Doratoxylon apetalum, an Indigenous Medicinal Plant from Mascarene Islands, Is a Potent Inhibitor of Zika and Dengue Virus Infection in Human Cells

Haddad, Juliano G.; Koishi, Andrea Cristine; Gaudry, Arnaud; Santos, Claudia Nunes Duarte dos; Viranaïcken, Wildriss; Desprès, Philippe; Kalamouni, Chaker El

doi:10.3390/ijms20102382

Cited by 26 publications

(34 citation statements)

References 48 publications

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“…The treatments were able to prevent infection of both strains of ZIKV in trophoblast cells (A, B and D). Experiments were performed in n = 6. from Mascarene Island, which inhibited Zika and Dengue virus infection in leukocytes (Haddad et al, 2019). One polyphenol present in large amounts in the green tea, (−)-epigallocatechin gallate (EGCG), has been also described as a potent antiviral molecule, able to inhibit ZIKV entry in Vero E6 cells (Carneiro et al, 2016).…”

Section: Flow Cytometry Analysis On Zikv Infection Of Htr-8/svneo Cellsmentioning

confidence: 99%

Schinus terebenthifolius Raddi extracts: From sunscreen activity toward protection of the placenta to Zika virus infection, new uses for a well-known medicinal plant

Oliveira

Valentim

Rocha

et al. 2020

Industrial Crops and Products

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Schinus terebinthifolius Raddi is a well-known medicinal plant native of South America. This species has demonstrated important biological activities such as antihypertensive and vasodilator, antimicrobial, anti-inflammatory and antioxidant. However, no studies have been, so far, reported with the fruits of S. terebinthifolius as a protector of the placenta against Zika virus infection and as sunscreen agents. The present study aimed to investigate new uses for the ethanolic fruit extracts of S. terebinthifolius, from fruits'peel (STPE) and from the whole fruits (STWFE). Zika virus (ZIKV) has been linked to several fetal malformations, such as microcephaly and other central nervous system abnormalities. Thus, the potential of these natural extracts against ZIKV infection was evaluated, using an in vitro method. The photoprotective potential, determined by spectrometry, along with phenolic content, antioxidant capacity, and chemical composition of both extracts were also evaluated. The chemical composition of the extracts was evaluated by HPLC-UV / vis. The cytotoxicity of peel and whole fruit extracts in vero E6 cell lines, in placental cell lines and placental explant cultures were evaluated by the MTT assay. The infectivity of placental cells and explants was evaluated by qRT-PCR and the effects of extracts on ZIKV infection were investigated using HTR-8/SVneo cells, pre-treated with 100 μg mL −1 of STWFE for 1 h, and infected with MR766 (AD) or PE243 (EH) ZIKV strains. STFE and STWFE were well-tolerated by both placental-derived trophoblast cell line HTR-8/SVneo as well as by term placental chorionic villi explants, which indicate absence of cytotoxicity in all analysed concentrations. Two strains of ZIKV were tested to access if pretreatment of trophoblast cells with the STWFE would protect them against infection. Flow cytometry analysis revealed that STWFE extract greatly reduced ZIKV infection. The extracts were also photoprotective with SPF values equivalent to the standard, benzophenone-3. The formulations prepared in different concentrations of the extracts (5-10 %) had shown maximum SPF values of 32.21. STWFE represents a potential natural mixture to be used in pregnancy in order to restrain placental infection by ZIKV and might potentially protect fetus against ZIKV-related malformations. The extracts exhibited photoprotective activity and some of the phenolic compounds, mainly resveratrol, catechin and epicatechin, are active ingredients in all assayed activities. The development of biotechnological/medical products, giving extra value to products from family farming, is expected, with strong prospects for success.

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Section: Flow Cytometry Analysis On Zikv Infection Of Htr-8/svneo Cellsmentioning

confidence: 99%

Schinus terebenthifolius Raddi extracts: From sunscreen activity toward protection of the placenta to Zika virus infection, new uses for a well-known medicinal plant

Oliveira

Valentim

Rocha

et al. 2020

Industrial Crops and Products

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“…The current search for ZIKV antiviral options is being conducted through several different approaches which target different steps of the replication cycle: by screening of different compound libraries or by the repurposing of drugs with known active efficacy against other diseases and that are already being used in clinic. In addition, natural products [40,41] as well as antibody-based candidates [42,43] are being evaluated but are outside the scope of this review. Antivirals can be classified according to their mode of action in (i) direct-acting antivirals, directed against viral targets, or (ii) host-targeting antivirals, aimed at targeting cellular components needed for the viral cycle.…”

Section: Potential Therapeutic Options For the Treatment Of Zikv Imentioning

confidence: 99%

Antiviral Agents in Development for Zika Virus Infections

Baz

Boivin

2019

Pharmaceuticals

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In 1947, Zika virus (ZIKV), a mosquito-borne flavivirus was identified in Uganda and subsequently spread to Asia and the Pacific regions. In 2015, it was introduced in Brazil causing an important social and sanitary alarm due to its increased virulence and rapid dissemination. Importantly, ZIKV infections have been associated with severe neurological complications such as Guillain–Barré syndrome and microcephaly in fetuses and newborns. Although enormous efforts were made by investigators in the development of effective countermeasures against ZIKV, there is still no approved specific antiviral drug for the treatment of ZIKV infections. Herein, we review several anti ZIKV candidates including drugs targeting both the virus (structural proteins and enzymes) and cellular elements.

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“…Many of the indigenous and endemic plants from Reunion island have been and are still used for traditional medicine [ 3 ]. Although some studies have reported the potential therapeutic effects of these plants in combatting hypertension [ 4 ], oxidative stress, inflammation [ 5 ], parasitosis (i.e., plasmodium), and viruses (i.e., Chikungunya, Dengue, and Zika) [ 6 , 7 , 8 ], their deep content characterization as well as their real efficiency in vivo remains largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Phenolic Profile of Herbal Infusion and Polyphenol-Rich Extract from Leaves of the Medicinal Plant Antirhea borbonica: Toxicity Assay Determination in Zebrafish Embryos and Larvae

Veeren¹,

Ghaddar²,

Bringart³

et al. 2020

Molecules

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Antirhea borbonica (A. borbonica) is an endemic plant from the Mascarene archipelago in the Indian Ocean commonly used in traditional medicine for its health benefits. This study aims (1) at exploring polyphenols profiles from two types of extracts—aqueous (herbal infusion) and acetonic (polyphenol rich) extracts from A. borbonica leaves—and (2) at evaluating their potential toxicity in vivo for the first time. We first demonstrated that, whatever type of extraction is used, both extracts displayed significant antioxidant properties and acid phenolic and flavonoid contents. By using selective liquid chromatography–tandem mass spectrometry, we performed polyphenol identification and quantification. Among the 19 identified polyphenols, we reported that the main ones were caffeic acid derivatives and quercetin-3-O-rutinoside. Then, we performed a Fish Embryo Acute Toxicity test to assess the toxicity of both extracts following the Organisation for Economic Cooperation and Development (OECD) guidelines. In both zebrafish embryos and larvae, the polyphenols-rich extract obtained by acetonic extraction followed by evaporation and resuspension in water exhibits a higher toxic effect with a median lethal concentration (LC50: 5.6 g/L) compared to the aqueous extract (LC50: 20.3 g/L). Our data also reveal that at non-lethal concentrations of 2.3 and 7.2 g/L for the polyphenol-rich extract and herbal infusion, respectively, morphological malformations such as spinal curvature, pericardial edema, and developmental delay may occur. In conclusion, our study strongly suggests that the evaluation of the toxicity of medicinal plants should be systematically carried out and considered when studying therapeutic effects on living organisms.

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Doratoxylon apetalum, an Indigenous Medicinal Plant from Mascarene Islands, Is a Potent Inhibitor of Zika and Dengue Virus Infection in Human Cells

Cited by 26 publications

References 48 publications

Schinus terebenthifolius Raddi extracts: From sunscreen activity toward protection of the placenta to Zika virus infection, new uses for a well-known medicinal plant

Schinus terebenthifolius Raddi extracts: From sunscreen activity toward protection of the placenta to Zika virus infection, new uses for a well-known medicinal plant

Antiviral Agents in Development for Zika Virus Infections

Phenolic Profile of Herbal Infusion and Polyphenol-Rich Extract from Leaves of the Medicinal Plant Antirhea borbonica: Toxicity Assay Determination in Zebrafish Embryos and Larvae

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