Donor neutrophil function after plateletpheresis

Western, Karoline H.; Videm, Vibeke

doi:10.1046/j.1537-2995.2000.40111414.x

Cited by 6 publications

(6 citation statements)

References 27 publications

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“…insignificant increase in absolute monocyte, lymphocyte subsets count, and WBC count. This was partially in agreement with Western and Videm reported an insignificant change in the donors mean leukocyte counts [31]. In contrast; Prior and coworkers found a transient drop in the absolute lymphocyte counts and T cells in healthy cytapheresis donors (mostly regular donors) during cardiopulmonary bypass [32].…”

Section: Discussionsupporting

confidence: 86%

“…In this study, we found that the expression of (CD11c & CD42a), (CD61), (CD11c), (IgG1) and (IgG2) postdonation was significantly increased on monocytes but with insignificant changes in the expression of (CD11c & CD42a), (CD61), and (CD11c) on neutrophil. However, there were significant increase in post donation expression of (IgG1 & IgG2a) and (IgG2a & CD41) on both monocytes and neutrophil, this was in agreement with Western and Videm [31] who found slight neutrophil function change after apheresis owing to contact between neutrophils and the apheresis foreign surface found to be a bio-tolerant procedure and also in agreement with Ahn and coworkers who established that activated platelets bind preferably to monocytes to form the plateletmonocyte complex (PMCs) [34]. This can be explained by the fact that during the apheresis procedure, the donor blood was exposed to an artificial membrane and external forces such as centrifugation that might activate platelets and leukocytes [35].…”

Section: Discussionsupporting

confidence: 85%

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Hematological cellular alterations in plateletpheresis donors

Fayed¹,

Ali

Eldin

2018

Int J Blood Transfus Immunohematol

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Aims: Blood contact with foreign surfaces in the apheresis systems may activate many cell types that return back to the donor. However, the safety issue regarding post-procedure platelet activation is not well assessed. We aimed to evaluate the alterations in hematological parameters and to explore the formation of platelets leukocytes aggregates (PLAs) and/or complexes in healthy donors who underwent first-time plateletpheresis procedure. Methods: Blood from 100 healthy donors were assessed by BD FACS Calibur flow cytometer for: a) detection of PLAs using (CD41, CD42b, CD61) antibodies against platelets surface molecules to detect its expression on neutrophils, monocytes, lymphocytes, and b) evaluation of red cell mechanical fragility (RBC-MF). Results: After donation a significant decrement of donor blood cell counts; the percent (%) reduction in hemoglobin (Hb) 7.9(5.1-9.2) (p = 0.017), hematocrit (Hct) 6(2.72-7.81) (p = 0.043) %, residual red cells (p = 0.016), platelet count (PLT) 22.7(9.5-32) (p = 0.031) together with a significant increase in the MPV (p = 0.001), the absolute neutrophil (p = 0.026) and lymphocyte

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Section: Discussionsupporting

confidence: 86%

Section: Discussionsupporting

confidence: 85%

Hematological cellular alterations in plateletpheresis donors

Fayed¹,

Ali

Eldin

2018

Int J Blood Transfus Immunohematol

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“…Although, there was a significant increase in postdonation expression of (IgG1 and IgG2a) and (IgG2a and CD41) on both monocytes and neutrophil. 21,22 Rinder et al showed that the modification of lymphocyte count was reached to normal level within 3 days after the extracorporeal process. 23 However, we had not assessed neutrophil, lymphocyte, and monocyte but observed WBC increasing by Trima Accel and MCS+, and CD4, CD25, and TGF-beta gene were not significantly different in the two devices.…”

Section: Discussionmentioning

confidence: 99%

The effect of platelet apheresis collection on some immunological factors in donors using two different apheresis devices

et al. 2021

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Introduction:The aim of this study was to evaluate and compare two different apheresis and changes in some immunological factors in donors. Material and Methods:The cross-sectional study was performed from January 2017 to September 2018. Fifty six male blood donors were randomly divided into two groups. CD4, CD8, and CD25 markers by flow cytometry, and TGFBeta by real-time polymerase chain reaction (RT-PCR) method were done before and 7 days after the apheresis procedure. Independent Sample t-test, Mann-Whitney U Test, Wilcoxon signed ranked test, and Fisher exact test were used. Results: WBC in MCS+ group after donation is significantly higher than before donation (P < 0.05) but no significant difference was seen between MCS+ and Trima groups in these two indicators. But in CD4, CD25, and TGFBeta, there was no significant difference between the two groups. Conclusion: There was no significant difference on CD4, CD25, and TGFBeta gene 7 days after donation.

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“…in the investigation of transfusion-related acute lung injury (TRALI), in population studies or in the analysis of potential changes during pregnancy [122][123][124][125][126][127]. Moreover, functional and morphologic changes of circulating neutrophil cells during plateletpheresis were investigated using flow cytometry [128]. Such investigations were also performed to identify the optimal storage conditions for granulocytes [129].…”

Section: Granulocyte/monocyte Integrity and Immunologymentioning

confidence: 99%

Flow Cytometry in Transfusion Medicine: Development, Strategies and Applications

Greve¹,

Valet²,

Humpe³

et al. 2004

Transfus Med Hemother

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Nowadays, flow cytometry represents an essential tool for the daily work in laboratories for transfusion medicine. After cytophotometry of immobilized cells, flow cytometry was developed since the 1960s using moving fluorescence-stained cells. This technique enabled the determination of several thousand cells per second, e.g. from blood, bone marrow, or organ-associated cell suspensions. The introduction of monoclonal fluorescence-labeled antibodies in diagnostics has further accelerated the development of flow cytometry. In the meantime, numerous cellular and nuclear properties can simultaneously be measured on single cell level in automated devices at high speed and precision. Internal standards and quality control systems further increased the accuracy of flow cytometric analyses. Flow cytometry is therefore likewise suitable for routine diagnostics in patients, quality control of blood components and scientific purposes in transfusion medicine. Nevertheless, many flow cytometric applications are related to counting of rare events or detection of cells with low antigen expression. Therefore, several pre-analytic and analytic factors (e.g. erythrocyte lysing procedures, cell gating strategies, etc.) may essentially affect the accurate determination of cell numbers and functions. In this issue, TRANSFUSION MEDICINE AND HEMOTHERAPY starts a recurrent sequence of reviews focused on ‘Flow Cytometry in Transfusion Medicine’. In order to introduce the flow cytometry series, this review summarizes the development, principles, and strategies of flow cytometry as an increasing diagnostic tool in medical laboratories. Also, current and possibly future flow cytometric applications in transfusion medicine are epitomized, e.g. quality control of blood products, immunohematologic diagnostics, hematopoietic progenitor cell transplantation, adoptive immunotherapy, and further therapeutic applications. In following issues of this journal, reviews of notable authors will then focus on special applications and give more detailed insights into single diagnostic fields.

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Donor neutrophil function after plateletpheresis

Abstract: Neutrophil function was altered after apheresis, although to a very small extent, and contact between neutrophils and the foreign surface in the apheresis systems is found to be a biotolerant procedure.

Cited by 6 publications

References 27 publications

Hematological cellular alterations in plateletpheresis donors

Hematological cellular alterations in plateletpheresis donors

The effect of platelet apheresis collection on some immunological factors in donors using two different apheresis devices

Flow Cytometry in Transfusion Medicine: Development, Strategies and Applications

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