Dopaminergic transmission in the rat retina: evidence for volume transmission

Bjelke, Börje; Goldstein, Menek; Tinner, B.; Andersson, Cecilia; Sesack, Susan R.; Steinbusch, Harry W.M.; Lew, J.; He, Xi; Watson, S.J.; Tengroth, Björn; Fuxé, Kjell

doi:10.1016/s0891-0618(96)00176-7

Cited by 69 publications

(58 citation statements)

References 57 publications

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“…The amacrine and/or the inner plexiform DA nerve cells are located in the inner nuclear layer, with their terminal branches mainly innervating the inner plexiform layer and with very few radial branches into the outer plexiform layer which contain a high density of dopamine D1 and D2 receptors. Mismatch DA receptors are also found within the outer segment of the photoreceptors and in the ganglionic cell layer (Djamgoz and Wagner, 1992;Witkovsky et al, 1993;Bjelke et al, 1996). These results had inter alia been obtained by DA D1 and D2 receptor immunocytochemistry in combination with tyrosine hydroxylase immunocytochemistry and by dopamine D1 and D2 receptor autoradiography.…”

Section: Location Of Transmitter Receptors Outside the Postsynaptic Dmentioning

confidence: 56%

From the Golgi–Cajal mapping to the transmitter-based characterization of the neuronal networks leading to two modes of brain communication: Wiring and volume transmission

Fuxé¹,

Dahlström²,

Höistad³

et al. 2007

Brain Research Reviews

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209

219

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After Golgi-Cajal mapped neural circuits, the discovery and mapping of the central monoamine neurons opened up for a new understanding of interneuronal communication by indicating that another form of communication exists. For instance, it was found that dopamine may be released as a prolactin inhibitory factor from the median eminence, indicating an alternative mode of dopamine communication in the brain. Subsequently, the analysis of the locus coeruleus noradrenaline neurons demonstrated a novel type of lower brainstem neuron that monosynaptically and globally innervated the entire CNS.Furthermore, the ascending raphe serotonin neuron systems were found to globally innervate the forebrain with few synapses, and where deficits in serotonergic function appeared to play a major role in depression. We propose that serotonin reuptake This will lead to the unified execution of information handling and trophism for optimal brain function and survival.

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Section: Location Of Transmitter Receptors Outside the Postsynaptic Dmentioning

confidence: 56%

From the Golgi–Cajal mapping to the transmitter-based characterization of the neuronal networks leading to two modes of brain communication: Wiring and volume transmission

Fuxé¹,

Dahlström²,

Höistad³

et al. 2007

Brain Research Reviews

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209

219

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“…Adult rat retinas were used for all of the experiments reported here for a number of reasons: the presence, synthesis, and release of dopamine, as well as dopamine receptor activation and regulation of cAMP, have been demonstrated; the anatomy and distribution of dopaminesensitive cell types are known; and depletion of dopamine has a conspicuous behavioral effect (Malmfors, 1963;Pycock and Smith, 1983;Voigt and Wä ssle, 1987;Bjelke et al, 1996;Shulman and Fox, 1996;Feigenspan et al, 2001;Puopolo et al, 2001;Patel et al, 2003). Sprague-Dawley rats (female, P60 -P120, 150 -250 g) were obtained from a commercial supplier (Harlan Bioproducts; Indianapolis, IN) and housed in standard cages at room temperature (ϳ23°C) on a 12-hour/12-hour light/ dark cycle.…”

Section: Materials and Methods Animalsmentioning

confidence: 99%

DARPP‐32‐like immunoreactivity in AII amacrine cells of rat retina

Partida

Lee

Haft-Candell

et al. 2004

J of Comparative Neurology

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Previous studies demonstrated that the dopamine- and adenosine 3',5'-monophosphate-regulated phosphatase inhibitor known as "DARPP-32" is present in rat, cat, monkey, and human retinas. We have followed up these studies by asking what specific cell subtypes contain DARPP-32. Using a polyclonal antibody directed against a peptide sequence of human DARPP-32, we immunostained adult rat retinas that were either transretinally sectioned or flat mounted and found DARPP-32-like immunoreactivity in some cells of the amacrine cell layer across the entire retinal surface. We report here, based on the shape and spatial distribution of these cells, their staining by an anti-parvalbumin antibody, and their juxtaposition with processes containing tyrosine hydroxylase, that DARPP-32-like immunoreactivity is present in AII amacrine cells of rat retina. These results suggest that the response of AII amacrine cells to dopamine is not mediated as simply as previously supposed.

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“…Cells were washed with PBS containing 20 mM glycine and subsequently exposed to PBS/20 mM glycine/1% BSA for 30 min at room temperature. Double immunostaining was performed with the affinity-purified rabbit anti-D 2 antibody (Bjelke et al 1996) (1:800) and mouse anti-HA antibody (Roche SpA; Milano, Italy) (1:100) in PBS, pH 7.4, supplemented with 1% goat serum at 4C overnight.…”

Section: Double-immunolabeling Experiments For Immunofluores-mentioning

confidence: 99%

New Methods to Evaluate Colocalization of Fluorophores in Immunocytochemical Preparations as Exemplified by a Study on A_2Aand D₂Receptors in Chinese Hamster Ovary Cells

Agnati¹,

Fuxé

Torvinen

et al. 2005

J Histochem Cytochem.

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S U M M A R YAn important aspect of the image analysis of immunocytochemical preparations is the evaluation of colocalization of different molecules. The aim of the present study is to introduce image analysis methods to identify double-labeled locations exhibiting the highest association of two fluorophores and to characterize their pattern of distribution. These methods will be applied to the analysis of the cotrafficking of adenosine A 2A and dopamine D 2 receptors belonging to the G protein-coupled receptor family and visualized by means of fluorescence immunocytochemistry in Chinese hamster ovary cells after agonist treatment. The present procedures for colocalization have the great advantage that they are, to a large extent, insensitive to the need for a balanced staining with the two fluorophores. Thus, these procedures involve image processing, visualization, and analysis of colocalized events, using a covariance method and a multiply method and the evaluation of the identified colocalization patterns. Moreover, the covariance method offers the possibility of detecting and quantitatively characterizing anticorrelated patterns of intensities, whereas the immediate detection of colocalized clusters with a high concentration of labeling is a possibility offered by the multiply method. The present methods offer a new and sensitive approach to detecting and quantitatively characterizing strongly associated fluorescence events, such as those generated by receptor-receptor interaction, and their distribution patterns in dual-color confocal laser microscopy.

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Dopaminergic transmission in the rat retina: evidence for volume transmission

Cited by 69 publications

References 57 publications

From the Golgi–Cajal mapping to the transmitter-based characterization of the neuronal networks leading to two modes of brain communication: Wiring and volume transmission

From the Golgi–Cajal mapping to the transmitter-based characterization of the neuronal networks leading to two modes of brain communication: Wiring and volume transmission

DARPP‐32‐like immunoreactivity in AII amacrine cells of rat retina

New Methods to Evaluate Colocalization of Fluorophores in Immunocytochemical Preparations as Exemplified by a Study on A_2Aand D₂Receptors in Chinese Hamster Ovary Cells

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Dopaminergic transmission in the rat retina: evidence for volume transmission

Cited by 69 publications

References 57 publications

From the Golgi–Cajal mapping to the transmitter-based characterization of the neuronal networks leading to two modes of brain communication: Wiring and volume transmission

From the Golgi–Cajal mapping to the transmitter-based characterization of the neuronal networks leading to two modes of brain communication: Wiring and volume transmission

DARPP‐32‐like immunoreactivity in AII amacrine cells of rat retina

New Methods to Evaluate Colocalization of Fluorophores in Immunocytochemical Preparations as Exemplified by a Study on A2Aand D2Receptors in Chinese Hamster Ovary Cells

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New Methods to Evaluate Colocalization of Fluorophores in Immunocytochemical Preparations as Exemplified by a Study on A_2Aand D₂Receptors in Chinese Hamster Ovary Cells