Dopamine Dynamics and Signaling in &lt;i&gt;Drosophila&lt;/i&gt;: An Overview of Genes, Drugs and Behavioral Paradigms

Yamamoto, Shinya; Seto, Elaine S.

doi:10.1538/expanim.63.107

Cited by 136 publications

(165 citation statements)

References 133 publications

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“…We also screened for alterations in the color of bristles and cuticle to permit identification of genes involved in dopamine synthesis, secretion, metabolism, or melanization (Yamamoto and Seto, 2014) (Figure S2D). In addition, we selected mutations that affect wing morphogenesis to isolate genes that regulate core signaling pathways, including Notch, Wnt, Hedgehog, and BMP/TGF-β (Bier, 2005) (Figure S2E–J).…”

Section: Resultsmentioning

confidence: 99%

A Drosophila Genetic Resource of Mutants to Study Mechanisms Underlying Human Genetic Diseases

Yamamoto

Jaiswal

Charng

et al. 2014

Cell

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341

367

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Summary Invertebrate model systems are powerful tools for studying human disease owing to their genetic tractability and ease of screening. We conducted a mosaic genetic screen of lethal mutations on the Drosophila X-chromosome to identify genes required for the development, function, and maintenance of the nervous system. We identified 165 genes, most of whose function has not been studied in vivo. In parallel, we investigated rare variant alleles in 1,929 human exomes from families with unsolved Mendelian disease. Genes that are essential in flies and have multiple human homologs were found to be likely to be associated with human diseases. Merging the human datasets with the fly genes allowed us to identify disease-associated mutations in six families and to provide insights into microcephaly associated with brain dysgenesis. This bidirectional synergism between fly genetics and human genomics facilitates the functional annotation of evolutionarily conserved genes involved in human health.

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Section: Resultsmentioning

confidence: 99%

A Drosophila Genetic Resource of Mutants to Study Mechanisms Underlying Human Genetic Diseases

Yamamoto

Jaiswal

Charng

et al. 2014

Cell

Self Cite

341

367

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“…Dopaminergic pathways are known to reside at the core of reward processing in mammals and insects (Perry & Barron ), and in our study dopamine N‐acetyltransferase and dopamine/ecdysteroid receptor were in modules that were responsive to food, food type or food value. These two genes do not have mammalian orthologs, but they are key components of DA signaling in insects (Yamamoto & Seto ). DA signaling in the nucleus accumbens is also known to partially regulate food valuation in mammals (de Araujo ).…”

Section: Discussionmentioning

confidence: 99%

Brain regions and molecular pathways responding to food reward type and value in honey bees

McNeill

Kapheim

Brockmann

et al. 2016

Genes Brain and Behavior

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The ability of honey bees to evaluate differences in food type and value is crucial for colony success, but these assessments are made by individuals who bring food to the hive, eating little, if any, of it themselves. We tested the hypothesis that responses to food type (pollen or nectar) and value involve different subsets of brain regions, and genes responsive to food. mRNA in situ hybridization of c-jun revealed that brain regions responsive to differences in food type were mostly different from regions responsive to differences in food value, except those dorsal and lateral to the mushroom body calyces, which responded to all three. Transcriptomic profiles of the mushroom bodies generated by RNA sequencing gave the following results: (1) responses to differences in food type or value included a subset of molecular pathways involved in the response to food reward; (2) genes responsive to food reward, food type and food value were enriched for (the Gene Ontology categories) mitochondrial and endoplasmic reticulum activity; (3) genes responsive to only food and food type were enriched for regulation of transcription and translation; and (4) genes responsive to only food and food value were enriched for regulation of neuronal signaling. These results reveal how activities necessary for colony survival are channeled through the reward system of individual honey bees.

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“…Further, we assessed MDPV-induced behaviors in Drosophila melanogaster , specifically focusing on known DAT-associated behaviors. Drosophila is a powerful genetic model for studying behaviors that are associated with DA as well as promoted by psychostimulants 31–33 , as several genes that regulate DA transport, synthesis, and signaling are conserved between flies and humans 34 .…”

Section: Introductionmentioning

confidence: 99%

Atypical dopamine efflux caused by 3,4-methylenedioxypyrovalerone (MDPV) via the human dopamine transporter

Shekar

Aguilar

Galli

et al. 2017

Journal of Chemical Neuroanatomy

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Synthetic cathinones are similar in chemical structure to amphetamines, and their behavioral effects are associated with enhanced dopaminergic signaling. The past ten years of research on the common constituent of bath salts, MDPV (the synthetic cathinone 3,4-methylenedioxypyrovalerone), has aided the understanding of how synthetic cathinones act at the dopamine (DA) transporter (DAT). Several groups have described the ability of MDPV to block the DAT with high-affinity. In this study, we demonstrate for the first time, a new mode of action of MDPV, namely its ability to promote DAT-mediated DA efflux. Using single cell amperometric assays, we determined that low concentrations of MDPV (1 nM) can cause reverse transport of DA via DAT. Notably, administration of MDPV leads to hyperlocomotion in Drosophila melanogaster. These data describe further how MDPV acts at the DAT possibly paving the way for novel treatment strategies for individuals who abuse bath salts.

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Dopamine Dynamics and Signaling in <i>Drosophila</i>: An Overview of Genes, Drugs and Behavioral Paradigms

Cited by 136 publications

References 133 publications

A Drosophila Genetic Resource of Mutants to Study Mechanisms Underlying Human Genetic Diseases

A Drosophila Genetic Resource of Mutants to Study Mechanisms Underlying Human Genetic Diseases

Brain regions and molecular pathways responding to food reward type and value in honey bees

Atypical dopamine efflux caused by 3,4-methylenedioxypyrovalerone (MDPV) via the human dopamine transporter

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