Dormancy release of Cotinus coggygria seeds under a pre-cold moist stratification: an endogenous abscisic acid/gibberellic acid and comparative proteomic analysis

Deng, Z. J.; Hu, Xiao; Ai, Xun R.; Yao, Lan; Deng, Shiming; Pu, Xin; Song, Song Quan

doi:10.1007/s11056-015-9496-2

Cited by 7 publications

(5 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…GA stimulates seed germination, and ABA initializes seed dormancy (Bewley 1997;Chen et al 2008;Miransari and Smith 2014;Deng et al 2016;Guo et al 2020). However, the variations in GA 3 levels did not parallel the G P or G V patterns in response to light or temperature, which indicates that GA did not act as the stimulator for P. koraiensis germination in this study.…”

Section: Discussioncontrasting

confidence: 55%

The coupled effect of light and temperature on dormancy release and germination of Pinus koraiensis seeds

Zhang

Zhu

2021

J. For. Res.

View full text Add to dashboard Cite

Elucidating the regulatory mechanisms of environmental factors on seed dormancy and germination will provide guidance for tree regeneration. Toward understanding the coupled effect of light and temperature on dormancy release and germination of Pinus koraiensis seeds, we set up three light conditions (L200: 200 μmol m−2 s−1, L20: 20 μmol m−2 s−1, L0: 0 μ m−2 s−1) and four storage temperatures [T-5: − 5 °C (50 days), T5: − 5 °C (50 days) + 5 °C (50 days), T25: − 5 °C(50 days) + 5 °C (50 days) + 25 °C (50 days), T15: − 5 °C (50 days) + 5 °C (50 days) + 25 °C (50 days) + 15 °C (50 days)] using imbibed seeds, then quantified phytohormones gibberellic acid (GA3) and abscisic acid (ABA) during the stratification. Germination percentage (GP), mean germination time (TM), and germination value (GV) under 25/15 °C temperature and the three light conditions were then determined. Phytohormone levels and germination performances were significantly affected by light and temperature. No consistent trend was found between the phytohormone levels and GP caused by light levels. Under the three light conditions, ABA concentrations in the embryo and endosperm decreased as storage temperature shifted from T-5 to T25 and increased from T25 to T15; GA3 decreased in nearly all four storage temperatures. GP reached 40–60% in T25 storage without light irradiance. In the three light conditions, GP and GV were higher at T5 and T25 than at T-5 and T15; so T5 and T25 are considered as optimum storage temperatures for dormancy release and germination. At optimum temperatures, light (L200, L20) significantly increased the GP and GV compared with the dark (L0). At L200 and L20, significant negative correlations between GV and the ABA concentrations and positive correlations between GV and GA/ABA in the seed embryo were found. Temperature played a more important role in primary dormancy release and germination; light was unnecessary for primary dormancy release. Light facilitated seed germination at optimum temperatures. The dormancy release and germination of P. koraiensis seeds were controlled by a decrease in ABA concentrations or an increase in GA/ABA induced by temperature variations.

show abstract

Section: Discussioncontrasting

confidence: 55%

The coupled effect of light and temperature on dormancy release and germination of Pinus koraiensis seeds

Zhang

Zhu

2021

J. For. Res.

View full text Add to dashboard Cite

show abstract

“…Accumulation of actin-7, as well as legumin, corresponded with the reserve deposition phase in Medicago truncatula seeds [ 39 ]. Actins, including actin-7, play an essential role in germination and root growth [ 40 , 41 , 42 , 43 , 44 ]. Actin-depolymerizing factor 2 (ADF2) proteins involved in a dynamic change in the cytoskeleton necessary for embryo cell elongation increased in abundance in germinated seeds but not in ungerminated thermoinhibited seeds [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

“…The expression trend of the ATP synthase beta subunit showed an up-regulated pattern and demonstrated that energy metabolism continuously bolstered the process of germination [ 55 ]. The increase in activity of ATPases was observed during dormancy breaking of several tree seeds caused by cold stratification [ 40 , 56 , 57 , 58 ]. The present study demonstrated that the ATPase alpha subunit was up-accumulated during dormancy release of rose seeds, but this was observed only during warm stratification.…”

Section: Resultsmentioning

confidence: 99%

Temperature Regulation of Primary and Secondary Seed Dormancy in Rosa canina L.: Findings from Proteomic Analysis

Pawłowski

Bujarska-Borkowska

Suszka

et al. 2020

IJMS

View full text Add to dashboard Cite

Temperature is a key environmental factor restricting seed germination. Rose (Rosa canina L.) seeds are characterized by physical/physiological dormancy, which is broken during warm, followed by cold stratification. Exposing pretreated seeds to 20 °C resulted in the induction of secondary dormancy. The aim of this study was to identify and functionally characterize the proteins associated with dormancy control of rose seeds. Proteins from primary dormant, after warm and cold stratification (nondormant), and secondary dormant seeds were analyzed using 2-D electrophoresis. Proteins that varied in abundance were identified by mass spectrometry. Results showed that cold stratifications affected the variability of the highest number of spots, and there were more common spots with secondary dormancy than with warm stratification. The increase of mitochondrial proteins and actin during dormancy breaking suggests changes in cell functioning and seed preparation to germination. Secondary dormant seeds were characterized by low levels of legumin, metabolic enzymes, and actin, suggesting the consumption of storage materials, a decrease in metabolic activity, and cell elongation. Breaking the dormancy of rose seeds increased the abundance of cellular and metabolic proteins that promote germination. Induction of secondary dormancy caused a decrease in these proteins and germination arrest.

show abstract

“…Phytohormones play a key role in seed development processes such as maturation, dormancy and germination. The balance between abscisic acid (ABA) and gibberellic acid (GA) is considered to be the key to seed dormancy and germination, but not the absolute concentration or content of ABA and GA (Arc et al 2013, Deng et al 2016, Shu et al 2016. Generally, bioactive GA contributes to embryo development (Lulsdorf & Ying 2013), and ABA is the most prominent germination-inhibiting hormone in dormancy and germination control (Chen et al 2008, Bian et al 2018.…”

Section: Introductionmentioning

confidence: 99%

Physiological dormancy and dormancy release of Sassafras tzumu, a colored-leaf tree species with high landscape and economic value

Chen¹,

Jiang²,

Liu³

et al. 2022

iForest

View full text Add to dashboard Cite

Sassafras tzumu is one of the most valuable tree species in southern China. However, the dormancy of the seed limits its seedling quality. In order to improve the germination percentage of seeds, characteristics, causes of dormancy, changes in endogenous hormones and nutrients during storage were investigated. The results showed that seed viability was 78.11%, and the highest water absorption rate was 26.09%. The germination percentage of the embryo without cotyledon was 21.13%, but intact seed and embryo could not germinate on hormone-free medium. Seed embryo and coat extracts were found to have a significant inhibitory effect on seed germination. Germination percentage were significantly positively correlated to free amino content (p < 0.01) and negatively correlated to soluble sugar content (p < 0.05) during storage. The levels of GA3 and GA3/ABA had significant effects on the germination percentage of S. tzumu seed storage at 4°C. The results suggest that S. tzumu seeds are in intermediate physiological dormancy at maturity and are mainly caused by the presence of inhibitory substances in the seed tissues. Furthermore, changes in endogenous hormones and metabolism of nutrients at 4°C can significantly promote the release from dormancy of S. tzumu seeds.

show abstract

Dormancy release of Cotinus coggygria seeds under a pre-cold moist stratification: an endogenous abscisic acid/gibberellic acid and comparative proteomic analysis

Cited by 7 publications

References 42 publications

The coupled effect of light and temperature on dormancy release and germination of Pinus koraiensis seeds

The coupled effect of light and temperature on dormancy release and germination of Pinus koraiensis seeds

Temperature Regulation of Primary and Secondary Seed Dormancy in Rosa canina L.: Findings from Proteomic Analysis

Physiological dormancy and dormancy release of Sassafras tzumu, a colored-leaf tree species with high landscape and economic value

Contact Info

Product

Resources

About