Dopamine Signaling Regulates Fat Content through β-Oxidation in Caenorhabditis elegans

Barros, Alexandre Guimarães de Almeida; Bridi, Jéssika Cristina; Souza, Bruno R.; Júnior, Célio de Castro; Torres, Karen; Malard, Leandro M.; Jório, Ado; Miranda, Débora Marques de; Ashrafi, Kaveh; Romano-Silva, Marco Aurélio

doi:10.1371/journal.pone.0085874

Cited by 22 publications

(19 citation statements)

References 55 publications

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“…Since pathways involved in lipids catabolism are unknown in N. granulata (and to our knowledge in any other crab), further research is needed to test this hypothesis. Our results support the recently found reducing effects of DA on fat reserves in the nematode Caenorhabditis elegans (Maupas, 1900) revealing an ancient role for dopaminergic regulation of fat (Barros et al, 2014). Dopamine induces fat mobilization in some mammals (Thompson, 1984).…”

Section: Discussionsupporting

confidence: 91%

Lipase activity sensitive to dopamine, glucagon and cyclic AMP in the hepatopancreas of the euryhaline burrowing crab Neohelice granulata (Dana, 1851) (Decapoda, Grapsidae)

Michiels

Valle

Mañanes

2015

Crustac

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We studied the biochemical characteristics and modulation by dopamine, glucagon and cAMP of lipase activity in hepatopancreas of the euryhaline crab Neohelice granulata (Dana, 1851), considered to be an emergent model for biochemical, physiological and ecological research. Lipase activity was maximum at pH 8.5; it exhibited Michaelis-Menten kinetics (apparent K m = 0.018 mM), was highest at 37°C but appeared to be cold-and heat-tolerant, since it was high at 4°C and at 45°C. Lipase activity was enhanced upon incubation of hepatopancreas with 10~4 M dopamine (about 100%), 2 x 10~3 M glucagon (about 250%) and 10-4 M cAMP (about 150%) suggesting a role of these chemical messengers in mechanisms of regulation of lipolytic activities and its direct effect on the hepatopancreas. The concomitant decrease in triglycerides content upon dopamine and cAMP treatment suggests a link between enhanced lipase activity by these messengers and triglycerides catabolism. RESUMENEl objetivo de este trabajo fue estudiar caracterfsticas bioqui'micas y la modulation por dopamina, glucagon y AMPc in vitro de la actividad de lipasa en hepatopancreas del cangrejo eurihalino Neohelice granulata (Dana, 1851) el cual es considerado un modelo emergente para la realization de estudios sobre bioqulmica, fisiologfa y ecologi'a. La actividad de lipasa fue maxima a pH 8.5, exhibio una cinética de Michaelis-Menten (7Cm = 0.018 mM), el valor mas alto de actividad fue registrado a 37°C y es aparentemente tolerante al frio y al calor ya que mantuvo alta actividad a 4°C y 45°C. La actividad de lipasa en hepatopancreas se incrementô en presencia de dopamina 10" 4 M (100%), glucagon 2 x 10~3 M (250%) y AMPc 10-4 M (150%). La concomitante disminucion 3en el contenido de triglicéridos del hepatopâncreas en presencia de dopamina y AMPc sugiere la existencia de una relacion entre el incremento de la actividad de lipasa y la posible movilizacion de réservas sugiriendo el roi de estas mensajeros qui'micos como componente de las vfas de serialization involucrados en la regulation del metabolismo de lipidos.

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Section: Discussionsupporting

confidence: 91%

Lipase activity sensitive to dopamine, glucagon and cyclic AMP in the hepatopancreas of the euryhaline burrowing crab Neohelice granulata (Dana, 1851) (Decapoda, Grapsidae)

Michiels

Valle

Mañanes

2015

Crustac

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“…Biogenic amines, including octopamine, tyramine, dopamine, and serotonin are crucial neurotransmitters and/or neuromodulators, which modulate various physiological and behavioral responses in C. elegans. In particular, serotonin signaling is known to adjust food intake and maintain energy homeostasis based on nutrient availability 9,61,62 ; octopamine is shown to be involved in the neuronal regulation of longevity and mitochondrial dynamics 63 ; and dopamine has been reported to affect fat mobilization 64 . However, the role of tyramine in metabolic regulation remains unclear.…”

Section: Discussionmentioning

confidence: 99%

Olfactory specificity regulates lipid metabolism through neuroendocrine signaling in Caenorhabditis elegans

et al. 2020

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Olfactory and metabolic dysfunctions are intertwined phenomena associated with obesity and neurodegenerative diseases; yet how mechanistically olfaction regulates metabolic homeostasis remains unclear. Specificity of olfactory perception integrates diverse environmental odors and olfactory neurons expressing different receptors. Here, we report that specific but not all olfactory neurons actively regulate fat metabolism without affecting eating behaviors in Caenorhabditis elegans, and identified specific odors that reduce fat mobilization via inhibiting these neurons. Optogenetic activation or inhibition of the responsible olfactory neural circuit promotes the loss or gain of fat storage, respectively. Furthermore, we discovered that FLP-1 neuropeptide released from this olfactory neural circuit signals through peripheral NPR-4/neuropeptide receptor, SGK-1/serum-and glucocorticoid-inducible kinase, and specific isoforms of DAF-16/FOXO transcription factor to regulate fat storage. Our work reveals molecular mechanisms underlying olfactory regulation of fat metabolism, and suggests the association between olfactory perception specificity of each individual and his/her susceptibility to the development of obesity.

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“…All strains were obtained from the Caenofrhabditis Genetics Center. For all experiments, synchronized L1 populations were acquired through hypochlorite treatment of gravid adults (Barros et al, 2014 ).…”

Section: Methodsmentioning

confidence: 99%

Lifespan Extension Induced by Caffeine in Caenorhabditis elegans is Partially Dependent on Adenosine Signaling

Bridi

Barros

Sampaio

et al. 2015

Front. Aging Neurosci.

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Caffeine is a widely used psychoactive substance. Studies have shown that caffeine may play a protective role in aging-associated disorders. However, the mechanisms by which caffeine modulates aging are not yet clear. In this study, we have shown that caffeine increases Caenorhabditis elegans lifespan, delays its larval development, reduces reproduction and body length. These phenotypes were partly reversed by worm’s exposure to adenosine, which suggest a putative common target. Moreover, they were dependent on a functional insulin/IGF-1-like pathway. Our results may shed light on new genetic determinants of aging.

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Dopamine Signaling Regulates Fat Content through β-Oxidation in Caenorhabditis elegans

Cited by 22 publications

References 55 publications

Lipase activity sensitive to dopamine, glucagon and cyclic AMP in the hepatopancreas of the euryhaline burrowing crab Neohelice granulata (Dana, 1851) (Decapoda, Grapsidae)

Lipase activity sensitive to dopamine, glucagon and cyclic AMP in the hepatopancreas of the euryhaline burrowing crab Neohelice granulata (Dana, 1851) (Decapoda, Grapsidae)

Olfactory specificity regulates lipid metabolism through neuroendocrine signaling in Caenorhabditis elegans

Lifespan Extension Induced by Caffeine in Caenorhabditis elegans is Partially Dependent on Adenosine Signaling

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