genes, the defense response related genes, and transcription factors. These findings provide important leads for further understanding of the molecular regulation and genetic mechanisms of potato tissue development.

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DoRWA3 from Dendrobium officinale Plays an Essential Role in Acetylation of Polysaccharides

Cited by 12 publications

References 59 publications

Chromosome-scale assembly of the Dendrobium chrysotoxum genome enhances the understanding of orchid evolution

Chromosome-scale assembly of the Dendrobium chrysotoxum genome enhances the understanding of orchid evolution

Identification of a Comprehensive Gene Co-Expression Network Associated with Autotetraploid Potato (Solanum tuberosum L.) Development Using WGCNA Analysis

WGCNA Identifies a Comprehensive Gene Co-Expression Network Associated with Development in autotetraploid potato tissues

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