Donor pretreatment with FK506 reduces reperfusion injury and accelerates intestinal graft recovery in rats

Oltean, Mihai; Pullerits, Rille; Zhu, Changlian; Blomgren, Klas; Hallberg, Eva; Olausson, Michael

doi:10.1016/j.surg.2006.11.005

Cited by 35 publications

(54 citation statements)

References 43 publications

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“…Of particular interest is the fact that donor pretreatment with tacrolimus can reduce graft proinflammatory activation and neutrophil inflammation, exhibiting milder reperfusion injury as well as accelerated morphologic and functional recovery [100]. Besides supposed mechanisms, such as inhibition of early NF-κB activation and augmentation of heat shock protein (HSP)-72 synthesis [100], tacrolimus has been shown to induce vasodilation by decreasing intracellular Ca(2+) levels and thus to reduce ischemia-reperfusion injury by attenuating the risk of vasospasm and thrombosis in small bowel transplantation [101].…”

Section: Pre-and Post-conditioning Maneuversmentioning

confidence: 99%

“…Besides supposed mechanisms, such as inhibition of early NF-κB activation and augmentation of heat shock protein (HSP)-72 synthesis [100], tacrolimus has been shown to induce vasodilation by decreasing intracellular Ca(2+) levels and thus to reduce ischemia-reperfusion injury by attenuating the risk of vasospasm and thrombosis in small bowel transplantation [101].…”

Section: Pre-and Post-conditioning Maneuversmentioning

confidence: 99%

See 1 more Smart Citation

Intestinal ischemia/reperfusion: microcirculatory pathology and functional consequences

Vollmar

Menger

2010

Langenbecks Arch Surg

228

153

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Section: Pre-and Post-conditioning Maneuversmentioning

confidence: 99%

Section: Pre-and Post-conditioning Maneuversmentioning

confidence: 99%

Intestinal ischemia/reperfusion: microcirculatory pathology and functional consequences

Vollmar

Menger

2010

Langenbecks Arch Surg

228

153

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“…FK506 have been proved to inhibit transplant rejection and prolong the survival of allografts by inhibiting the infiltration of neutrophils (27,28). So the inhibition role of FK506 further confirmed the role of neutrophils in promoting rejection of OMT.…”

Section: Discussionmentioning

confidence: 99%

Neutrophils infiltration is early event of an oral mucosal xenotransplantation model

Wang

Tao²,

Xiang³

et al. 2011

Med Oral

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Introduction: As important effector cells of the innate immune system, neutrophils are involved in rejection of solid organ and/or tissue transplants. But their role in rejection of oral mucosa transplantation (OMT) remains unclear. The aim of this study is to observe the spatial-temporal change of neutrophils during acute rejection of OMT. Methods: In a rat model of oral mucosal xenotransplantation, myeloperoxidase (MPO), an indicator of influx of neutrophils was detected by technique of ELISA on day 7 and 30 (D7, D30) of posttransplantation. Results: On D7, MPO level (6.183±0.416, ×10 2 ng/mg) in the OMT group was significantly higher than in trauma (0.681±0.073, ×10 2 ng/mg) and normal controls (0.262±0.043, ×10 2 ng/mg) (P<0.001, respectively), and this level was found to correlate with the index of submandibular lymph nodes (ILN), an indicator of inflammation of rejection (r=0.909, P<0.05). Moreover, this level was decreased significantly under FK506 treatment (2.103±0.146, ×10 2 ng/mg, P= 0.005). On D30, MPO in the OMT group (1.063±0.096, ×10 2 ng/mg) was lower significantly than that on D7 (P<0.001), although this level was still higher that of normal controls on D30 (0.532±0.112, ×10 2 ng/ mg, P = 0.042). Conclusion: Neutrophils infiltration was an early event of OMT, which may play important roles on acute rejection of OMT.

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“…In general terms, it is used to study intestinal ischemia-reperfusion injury, graft rejection and assess immunosuppressive therapies [2][3][4]. SBTx in rats is a feasible, replicable and inexpensive procedure when compared to large animals models [5].…”

Section: Introductionmentioning

confidence: 99%

Small bowel transplantation in rats, A multicenter experience summarizing the pitfalls to be overcome

Stringa¹,

Moreno²,

Lausada³

et al. 2017

Trends in Transplant

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Small Bowel transplantation in rats is a highly complex microsurgical procedure because several technical complications may lead to recipient mortality and transplant failure. Our aim was to report the most common complications associated with orthotopic and heterotopic intestinal transplantation in rats in order to identify the "pitfalls" of the procedure and prevent them. A retrospective multicenter study was performed. All participant centers have established rodent transplant procedures and trained surgeons. Two hundred ninety-three complications from 264 unsuccessful intestinal transplants were reported, representing an overall failure rate of 15% of the procedures performed. Recipient complications were most frequent than donor (257 vs. 36 p<0.0001). Excessive surgical time (11/36); severe hemorrhage (12/36) and inappropriate infusion of the preservation solution in the intestinal graft (11/36) were the most common donor complications. Arterial anastomosis bleeding (50/257), venous anastomosis bleeding (35/257) and portal vein stenosis (26/257) were the most common intraoperative complications in the recipient. To maximize success rate, surgeons should optimize time and avoid bleeding during graft dissection in the donor surgery. After performing a bloodless vascular anastomosis an adequate post-operative management of the animal is mandatory to guarantee survival.

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Donor pretreatment with FK506 reduces reperfusion injury and accelerates intestinal graft recovery in rats

Cited by 35 publications

References 43 publications

Intestinal ischemia/reperfusion: microcirculatory pathology and functional consequences

Intestinal ischemia/reperfusion: microcirculatory pathology and functional consequences

Neutrophils infiltration is early event of an oral mucosal xenotransplantation model

Small bowel transplantation in rats, A multicenter experience summarizing the pitfalls to be overcome

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