Donor age negatively impacts adipose tissue-derived mesenchymal stem cell expansion and differentiation

Choudhery, Mahmood S; Badowski, Michael; Muise, Angela; Pierce, D. John; Harris, David T.

doi:10.1186/1479-5876-12-8

Cited by 427 publications

(416 citation statements)

References 38 publications

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“…In fact, there have been mixed results in the differentiation characteristics of these cells. It is likely that the age of the donor from which the stem cells are derived will affect the differentiation properties [74]. Together with our findings, it appears that the TM stem/ progenitor cells are present in both primate and non-primate animals.…”

supporting

confidence: 69%

Bovine Posterior Limbus: An Evaluation of an Alternative Source for Corneal Endothelial and Trabecular Meshwork Stem/Progenitor Cells

Grierson

Sheridan

et al. 2015

Stem Cells and Development

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A growing body of evidence has revealed that stem-like cells in the posterior limbus of the eye between the corneal endothelium (CE) and trabecular meshwork (TM) may be able to rejuvenate these tissues in disease. However, these cells have not been clearly defined and we have named them PET cells (progenitor cells of the endothelium and trabeculum). A good and inexpensive animal model for PET cells is lacking, so we investigated bovine eyes as an effective large tissue source. We showed the presence of stem/progenitor cells in the bovine CE, transition zone, and TM in situ. Floating spheres cultured from the CE and TM showed similar stem cell marker expression patterns. Both the CE and TM spheres were bipotent and highly proliferative, but with limited secondary sphere-forming capability. They were highly prone to differentiate back into the cell type of their tissue of origin. It is speculated that the PET cells become more tissue-specific as they migrate away from their niche. Here, we showed that PET cells are present in the posterior limbus of bovine eyes and that they can be successfully cultured and expanded. PET cells represent an attractive target for developing new treatments to regenerate both the CE and TM, thereby reducing the requirement for donor tissue for corneal transplant and invasive treatments for glaucomatous patients.

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supporting

confidence: 69%

Bovine Posterior Limbus: An Evaluation of an Alternative Source for Corneal Endothelial and Trabecular Meshwork Stem/Progenitor Cells

Grierson

Sheridan

et al. 2015

Stem Cells and Development

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“…This is difficult because it is not easy to get ovarian tissue, especially in younger women. We can only speculate on an ideal age based on the literature; this age could be under 30 years-old [34]. Moreover, our data showed that the female age did not affect the characteristics of PO-MSCs and that even in the oldest patient, aged 75 years, PO-MSCs expressed a high potential to differentiate into adipogenic in osteogenic cells.…”

Section: Discussionmentioning

confidence: 88%

“…It could be expected that aging negatively affects the characteristics of MSCs and has an impact on gene expression, although we did not observe this. Some studies have shown that aging increased senescence and reduced cell viability, proliferation and differentiation potential [34][35][36]. Furthermore, when performing in vitro studies, the long-term expansion of MSCs had a significantly negative impact on the characteristics of MSCs, regardless of donor age [35,37].…”

Section: Discussionmentioning

confidence: 99%

Putative mesenchymal stem cells isolated from adult human ovaries

Štimpfel

Cerkovnik

Novaković

et al. 2014

J Assist Reprod Genet

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Purpose The purpose of this study was to show that healthy adult human ovaries can be a source of cells showing typical MSCs characteristics under in vitro conditions. Methods and results The cells, which were isolated from ovarian cortex tissue and named putative ovarian mesenchymal stem cells (PO-MSCs), were compared to bone marrowderived MSCs (BM-MSCs) and to adult human dermal fibroblasts (HDFs). The results of a gene expression analysis using the Human Mesenchymal Stem Cell RT² Profiler™ PCR Array revealed that PO-MSCs were different than fibroblasts. They expressed most of the analyzed genes as BM-MSCs, although some genes were differentially expressed. However, the heterogeneity of PO-MSCs samples was revealed. The PO-MSCs expressed the characteristic genes related to MSCs, such as CD105, CD44, CD90, M-CAM, CD73 and VCAM1. In addition, the expression of markers CD44, CD90, M-CAM and STRO-1 was confirmed in PO-MSCs using immunocytochemistry. The PO-MSCs showed multipotent character, since they were able to differentiate into the cells of adipogenic, osteogenic, neural and pancreatic lineage. Conclusions Healthy adult human ovaries can harbour an interesting population of cells showing typical MSCs characteristics under in vitro conditions and for this reason we named these cells putative MSCs. These cells express genes encoding main MSCs markers and have an interesting differential potential. Based on these results, we propose PO-MSCs as a novel type of MSCs which share some similarities with BMMSCs. Nevertheless they show distinct and specific characteristics and are not fibroblasts.

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“…Recently, adipose tissue derived stem cells have been the most favourable cell source for research and clinical application because of their dominant characteristics (Bassi, 2012;Choudhery et al, 2014;Melief et al, 2013). Therefore, an important step forward for the treatment of diabetes would be to differentiate these cells into insulin-producing cells.…”

Section: Discussionmentioning

confidence: 99%

Production of islet-like insulin-producing cell clusters in vitro from adiposederived stem cells

Dang¹,

Bui²,

Pham³

et al. 2015

Biomed Res Ther

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Abstract-Diabetes mellitus is a high incidence disease that has increased rapidly in recent years. Many new therapies are being studied and developed in order to find an effective treatment. An ideal candidate is stem cell therapy. In this study, we investigated the differentiation of adipose derived stem cells (ADSCs) into pseudo-islets in defined medium in vitro, to produce large quantities of insulin-producing cells (IPCs) for transplantation. ADSCs isolated from adipose tissue were induced to differentiate into islet-like insulin-producing cell clusters in vitro by inducing medium DMEM/F12 containing nicotinamide, N2, B27, bFGF, and insulin-transferrin-selenite (ITS). Differentiated cells were analyzed for properties of IPCs, including storage of Zn 2+ by dithizone staining, insulin production by ELISA and immunochemistry, and beta cell-related gene expression by reverse transcriptase PCR. The results showed that after 2 weeks of differentiation, the ADSCs aggregated into cell clusters, and after 4 weeks they formed islets, 50-400 micrometers in diameter. These islet cells exhibited characteristics of pancreatic beta cells as they were positive for dithizone staining, expressed insulin in vitro and C-peptide in the cytoplasm, and expressed pancreatic beta cellspecific genes, including Pdx-1, NeuroD, and Ngn3. These results demonstrate that ADSCs can be used to produce a large number of functional islets for research as well as application.

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Donor age negatively impacts adipose tissue-derived mesenchymal stem cell expansion and differentiation

Cited by 427 publications

References 38 publications

Bovine Posterior Limbus: An Evaluation of an Alternative Source for Corneal Endothelial and Trabecular Meshwork Stem/Progenitor Cells

Bovine Posterior Limbus: An Evaluation of an Alternative Source for Corneal Endothelial and Trabecular Meshwork Stem/Progenitor Cells

Putative mesenchymal stem cells isolated from adult human ovaries

Production of islet-like insulin-producing cell clusters in vitro from adiposederived stem cells

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