Donor Tissue Preparation for Descemet Membrane Endothelial Keratoplasty: An Updated Review

Abstract

An overview of the current harvesting techniques available for DMEK may benefit corneal surgeons and eye banks in choosing the best approach for each specific user.

The feasibility and efficacy of preparing porcine Descemet’s membrane endothelial keratoplasty (DMEK) grafts by two techniques: An ex‐vivo investigation for future xeno‐DMEK

“…Moreover, the endothelial cells were preserved better after the liquid bubble technique. However, other studies reported the contrary result that manual stripping technique was similar or better than liquid bubble technique . Nevertheless, the success rates were similar between the 2 techniques, so it is hard to judge which technique is more reliable and suitable for porcine corneas.…”

“…However, other studies reported the contrary result that manual stripping technique was similar or better than liquid bubble technique. 32,33 Nevertheless, the success rates were similar between the 2 techniques, so it is hard to judge which technique is more reliable and suitable for porcine 35 Considering that, we investigated the correlation in the porcine DM, and the result also indicated the same tendency. However, the porcine DMEK rolls came from WZS-pigs with different ages were all triangle-shaped, and the tightness of the rolls did not vary with their thickness.…”

A comparative study on different Descemet membrane endothelial keratoplasty graft preparation techniques

“…There are several graft excision techniques that have been published in literature with advantages and disadvantages as recently reviewed and reported by Birbal et al. (). The limitation of this study was that we were only able to get data on five techniques that are routinely prepared by our eye bank and collaborators.…”

“…Using preprepared tissue may, therefore, facilitate smoother surgery. As there are several possible techniques for DMEK graft preparation (Birbal et al 2018), we studied few methods that are routinely used and compared them to find out the most suitable technique for DMEK graft preparation. Hence, we selected three different ways of stripping and two bubble techniques and compared multiple parameters.…”

“…However, these kind of tissues can only be stripped and cannot be bubbled as the bubble will pop out and the tissue will be torn or the liquid will leak from the incision site never allowing a bubble generation. There are several graft excision techniques that have been published in literature with advantages and disadvantages as recently reviewed and reported by Birbal et al (2018). The limitation of this study was that we were only able to get data on five techniques that are routinely prepared by our eye bank and collaborators.…”