Dorsal aorta catheterization in rainbow trout (Salmo gairdneri). I. Its validity in the study of blood gonadotropin patterns

Zohar, Yonathan

doi:10.1051/rnd:19801007

Cited by 39 publications

(23 citation statements)

References 20 publications

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“…Animals were cannulated according to the technique of Zohar (1980) and allowed to recover for five days. The fish were not fed on the day of the experiment and blood (200 ld) was obtained each hour.…”

Section: In Vivo Experimentsmentioning

confidence: 99%

Development of a protein binding assay for teleost insulin-like growth factor (IGF)-like: relationships between growth hormone (GH) and IGF-like in the blood of rainbow trout (Oncorhynchus mykiss)

Niu

Pérez‐Sanchez

Bail

1993

Fish Physiol Biochem

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Using rainbow trout plasma protein (IGF-BP) which specifically binds human insulin-like growth factor (IGF) (Niu and Le Bail 1993), we have developed an assay to measure plasma IGF-like levels in different teleost species. Before the assay and to prevent interference by IGF-BP, IGF-like was extracted from all samples, using SP Sephadex C-25 in acidic conditions. After this treatment, contamination of the IGF fraction by IGF-BP which was estimated by binding assay, was approximately 5%, and was not detectable by western ligand blot.Human IGF-I was used as standard and labelled hormone. Sensitivity of the assay was 0.15-0.40 ng/ml (ED90) and ED50 was 1-3 ng/ml. hIGF-II crossreaction was partial and no significant displacement was observed with Insulin from different species or with other hormones. Inhibition curves were obtained with plasma IGF fractions (but not with tissue extracts) from teleost and mammals and are parallel to the standard curve. These results suggest that the protein binding assay can quantify an IGF-like factor in the plasma of teleost and that the binding sites of IGF are well conserved during vertebrate evolution.Using this IGF binding assay, IGF-like was measured in parallel with growth hormone (GH) in plasma from young rainbow trout killed every 1.5h throughout one day. The daily profiles for both hormones, which appear pulsatile, are similar. A significant correlation was observed between GH levels and IGF-like levels with a 1.5h delay. Analogous observations were obtained in individual catheterized adult rainbow trout. Although plasma GH levels differ greatly between fish, less variability is found with IGF-like. In a third experiment, rainbow trout were starved or submitted to bovine GH treatment for four weeks. Starved fish, in which plasma GH levels increased, had plasma IGF-like level significantly lower than in fed fish. In bGH injected fish, plasma IGF-like level was significantly higher than in non-injected fish. These results suggest that, as in mammals, IGF-like secretion depends on plasma GH level and could be modulated by the nutritional status of fish.

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Section: In Vivo Experimentsmentioning

confidence: 99%

Development of a protein binding assay for teleost insulin-like growth factor (IGF)-like: relationships between growth hormone (GH) and IGF-like in the blood of rainbow trout (Oncorhynchus mykiss)

Niu

Pérez‐Sanchez

Bail

1993

Fish Physiol Biochem

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“…The technique allows repeated blood sampling from the same fish compared to the alternative of serial slaughter and was first described by Smith and Bell (1964). Most studies that have utilized this method have focused on short-term physiological measurements such as blood volume (Conte et al 1963), evaluation of haematological parameters (Soivio et al 1975), endocrinology (Zohar 1980), post-prandial levels of plasma amino acids (Ok et al 2001), degree and time course of changes in catecholamine and cortisol concentrations in cannulated black box-confined rainbow trout (Gamperl et al 1994a) and immunological measures (Lo et al 2003). In a series of experiment, Kiessling et al introduced long-term studies of free swimming and voluntarily feeding cannulated fish (Eliason et al 2007(Eliason et al , 2009Hamre et al 2001;Karlsson et al 2006;Kiessling et al 1995Kiessling et al , 2003Kiessling et al , 2006Kiessling et al , 2009Olsen et al 2005a;Sunde et al 2003) The DA cannulation method involves several procedures that are known to stress the fish, such as handling, sedation and surgery, in addition to isolating the fish in individual tanks.…”

Section: Introductionmentioning

confidence: 99%

Effect of nutritional status and sampling intensity on recovery after dorsal aorta cannulation in free-swimming Atlantic salmon (Salmo salar L.)

Djordjevic

Kristensen

Øverli

et al. 2009

Fish Physiol Biochem

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Recovery from implantation of a cannula in the dorsal aorta (DA) of Atlantic salmon (Salmo salar) was studied in relation to nutritional status and sampling intensity. The incentive for the study was the inconsistency between published reports and our own experience of recovery and longevity of fish exposed to this protocol. In two studies using starved fish, blood (0.3 ml) was sampled 0, 1 and 24 h after DA-cannulation, and thereafter at 48 and 72 h and thereafter once weekly for four weeks. In a third study using fed fish, four consecutive samples (0, 3, 6 and 12 h after a meal) were obtained twice a week over a four-week period. All fish displayed a sharp increase in pCO(2) and haematocrit (Hct) during surgery, followed by a marked raise in cortisol, glucose, sodium and potassium (1 h). pCO(2), pH and Hct approached baseline levels as early as the 1 h post-surgery sample, while this was not the case for cortisol and electrolytes before the 24 h post-surgery sample. Glucose did not display any significant changes post surgery. From then on, all variables displayed minor but non-significant (P > 0.05) changes indicating a steady state close to baseline values for unstressed fish. This pattern was independent of sampling procedure, i.e. repeated single or multiple samples and thus volume of blood removed. Nutritional status (fed vs. starved) did not affect post-surgical recovery pattern. Only K(+) and Hct displayed consistent and significant post-prandial patterns. We found marked differences between baseline level of cannulated fish and uncannulated control fish, in pH, K(+) and Hct indicating that cannulation may be the preferred method to obtain representative resting values in fish.

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“…Fish dorsal aorta cannulation procedures were performed according to Zohar (Zohar, 1980), allowing recovery from surgery for 24h under free-swimming conditions. Trout were fasted for 48 h and then subjected to four different experiments involving injection of solutions through the cannula into the dorsal aorta: (i) glucose injection (250 mg kg -1 ), (ii) glucose plus bovine insulin (75 mIU kg -1 or ~2.7 mg kg -1 , Sigma, St Louis, MO, USA) injection, (iii) glucose injection 15 min (peak of glucose in plasma) after the first glucose administration, and (iv) glucose plus insulin injection 15 min after the first glucose administration.…”

Section: Experimental Protocolsmentioning

confidence: 99%

Insulin-induced hypoglycaemia is co-ordinately regulated by liver and muscle during acute and chronic insulin stimulation in rainbow trout (Oncorhynchus mykiss)

Polakof

Skiba‐Cassy

Choubert

et al. 2010

Journal of Experimental Biology

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SUMMARYThe relative glucose intolerance of carnivorous fish species is often proposed to be a result of poor peripheral insulin action or possibly insulin resistance. In the present study, data from aortic cannulated rainbow trout receiving bovine insulin (75 mIU kg -1 ) injections show for the first time their ability to clear glucose in a very efficient manner. In another set of experiments, mRNA transcripts and protein phosphorylation status of proteins controlling glycaemia and glucose-related metabolism were studied during both acute and chronic treatment with bovine insulin. Our results show that fasted rainbow trout are well adapted at the molecular level to respond to increases in circulating insulin levels, and that this hormone is able to potentially improve glucose distribution and uptake by peripheral tissues. After acute insulin administration we found that to counter-regulate the insulininduced hypoglycaemia, trout metabolism is strongly modified. This short-term, efficient response to hypoglycaemia includes a rapid, coordinated response involving the reorganization of muscle and liver metabolism. During chronic insulin treatment some of the functions traditionally attributed to insulin actions in mammals were observed, including increased mRNA levels of glucose transporters and glycogen storage (primarily in the muscle) as well as decreased mRNA levels of enzymes involved in de novo glucose production (in the liver). Finally, we show that the rainbow trout demonstrates most of the classic metabolic adjustments employed by mammals to efficiently utilize glucose in the appropriate insulin context.

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Dorsal aorta catheterization in rainbow trout (Salmo gairdneri). I. Its validity in the study of blood gonadotropin patterns

Abstract: Introduction.

Cited by 39 publications

References 20 publications

Development of a protein binding assay for teleost insulin-like growth factor (IGF)-like: relationships between growth hormone (GH) and IGF-like in the blood of rainbow trout (Oncorhynchus mykiss)

Development of a protein binding assay for teleost insulin-like growth factor (IGF)-like: relationships between growth hormone (GH) and IGF-like in the blood of rainbow trout (Oncorhynchus mykiss)

Effect of nutritional status and sampling intensity on recovery after dorsal aorta cannulation in free-swimming Atlantic salmon (Salmo salar L.)

Insulin-induced hypoglycaemia is co-ordinately regulated by liver and muscle during acute and chronic insulin stimulation in rainbow trout (Oncorhynchus mykiss)

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