Doping control analysis in human urine by liquid chromatography–electrospray ionization ion trap mass spectrometry for the Olympic Games Athens 2004: Determination of corticosteroids and quantification of ephedrines, salbutamol and morphine

Spyridaki, M.-H.; Kiousi, Polyxeni; Vonaparti, Ariadni; Valavani, Paraskevi; Zonaras, Vassilios; Zahariou, M.; Sianos, Efstathios; Tsoupras, G.; Georgakopoulos, Costas

doi:10.1016/j.aca.2006.04.042

Cited by 67 publications

(43 citation statements)

References 11 publications

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“…LC-MS screening methods using linear gradients for a complex mixture of CC and anabolic STER in horse urine [8] and for detecting ten anabolic STER in muscle tissues [9] have been developed. Another LC-MS method using a segmented gradient for the determination of CC in human urine has also been developed [10]. An LC-TOF-MS method using a segmented gradient for a group of CC in human and animal urine has also been i …”

Section: Introductionmentioning

confidence: 99%

Quantitative screening for steroids in animal feeding water using reversed phase LC with gradient elution

Muñiz‐Valencia¹,

Gonzalo-Lumbreras²,

Santos-Montes³

et al. 2008

J of Separation Science

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Several isocratic separations for the determination of 20 steroids (STER) in animal feeding water samples (AFWS) from drinking-trough by LC using a mobile phase ACN/H(2)O (35:65 v/v) and different RP columns (Hypersil C18, Gemini C18 (GM), Purospher Star C18, Synergi Max C12, and Synergi Fusion) and UV detection were obtained. The elution order was the same: a first group of corticoids (CC) was early eluted, a second group of CC and anabolics (AAS) exhibited intermediate retention, and a third group constituted by AAS was strongly retained. To improve the separation performances of the isocratic separations an ACN gradient elution optimization was carried out for each column. The most satisfactory results were obtained using a Purospher column which allowed the separation of 19 STER in an analysis time close to 26 min. After sample preparation using SPE, method validation was performed in an AFWS spiked with STER according to the EC decision criteria established for quantitative screening methods. For this purpose calibration graphs, extraction efficiencies, decision limits, detection capabilities, precision (repeatability and within-laboratory reproducibility), accuracy, selectivity, and robustness were evaluated. The proposed method was applied to other AFWS with satisfactory results.

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Section: Introductionmentioning

confidence: 99%

Quantitative screening for steroids in animal feeding water using reversed phase LC with gradient elution

Muñiz‐Valencia¹,

Gonzalo-Lumbreras²,

Santos-Montes³

et al. 2008

J of Separation Science

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“…[1][2][3][4] At present, routine testing techniques in athletic sports are centred around multidimensional instrumental approaches for abused-drug analysis, such as gas chromatography-mass spectrometry (GC-MS), [5] highperformance liquid chromatography (HPLC), [6,7] capillary electrophoresis (CE), [8] and matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF-MS), [9] and may incorporate tandem mass spectrometry (MS-MS) [10] approaches for enhanced sensitivity and/or identification. These current approaches clearly involve not only expensive instruments, but also a large number of separate analytical procedures, resulting in a more-complex, timeconsuming and laborious screening procedure.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive Specific Stimulant Assay Based on Molecularly Imprinted Photonic Hydrogels

et al. 2008

Adv Funct Materials

124

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Taking theophylline and (1R,2S)‐(−)‐ephedrine as template molecules, two imprinted photonic‐hydrogel films are prepared by a combination of colloidal‐crystal and molecular‐imprinting techniques. This paper shows a new approach for rapid and handy stimulant detection with high sensitivity and specificity. One film is proposed for analogous molecule assay, another one for chiral recognition. The key point of this approach is that the imprinted photonic polymer (IPP) consists of a three‐dimensional (3D), highly‐ordered and interconnected macroporous array with a thin hydrogel wall, where nanocavities complementary to analytes in shape and binding sites are distributed. This special, bicontinuous, hierarchical structure enables this polymer to report quickly, easily, sensitively and directly a molecular recognition event without any transducers and treatments for analytes (label‐free). The inherent affinity of the nanocavities, deriving from molecular imprinting, makes these sensors highly specific to analytes, even if in a competitive environment. Their sensitive and specific responses to stimulants in buffer are determined by Bragg diffractive shifts due to the lattice change of their 3D ordered macroporous arrays resulting from their preferential rebinding to the target molecules. The measurements show that the prepared hydrogel films exhibit high sensitivity in such a 0.1 fM concentration of analytes and specificity even in a competitive urinous buffer. The reported method provides a rapid and handy approach for stimulant assay and drug analysis in athletic sports.

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“…For safety precautions, sensitive monitoring of MO in blood or urine is necessary. Several methods have been developed for the detection and determination of MO, including sequential injection analysis method [13], cation-selective exhaustive injection and sweeping micellar electrokinetic chromatography [14], fast Fourier transformation with continuous cyclic voltammetry method [15], gas chromatography -mass spectrometry [16], high-performance liquid chromatography -fluorescence method [17], liquid chromatography -electrospray ionization ion trap mass spectrometry [18], and microchip method [19]. However, these analytical methods are time-consuming and devices are expensive.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Oxidation and Detection of Morphine at Ordered Mesoporous Carbon Modified Glassy Carbon Electrodes

2009

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In this work, three ordered mesoporous carbons (OMCs) with different structural parameters were synthesized by a simple variation of the hydrothermal temperature of the silica templates (SBA-15). X-ray diffraction and nitrogen adsorption-desorption results show these OMCs exhibit an ordered 2D hexagonal mesostructure with tunable pore diameter. OMC-modified glassy carbon electrodes exhibit efficient electrocatalytic reactivity toward oxidation of morphine (MO). The amperometric detection of MO in pH 7.0 phosphate buffered saline at þ 0.39 V versus Ag/AgCl is the lowest potential reported to-date. A linear range from 0.2 to 197.6 mM and a detection limit of 0.03 mM MO were obtained.

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Doping control analysis in human urine by liquid chromatography–electrospray ionization ion trap mass spectrometry for the Olympic Games Athens 2004: Determination of corticosteroids and quantification of ephedrines, salbutamol and morphine

Cited by 67 publications

References 11 publications

Quantitative screening for steroids in animal feeding water using reversed phase LC with gradient elution

Quantitative screening for steroids in animal feeding water using reversed phase LC with gradient elution

Ultrasensitive Specific Stimulant Assay Based on Molecularly Imprinted Photonic Hydrogels

Electrochemical Oxidation and Detection of Morphine at Ordered Mesoporous Carbon Modified Glassy Carbon Electrodes

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