Dopaminergic nerve endings visualised by high-resolution autoradiography in adult rat neostriatum

Descarries, Laurent; Bosler, Olivier; Berthelet, France; Rosiers, M.H. Des

doi:10.1038/284620a0

Cited by 79 publications

(18 citation statements)

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“…After fixation of the brain by glutaraldehyde and osmium tetroxide fixatives perfused in rapid succession, small and dense accumulations of silver grains typical of monoaminelabeled terminals are then detected in the paraventricular neostriatum not only following the in vivo administration of 13H]DA (Descarries et al, 1980(Descarries et al, ,1981 or of ["Hlnoradrenaline (unpublished) but even after administration of [3H]adrenaline (Bosler and Descarries, 1983). The DA selectivity of these tritiated catecholamine accumulations Figure 13, these varicosities (Figs.…”

Section: Methodological Considerationsmentioning

confidence: 93%

“…Similar to earlier investigations of rat brain tissue that was prepared for autoradiography after [3HlDA-uptake in vivo (Descarries et al, 1980(Descarries et al, , 1981; see also Descarries and Figs. 1-3.…”

Section: Autoradiographic Visualizationmentioning

confidence: 95%

“…Their average short axis (0.45-0.50 km), which was markedly greater than the transverse diameter of parent axons (0.24 Lm), confirmed the rarity of axonal segments filled with synaptic vesicles, perhaps due to the preferential labeling or immunostaining of varicosities. The average diameter (0.6-0.7 Fm) of [3HlDA-labeled or DA-immunostained varicosity profiles was in keeping with previous measurements in the neostriatum of rat or monkey (Descarries et al, 1980(Descarries et al, , 1981Pickel et al, 1981, 199213;Bosler and Descarries, 1983;Arluison et al, 1984;Kubota et al, 1986Kubota et al, , 1987aZahm, 1992;Smith et al, 1994). These data also indicated that the DA varicosities in neostriatum were only slightly larger than those containing serotonin (Soghomonian et al, 1989) but much larger than those containing acetylcholine (choline acetyltransferase immunostained; Contant et al, 1996;see also DiFiglia, 1987).…”

Section: Intrinsic Featuresmentioning

confidence: 96%

“…The autoradiographic material of the present study was from a single rat (Sprague Dawley male, body weight 250 g) and was prepared in conditions that had been demonstrated previously to ensure specific labeling of DA terminals after intraventricular administration of tritiated DA ([3H]DA) in vivo (Descarries et al, 1980(Descarries et al, , 1981 for a further description, see Descarries and Beaudet, 1983). Following pretreatment with a monoamine oxidase inhibitor (pargyline hydrochloride, 75 mgikg i.p.…”

Section: L3h1da Autoradiographymentioning

confidence: 99%

“…A few years later, the initial description by Pickel et al (1976) of neostriatal terminals immunostained with specific antibodies directed against tyrosine hydroxylase (TH) seemed to confirm this finding in aldehyde-fixed tissue. In 1980, a preliminary report from our laboratory indicated that, in adult rat neostriatum that was autoradiographed after uptake labeling with r3H]DA in vivo, a considerable number of sectional profiles from DA terminals exhibited small, symmetrical synaptic membrane specializations with dendritic branches or spines, raising the possibility of a relatively high synaptic frequency (Descarries et al, 1980; also see Descarries et al, 1981). Independently, a further TH-immunocytochemical study from Pickel's laboratory (1981) that was also carried out in single thin sections suggested the existence of two categories of neostriatal DA terminals: a restricted one with larger varicosities that were deprived of synaptic membrane specializations and a more abundant one with smaller varicosities exhibiting specializations that were indeed symmetrical and were found occasionally on neuronal somata as well as on dendritic branches and spines (Pickel et al, 1981; see also Kaiya and Namba, 1981;Arluison et al, 1984;Triarhou et al, 1988a).…”

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confidence: 94%

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Dual character, asynaptic and synaptic, of the dopamine innervation in adult rat neostriatum: A quantitative autoradiographic and immunocytochemical analysis

et al. 1996

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Dopamine (DA) axon terminals (varicosities) in the neostriatum of adult rats were examined for shape, size, content, synaptic incidence, type of junction, synaptic targets, and microenvironment after electron microscopic identification either by [3H]DA uptake autoradiography or by immunocytochemistry with monoclonal antibodies against DA-glutaraldehyde-protein conjugate. Both approaches yielded comparable results. Whether they were from the paraventricular or the mediodorsal neostriatum, respectively, the [3H]DA-labeled and DA-immunostained varicosities were generally oblong and relatively small; more than 60% contained one or more mitochondria. Sixty to seventy percent were asynaptic, and 30-40% were endowed with a synaptic membrane differentiation (junctional complex), as inferred by stereological extrapolation from single thin sections (both approaches) or observed directly in long, uninterrupted series of thin sections (immunocytochemistry). The synaptic DA varicosities always displayed symmetrical junctions: 67% with dendritic branches, 30% with dendritic spines, and 2-3% with neuronal cell bodies. DA varicosities juxtaposed to one another were frequent. Other axonal varicosities were more numerous in the immediate vicinity of DA varicosities than around randomly selected, unlabeled terminals. The respective microenvironments of DA and unlabeled varicosities also showed enrichment in the preferred synaptic targets of both groups of varicosities, with dendritic branches for DA and dendritic spines for the unlabeled ones. These data suggest a dual mode of operation that is diffuse as well as synaptic for the nigrostriatal DA system. In such a densely DA-innervated brain region, they also lead to the hypothesis that a basal level of extracellular DA might be maintained permanently around every tissue constituent and, thus, contribute to the mechanisms of action, properties, and functions (or dysfunctions) of DA within the neostriatum itself and as part of the basal ganglia circuitry.

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Section: Methodological Considerationsmentioning

confidence: 93%

Section: Autoradiographic Visualizationmentioning

confidence: 95%

Section: Intrinsic Featuresmentioning

confidence: 96%

Section: L3h1da Autoradiographymentioning

confidence: 99%

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confidence: 94%

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Dual character, asynaptic and synaptic, of the dopamine innervation in adult rat neostriatum: A quantitative autoradiographic and immunocytochemical analysis

et al. 1996

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Subcellular localization and molecular topology of the dopamine transporter in the striatum and substantia nigra

et al. 1997

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Plasma membrane transporters remove neurotransmitters from the extracellular space and have been postulated to terminate synaptic activity. Their specific roles in synaptic and nonsynaptic neurotransmission at a cellular level, however, remain unclear. We have determined the subcellular location of the dopamine transporter (DAT) by immunoperoxidase and immunogold electron microscopy, using monoclonal antibodies to both the N-terminus and the second extracellular loop. The two DAT epitopes were found on opposite faces of cellular and intracellular membranes, providing confirmation of the predicted molecular topology of DAT. In the striatum, DAT was localized in the plasma membrane of axons and terminals. Double immunocytochemistry demonstrated DAT colocalization with two other markers of nigrostriatal terminals, tyrosine hydroxylase and D2 dopamine receptors. The latter was thus demonstrated to be an autoreceptor. Labeled striatal terminals formed symmetrical synapses with spines, dendrites, and perikarya. DAT was not identified within any synaptic active zones, however, even using serial section analysis. These results suggest that striatal dopamine reuptake may occur outside of synaptic specializations once dopamine diffuses from the synaptic cleft. In the substantia nigra, DAT appears to be specifically transported into dendrites, where it can be found in smooth endoplasmic reticulum, plasma membrane, and pre- and postsynaptic active zones. These localizations suggest that DAT modulates the intracellular and extracellular dopamine levels of nigral dendrites. Within the perikarya of pars compacta neurons, DAT was localized primarily to rough and smooth endoplasmic reticulum, Golgi complex, and multivesicular bodies, identifying probable sites of synthesis, modification, transport, and degradation.

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γ‐Aminobutyric Acid and Basal Ganglia Outflow Pathways

Reavill

Jenner

Marsden

1984

Ciba Foundation Symposium 107 ‐ Functions of the Basal Ganglia

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Dopaminergic nerve endings visualised by high-resolution autoradiography in adult rat neostriatum

Cited by 79 publications

References 27 publications

Dual character, asynaptic and synaptic, of the dopamine innervation in adult rat neostriatum: A quantitative autoradiographic and immunocytochemical analysis

Dual character, asynaptic and synaptic, of the dopamine innervation in adult rat neostriatum: A quantitative autoradiographic and immunocytochemical analysis

Subcellular localization and molecular topology of the dopamine transporter in the striatum and substantia nigra

γ‐Aminobutyric Acid and Basal Ganglia Outflow Pathways

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