Doppler and oscillometric mean blood pressure best represent direct blood pressure measurements in anesthetized rhesus macaques (<i>Macaca mulatta</i>)

Kang, Stacey; Jampachaisri, Katechan; Pacharinsak, Cholawat

doi:10.1111/jmp.12397

Cited by 7 publications

(7 citation statements)

References 16 publications

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“…These MAP measurements were lower than expected. According to recent literature, oscillometric monitoring of BP is often underestimated ( 29 , 30 ). None of the monkeys in the experiment died because of the experimental treatment.…”

Section: Resultsmentioning

confidence: 99%

Recombinant Fasciola hepatica Fatty Acid Binding Protein as a Novel Anti-Inflammatory Biotherapeutic Drug in an Acute Gram-Negative Nonhuman Primate Sepsis Model

et al. 2021

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Section: Resultsmentioning

confidence: 99%

Recombinant Fasciola hepatica Fatty Acid Binding Protein as a Novel Anti-Inflammatory Biotherapeutic Drug in an Acute Gram-Negative Nonhuman Primate Sepsis Model

et al. 2021

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“…These MAP measurements were lower than expected. According to recent literature oscillometric monitoring of BP are often underestimated (26, 27). At the conclusion of the procedure (8h) the physiological parameters were similar or slightly higher than the baseline for most of animals, which were euthanized without signs of septic shock…”

Section: Resultsmentioning

confidence: 99%

Recombinant Fasciola hepatica fatty acid binding protein (Fh15) as a novel anti-inflammatory biotherapeutic in an acute gram-negative non-human primate sepsis model

Rosado‐Franco

Armina-Rodríguez

Marzan-Rivera

et al. 2021

Preprint

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Due to their phylogenetic proximity to human, non-human primates (NHP) are considered and adequate choice for basic and pre-clinical model of sepsis. Gram-negative bacteria are the primary causative of sepsis. During infection bacteria continuously release the potent toxin lipopolysaccharide (LPS) into the bloodstream, which triggers an uncontrolled systemic inflammatory response leading to death. Our previous research has demonstrated in vitro and in vivo using a mouse model of septic shock that Fh15, a recombinant variant of Fasciola hepatica fatty acid binding protein, acts as an antagonist of TLR4 suppressing the LPS-induced pro-inflammatory storm. The present study aimed to demonstrate that Fh15 suppress the cytokine storm and other inflammatory markers during the early phase of endotoxemia induced in rhesus macaques by i.v. infusion with lethal doses of live E. coli. Fh15 was administered as isotonic infusion 30 min prior to the bacterial infusion. Among the novel findings reported in this communication, I) Fh15 significantly prevents bacteremia, suppressed LPS levels in plasma and the production of C-reactive protein and procalcitonin, which are key signature of inflammation and bacterial infection, respectively, II) notably reduced the production of pro-inflammatory cytokines, and III) increased innate immune cell populations in blood, which suggest a role in promoting a prolonged steady state in rhesus macaques even in the presence of inflammatory stimuli. THis is the first report demonstrating that a F. hepatica-derived molecule possesses potential as anti-inflammatory drug against endotoxemia in an NHP model.

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“…The average of 3 measures was used to identify animals with a mean blood pressure greater than 110 mm Hg or systolic blood pressure greater than 160 mmHg; these macaques were excluded from the study to eliminate phenocopies of HCM. 1,17 Due to the sequence of events, the blood pressure measurement was always and only assessed under ketamine sedation.…”

Section: Ethics Statement the California National Primate Researchmentioning

confidence: 99%

Vertebral Heart Score in Rhesus Macaques (Macaca mulatta): Generating Normal Reference Intervals and Assessing its Validity for Identifying Cardiac Disease

Williams¹,

Ueda²,

Stern³

et al. 2020

j am assoc lab anim sci

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Vertebral heart scoring (VHS) is a semiquantitative method to assess the presence and severity of cardiomegaly by usingthoracic radiographs. VHS in rhesus macaques (Macaca mulatta) has not been validated or used routinely in the clinical orresearch setting. We hypothesized that rhesus macaques with cardiac disease diagnosed by using echocardiography wouldhave higher VHS than animals without cardiac disease. A total of 150 rhesus macaques were enrolled in this study. All animalsunderwent echocardiography and thoracic radiography (right lateral [RL], dorsoventral [DV], and ventrodorsal [VD] views).According to echocardiography, 121 rhesus macaques had no cardiac disease and were used to establish reference intervalsfor VHS. The remaining 29 macaques had hypertrophic cardiomyopathy (n = 20) or other cardiac disease (n = 9). Resultsshowed that VHS of RL and VD views were significantly higher in macaques with any of the identified cardiac diseases andin the cardiac disease group that excluded hypertrophic cardiomyopathy. VHS of animals with HCM was not significantlydifferent than that of control animals. In the RL view, VHS was moderately accurate for predicting the presence of cardiacdisease, with an AUC of 0.71 and an optimal cut-off value of 10.25 (sensitivity: 62%, specificity: 77%). In the VD view, VHSwas a mildly accurate test for cardiac disease, with an AUC of 0.654 and an optimal cut-off value of 10.65 (sensitivity, 66%;specificity, 63%). Study results indicated that VHS could be a useful screening tool for clinically identifying rhesus macaqueswith cardiac disease. However, VHS is unlikely to replace echocardiographic examination for determining the presence, type,and severity of cardiac disease in this species.

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Doppler and oscillometric mean blood pressure best represent direct blood pressure measurements in anesthetized rhesus macaques (Macaca mulatta)

Cited by 7 publications

References 16 publications

Recombinant Fasciola hepatica Fatty Acid Binding Protein as a Novel Anti-Inflammatory Biotherapeutic Drug in an Acute Gram-Negative Nonhuman Primate Sepsis Model

Recombinant Fasciola hepatica Fatty Acid Binding Protein as a Novel Anti-Inflammatory Biotherapeutic Drug in an Acute Gram-Negative Nonhuman Primate Sepsis Model

Recombinant Fasciola hepatica fatty acid binding protein (Fh15) as a novel anti-inflammatory biotherapeutic in an acute gram-negative non-human primate sepsis model

Vertebral Heart Score in Rhesus Macaques (Macaca mulatta): Generating Normal Reference Intervals and Assessing its Validity for Identifying Cardiac Disease

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