Donor Brain Death Leads to a Worse Ischemia-Reperfusion Injury and Biliary Injury After Liver Transplantation in Rats

Chen, Sanyang; Fang, Hongbo; Li, Jie; Shi, Jian; Zhang, Jiakai; Wang, Zhihui; Cao, Shengli; Han, Yang; Pan, Jie; Tang, Hongwei; Zhang, Huapeng; Guo, Wenzhi; Zhang, Shuijun

doi:10.1016/j.transproceed.2019.10.012

Cited by 6 publications

(4 citation statements)

References 28 publications

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“…Male Sprague–Dawley rats weighing 250–280 g were purchased from Hunan SJA Laboratory Animal Company (Wuhan, China) and housed in specific-pathogen-free cages at a constant temperature and humidity, on a 12-hour light-dark cycle, with food and water intake ad libitum . Brain death (BD group) was induced as described previously (13). For the sham group, all procedures were the same except that the catheter was inserted into the epidural space but not ballooned.…”

Section: Methodsmentioning

confidence: 99%

Intestinal microbiota dysbiosis and liver metabolomic changes during brain death

Wang

Tao

Guo

et al. 2022

Preprint

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Background The causative link between brain death and intestinal microbiota dysbiosis is unclear, and the distortion in liver metabolism caused by brain death requires further exploration. Material and Methods A rat model of brain death was constructed and sustained for 9 hours. Intestinal contents and portal vein plasma were collected for microbiota sequencing and microbial metabolite detection. Liver tissue was resected to investigate metabolic alterations, and the results were compared with those of a sham group. Results Microbiota dysbiosis occurred at the family and genus levels after 9 hours of brain death. Microbial metabolites remained unchanged in both the intestinal contents and portal vein plasma. Liver metabolic function deteriorated, and the levels of 80% of the differential metabolites decreased in the brain-dead rats. Most of the differential metabolites were related to energy metabolism. Conclusions Brain death resulted in microbiota dysbiosis in rats; however, this dysbiosis did not alter microbial metabolites. Deterioration in liver metabolic function during extended periods of brain death may reflect a continuous worsening in energy deficiency.

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Section: Methodsmentioning

confidence: 99%

Intestinal microbiota dysbiosis and liver metabolomic changes during brain death

Wang

Tao

Guo

et al. 2022

Preprint

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“…The increasing number of studies of donor management research across the studied years shows that this is a rapidly evolving research area. The prevention of organ injury by in uencing the balance of pro-and anti-in ammatory mediators, targeting ischaemia-reperfusion and studying the role of the immune response to target recovery and repair has been studies in many pre-clinical settings [35][36][37][38][39][40] . However, there is limited translation of research of prevention of organ injury (and promotion of repair or recovery) into clinical practice during the period of donor management.…”

Section: Key Ndingsmentioning

confidence: 99%

Outcome Measures in Solid Organ Donor Management Research - a Systematic Review

Bera

Shah

English

et al. 2021

Preprint

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BackgroundTo systematically review published outcome measures across randomised controlled trials (RCTs) of donor management interventions. MethodsThe systematic review was conducted in accordance with recommendations by the Cochrane Handbook and PRISMA statement. We searched MEDLINE, EMBASE, CENTRAL, Web of Science as well as trial databases from 1980 to December 2019 for RCTs of donor management interventions. ResultsTwenty-two RCTs (n = 3432 donors) were included in our analysis. Fourteen RCTs (63.6%) reported a primary outcome relating to a single organ only. Eight RCTs primarily focused on aspects of donor optimisation in critical care. Thyroid hormones and methylprednisolone were the most commonly evaluated interventions (five and four studies, respectively). Only two studies, focusing on single organs (e.g. kidney), evaluated outcomes relating to other organs. The majority of studies evaluated physiological or biomarker-related outcomes. No study evaluated recipient health-related quality of life. Only one study sought consent from potential organ recipients. ConclusionsThe majority of RCTs evaluating donor management interventions only assessed single organ outcomes or effects on donor stability in critical care. There is a need for an evaluation of patient-centred recipient outcomes, and standardisation and reporting of outcome measures for future donor management RCTs. PROSPERO RegistrationCRD42018109487

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“…The increasing number of studies of donor management research across the studied years shows that this is a rapidly evolving research area. The prevention of organ injury by in uencing the balance of pro-and anti-in ammatory mediators, targeting ischaemia-reperfusion and studying the role of the immune response to target recovery and repair has been studies in many pre-clinical settings (34)(35)(36)(37)(38)(39). However, there is limited translation of research of prevention of organ injury (and promotion of repair or recovery) into clinical practice during the period of donor management.…”

Section: Key Ndingsmentioning

confidence: 99%

Outcome measures in solid organ donor management research: a systematic review

Bera

Shah

English

et al. 2021

British Journal of Anaesthesia

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BackgroundTo systematically review published outcome measures across randomised controlled trials (RCTs) of donor management interventions. MethodsThe systematic review was conducted in accordance with recommendations by the Cochrane Handbook and PRISMA statement. We searched MEDLINE, EMBASE, CENTRAL, Web of Science as well as trial databases from 1980 to December 2019 for RCTs of donor management interventions. ResultsTwenty-two RCTs (n = 3432 donors) were included in our analysis. Fourteen RCTs (63.6%) reported a primary outcome relating to a single organ only. Eight RCTs primarily focused on aspects of donor optimisation in critical care. Thyroid hormones and methylprednisolone were the most commonly evaluated interventions ( ve and four studies, respectively). Only two studies, focusing on single organs (e.g. kidney), evaluated outcomes relating to other organs. The majority of studies evaluated physiological or biomarker-related outcomes. No study evaluated recipient health-related quality of life.Only one study sought consent from potential organ recipients. ConclusionsThe majority of RCTs evaluating donor management interventions only assessed single organ outcomes or effects on donor stability in critical care. There is a need for an evaluation of patient-centred recipient outcomes, and standardisation and reporting of outcome measures for future donor management RCTs.

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Donor Brain Death Leads to a Worse Ischemia-Reperfusion Injury and Biliary Injury After Liver Transplantation in Rats

Cited by 6 publications

References 28 publications

Intestinal microbiota dysbiosis and liver metabolomic changes during brain death

Intestinal microbiota dysbiosis and liver metabolomic changes during brain death

Outcome Measures in Solid Organ Donor Management Research - a Systematic Review

Outcome measures in solid organ donor management research: a systematic review

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