Dopamine uptake sites in the striatum are distributed differentially in striosome and matrix compartments.

Abstract

A major mechanism of neurotransmitter inactivation at catecholaminergic synapses is reuptake ofreleased transmitter at high-affinity uptake sites on presynaptic terminals. We have analyzed the anatomical distribution of siteselective ligand binding for dopamine uptake sites in the striatum of rat, cat, and monkey. We report here that desipramine-sensitive [31H]mazindol binding sites have highly heterogeneous distributions in the dorsal and the ventral striatum.

Differential vulnerability of primate caudate-putamen and striosome-matrix dopamine systems to the neurotoxic effects of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.

“…2). Second, the most dramatic instances of striosomal preservation of binding were in the putamen and lateral caudate nucleus of the MPTP-treated monkeys, particularly caudally, whereas the relatively re- (24,26). In fact, in one of our control monkeys (SqlO), dorsolateral striosomes even appeared as regions of slightly (4-10%) elevated binding (Fig.…”

“…For example, it has been proposed that differences in monoamine uptake-site concentration in different regions of the normal striatum could result in lower rates of uptake of the toxic metabolites of MPTP or MPTP-like substances in some regions (such as ventral striatum) than in others and, consequently, lower relative rates of nigrostriatal terminal damage (24,30). Our findings confirm relatively low levels of uptakesite binding in the ventral striatum of normal monkeys and show a relative sparing of uptake-site binding in the ventral striatum of MPTP-treated monkeys.…”

“…Binding incubations were performed as described previously (19,24) on two complete sets of sections for each brain and on additional control and trial sections. Frozen sections were brought to room temperature, were preincubated for 5 min at 40C in 50 mM Tris-HCl buffer, pH 7.9, containing 120 mM NaCl and 5 mM KCI to wash off endogenous ligand, and were incubated for 40 min with 15 nM [3H]mazindol (22.7 Ci/mmol; Du Pont NEN; 1 Ci = 37 GBq) in 50 mM Tris HCl buffer containing 300 mM NaCl, 5 mM Abbreviation: MPTP, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine.…”

Compartmentalization of excitatory amino acid receptors in human striatum.

“…Striatal projection neurons contain y-aminobutyric acid as a neurotransmitter but may be differentiated by the presence or absence of neuropeptides such as substance P, dynorphin, and enkephalin (33,36,37 (42) or predominantly in striosomes (19,43), and in human, cholinergic Ml binding sites (25) have been reported to be increased in striosomes relative to matrix. D2 binding sites in rat (15), dopamine uptake sites in rat, cat, primate, and human (14,23,26), cholinergic uptake sites in human (25), and benzodiazepine receptors in human (44) are increased in matrix. The major inputs to the mammalian neostriatum are glutamatergic (or aspartergic) corticostriatal afferents (11,45).…”

“…Recent evidence suggests that the neostriatum is able to maintain segregated information flow due to compartmentalization in mammalian caudate and putamen (1-11). Distinct regions within caudate and putamen have been defined in several species with histochemical techniques, in situ hybridization, and neurotransmitter receptor binding studies in rat (1,(12)(13)(14)(15), cat (10, 14, 16-20), primate (12, 14, 19-22), and human (20,(23)(24)(25)(26). These compartments, termed striosomes and matrix, exhibit distinct afferent and efferent connections.…”